Study on Biological Activity of Litsea monopetala From Panchthar District of Nepal

Khanal, Subash; Bhatt, Bishan Datt

doi:10.3126/jist.v25i2.33747

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Previous studies have also shown the good antioxidant activity and high phenolic content of L. monopetala . 46 Further evaluation of the molecular mechanism of anti-inflammatory activity is necessary to determine the mechanism of NO inhibition. However, the genus Litsea has been reported to possess a wide range of secondary metabolites, including alkaloids, sesquiterpenes, flavonoids, lactones, lignans, and essential oil.…”

Section: Discussionmentioning

confidence: 99%

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Lamichhane¹,

Sharma²,

Sapkota³

et al. 2023

JEP

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Introduction Herbal products have been widely used for the treatment of diseases throughout the ages. In this research, we investigated antioxidant, antibacterial, anti-adipogenic, and anti-inflammatory activities of methanolic extracts of five ethnomedicinally important plants; namely, Alnus nepalensis , Dryopteris sparsa , Artocarpus lacucha , Litsea monopetala , and Lyonia ovalifolia . Methods We investigated the DPPH free radical scavenging potential, sensitivity of selected bacterial strains towards the extracts using a disc diffusion assay, anti-inflammatory activity in RAW-264.7 cells, and anti-adipogenic activity by the ORO assay in 3T3-L1 preadipocytes. Results and discussion The extract of A. nepalensis showed significant antioxidant activity (IC 50 =4.838 µ g/mL), followed by A. lacucha , L. monopetala , and L. ovalifolia , exhibiting comparable IC 50 values to that of ascorbic acid (IC 50 =5.063 µ g/mL). Alnus nepalensis also showed good antibacterial activity in disc diffusion methods, with remarkable zones of inhibition in A. baumannii (14.66 mm) and P. mirabilis (15.50 mm) bacterial species. In addition, A. nepalensis was found to increase adipogenesis in 3T3-L1 cells, evidenced by increased lipid deposition in differentiated 3T3-L1 cells. A similar pattern of increased adipogenesis was observed on treatment with L. ovalifolia extracts. On the other hand, A. lacucha effectively reduced lipid deposition in 3T3-L1 cells at 100 µ g/mL (75.18±6.42%) by inhibiting adipogenesis, showing its potential use in the management of obesity. Furthermore, A. lacucha 100 µg/mL (15.91±0.277 µ M) and L. monopetala 75 µ g/mL (12.52±0.05 µ M) and 100 µg/mL (11.77±0.33 µ M) significantly inhibited LPS-induced nitric oxide production in RAW 264.7 cells. Also, A. nepalensis and L. ovalifolia inhibited NO production significantly, endorsing their anti-inflammatory potential. Conclusion The findings from these in-vitro studies suggest that the selected five plants possess remarkable antioxidant, antibacterial, anti-adipogenic, and anti-inflammatory activities. This study opens the door to conduct further advanced in-vivo experiments to find possible le...

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Section: Discussionmentioning

confidence: 99%

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Lamichhane¹,

Sharma²,

Sapkota³

et al. 2023

JEP

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“…Phytochemical screening of Ipomoea species also revealed the presence of tannins, saponins, flavonoids, triterpenes, phenols and alkaloids. Antimicrobial sensitivity results showed that methanolic extract had promising antibacterial and fungal activity at the highest concentration of 500 mg/ml [24].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Antimicrobial Activity of Cuscuta reflexa ROXB. and Few Members of Family Convolvulaceae

Noshad,

Ajaib,

Kiran

2023

CTO

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Antioxidant activity of 12 species, of which one was Cuscuta reflexa Roxb. which belongs to the family of Cuscutaceae as well as 11 of Convolvulaceae, collected from district Bhimber, Mirpur and Kotli were measured by using ABTS, DPPH and FRAP assay. The total phenol and flavonoid contents of the whole plant of all the selected species were investigated. Ipomoea eriocarpa showed a comparatively higher amount of total polyphenols (0.98 ± 0.073 mg/gdw). The maximum DPPH value was shown by Ipomoea carnea Jacq (5.6%) and the minimum value was shown by Convolvulus arvensis L. extracts (3.0 %). ABTS was the highest by Convolvulus prostrastus and the lowest by Ipomoea carnea. In FRAP assay, the highest value was 2.75 by Ipomoea hederacia and the lowest value was 0.31 by I. arachnosperma Welw. The antifungal and antibacterial activity of the methanolic extracts of the whole plant of all the selected species was carried out by using both bacterial and fungal strains. Two gram positive bacteria namely Staphylococcus aureus and Bacillus subtilis, two gram negative bacteria namely E. coli and Pseudomonas aeruginosa, two fungal strains of Aspergillus niger and Aspergillus oryzae were used to carry out antimicrobial activity. For the comparison of inhibition zones showed by plant extracts against bacterial and fungal strains. Erythromycin, tetracycline and cefoparazone were used as standard for bacterial strains. Moreover, fungivine and Terbinafine were used as standard antibiotics against fungal strains. Among fungal strains, the highest zone of inhibition was shown by Cuscuta reflexa Roxb. extract against Aspergillus niger, that is 5.55±0.3, while the lowest zone of inhibition, that is, 0.60±0.28mm was shown by Evolvulus alsinoides (L.) extract against Aspergillus oryzae. Plants from the Convolvulaceae family have had frequent traditional uses for different therapeutic purposes. Furthermore, all plants selected for this study showed strong antimicrobial, antifungal, and antioxidant activity. Their further detailed phytochemical studies can help to develop plant-based therapeutic agents

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“…Furthermore, an arabinoxylan has been documented in the mucilage of L. monopetala leaves, contributing to the comprehensive understanding of its phytochemical composition. 4,5 In our study, we focused our research on L. monopetala and we, here in, report the presence of two polyprenols, from an ethyl acetate extract of the leaf of the plant.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Polyprenols from Litsea monopetala (Roxb.) Pers.

Kamal,

Ashrafi,

Sultana

et al. 2024

Dhaka Univ. J. Pharm. Sci

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Litsea monopetala (Roxb.) Pers. (Family: Lauraceae) is a plant that holds substantial ethnobotanical significance due to its traditional and cultural importance. In this study, we conducted an extensive chemical investigation on L. monopetala, a plant native to the regions including Nepal, India, and Bangladesh. Two unique secondary metabolites were isolated and characterized from ethyl acetate (EtOAc) extract of the leaves of L. monopetala which were identified as tri-trans poly-cis prenol-15 (1), and its acetylated derivative tri-trans poly-cis prenol-15 (2). The structures of the isolated compounds were determined through in-depth analysis of their highresolution 1H NMR, 13C NMR, COSY, HSQC, and HMBC spectroscopic data, along with comparison to existing published data. Both the compounds are isolated from the plant for the first time. Further studies are recommended to analyze the potential bioactivity associated with the molecules. Dhaka Univ. J. Pharm. Sci. 23(1): 63-68, 2024 (June)

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Study on Biological Activity of Litsea monopetala From Panchthar District of Nepal

Cited by 4 publications

References 9 publications

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Antioxidant and Antimicrobial Activity of Cuscuta reflexa ROXB. and Few Members of Family Convolvulaceae

Isolation and Characterization of Polyprenols from Litsea monopetala (Roxb.) Pers.

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