“…Nagata et al reported the transfection of Saccharomyces cerevisiae protoplasts with liposome-encapsulated plasmid DNA, 8) and the delivery of 6-carboxyfluorescein into Sacch. cerevisiae protoplasts mediated by liposomes which were made of phospholipids extracted from the same yeasts was reported by Yamanaka et al 9 ) But the details of liposome protoplast interactions were left unknown. Therefore it seems valuable to find hether or not liposomes can introduce membrane-impermeable substances into yeast protoplasts, and if possible, to discover the factors which affect the efficiency of delivery of liposomal content into yeast protoplasts drastically.…”