Study on early diagnosis of epithelial ovarian cancer by analysis of plasma septin-9 and clusterin level

Abstract

The plasma levels of septin-9 and clusterin in ovarian cancer patients were abnormally elevated, which might be used as potential candidates of peripheral blood tumor biomarkers for early diagnosis of EOC and septin-9 might be related to distal metastases of EOC. The septin-9 might play the promotion role, which protein level relates to not only the distal metastases but also the prognosis of EOC. Due to the limit of sample volume, further enlargement of the sample size and set up of the follow-up system is in need to in-depth study the relationship between plasma protein concentration with the distal metastases, and further explore its correlation with the prognosis of EOC.

SEPT9 Expression in Hepatic Nodules: An Immunohistochemical Study of Hepatocellular Neoplasm and Metastasis

“…In fact, previous work involving various cancer patients, including those with CRC and HCC, has highlighted worse outcomes in patients with higher plasma methylated SEPT9 DNA levels and/or higher tissue SEPT9 expression. 17,19,22,24,34,35 Third, we found a positive correlation between SEPT9 staining and older age. Given the high degree of methylation that occurs during aging, this finding suggests DNA methylation most likely contributes to SEPT9 overexpression in HCC.…”

“…Detection of methylated SEPT9 DNA (mSEPT9) in plasma is a molecular-based blood test that was recently approved by the Food and Drug Administration (FDA) as an alternative non-invasive test for screening of colorectal carcinomas (CRC) and is currently commercially available. 14 Given the ubiquitous nature of SEPT9 and its involvement in numerous malignancies, many recent studies have investigated the utility of mSEPT9 DNA or SEPT9 protein assays for the early detection of head and neck, 15,16 ovarian, 17 breast, 18 cervical, 19 pancreatic, 20 and liver cancers [21][22][23][24][25] with promising results. For HCC, SEPT9 may have significant clinical implications as the current modalities for HCC diagnosis in cirrhotic patients using imaging and alpha fetoprotein (AFP) levels are limited by suboptimal sensitivities.…”

Structure and function of Septin 9 and its role in human malignant tumors

“…The mean level of plasma SEPT9 in epithelial ovarian carcinoma patients was significantly higher than that in healthy women. The mean level of plasma SEPT9 in epithelial ovarian cancer patients with a tumor family history or distant metastases was significantly higher than that of other patients[ 85 ].…”

The low doses of SWCNTs affect the expression of proliferation and apoptosis related genes in normal human astrocytes

“…1 ). These results clearly demonstrated that exposure of normal human astrocytes with low amounts of SWCNTs significantly up-regulated the expression of genes encoded the endoplasmic reticulum stress responsible proteins such as CLU, DNAJB9, and IGFBP3, which are increased in variable cancers ( Bache et al, 2015 , Canel et al, 2017 , Hou et al, 2019 , Johnson and Firth, 2014 , Liu et al, 2018 , Lyu et al, 2018 ). It is possible that SWCNTs introduce the endoplasmic reticulum stress signaling pathways like many other nanoparticles ( Barberet et al, 2017 , Cao et al, 2017 , Hu et al, 2018 , Simon et al, 2017 ).…”

“…These genes play an important role in the regulation of multiple metabolic processes and also participate in endoplasmic reticulum stress and cancerogenesis ( Amin-Wetzel et al, 2017 , Bache et al, 2015 , Lee et al, 2015 , Liu et al, 2018 ). Proteins, encoded by CLU and DNAJB9 genes represent cell stress chaperones, have pro-proliferative properties and are increased in variable cancers ( Bache et al, 2015 , Liu et al, 2018 , Lyu et al, 2018 ). Furthermore, protein DNAJB9 is the endoplasmic reticulum luminal co-chaperone ERdj4, which is a selective ERN1 (endoplasmic reticulum to nucleus signaling 1) repressor.…”