Study on Construction and Performance of Immobilized Enzyme Reactors by Carboxyl-functionalized Fe₃O₄

Abstract

The interface connection between carrier and enzyme can be improved by functionalizing the carrier of immobilized enzyme. This facilitates the formation of highly ordered two-dimensional arrangement of enzyme molecules on the surface of the carrier, thus improving the catalytic activity and operational stability of the enzymes. The surface of Fe3O4 modified with citric acid (CA-Fe3O4) is rich in carboxyl groups, which can be used as an excellent carrier due to its magnetic and easy separation characteristics. In this work, the adsorption method was more suitable for constructing enzyme reactors on the carrier of CA-Fe3O4, compared with covalent method.