Study on Baohe Pills Regulating Intestinal Microecology and Treating Diarrhea of High-Fat and High-Protein Diet Mice

Abstract

Objective. To investigate the effects of Baohe pills on intestinal microorganisms and enzyme activities in mice with a high-fat and high-protein diet. Methods. 45 KM male mice were randomly divided into the control group, the high-fat and high-protein diet group, and the Baohe pill intervention group. The mice in the high-fat and high-protein diet group and the Baohe pill intervention group were fed with the self-made high-fat and high-protein diet as the sole food source of the mice, and the mice in the control group were fed with the normal diet. Starting from the 7th day of the feed intervention, mice in the Baohe pill intervention group were given 0.28 g/mL of Baohe pill decoction twice a day at the dose of 6.67 g/(kg·day), each time of 0.35 mL for 6 days. Mice in the control group and the high-fat and high-protein diet group were given the same amount of distilled water by gavage. The general state of mice in each group was observed, and the changes of intestinal microorganisms and intestinal enzyme activities were analyzed by culturable microorganism technology and intestinal functional enzyme detection technology. Results. The excrement of mice fed with a high-fat and high-protein diet was relatively thin and wet, and the Baohe pill intervention could not improve the symptoms well. In the high-fat and high-protein diet group, the number of bacteria, Escherichia coli, Lactobacillus, and Bifidobacterium, was significantly lower than that in the control group ( P < 0.01 ). Baohe pills could obviously increase the high-fat, high-protein diet for the number of culturable microorganisms in mice, the total number of bacteria, and the number of Bifidobacteria in the most significant ( P < 0.01 ), but the number of bacteria, Escherichia coli, and the Lactobacillus are still significantly lower than the control group ( P < 0.01 ). In terms of enzyme activity, both contents and mucosa, the Baohe pill could improve the activities of amylase, protease, sucrase, and lactase in high-fat and high-protein diet mice, which were significantly different from the control group ( P < 0.05 ). In terms of microbial activity, the intestinal contents of high-fat and high-protein mice were lower than those of the control group, while the intestinal mucosa was higher than that of the control group, but the difference was not significant ( P > 0.05 ). Baohe pills could improve the intestinal contents and intestinal mucosal microbial activity of mice, and the difference was significant in the high-fat and high-protein diet group ( P < 0.05 ). Discussion. A high-fat and high-protein diet can destroy the physiological balance of the body, which is mainly reflected in the disturbance of intestinal flora and the decrease of some enzyme activities and microbial activity. Baohe pills can restore the number of intestinal flora to a certain extent and improve the activities of various digestive enzymes including protease and amylase.

Dose of sucrose affects the efficacy of Qiweibaizhu powder on antibiotic-associated diarrhea: Association with intestinal mucosal microbiota, short-chain fatty acids, IL-17, and MUC2

“…Preparation of FDA stock solution and FDA reaction solution: 2 mg of FDA is mixed with 1 mL of acetone to produce 2 mg/mL of FDA stock solution, reserved at 2-8°C and protected from light, added FDA stock solution to sterilized PBS (pH = 7.6) so that the final concentration of FDA reaction solution is 10 μg/mL ( Guo et al, 2022 ; Li X. et al, 2022 ).…”

“…Experimental group: Added 2 mL FDA reaction solution and 50 μL sample into a dry sterilized tube, and the mixture was incubated at 24°C for 90 min, subsequently removed, and added 2 mL acetone was to terminate the reaction. Finally, all samples were determined three times in parallel by UV spectrophotometer at 490 nm, and the microbial activity per unit mass of the sample is expressed as A 490 value ( Guo et al, 2022 ; Li X. et al, 2022 ).…”

Diarrhea accompanies intestinal inflammation and intestinal mucosal microbiota dysbiosis during fatigue combined with a high-fat diet

“…The dietary composition was found to influence the incidence and progression of diarrhea [ 6 , 7 ]. Protein and a high-fat diet were associated with diarrhea, significantly reduced Lactobacillus and Bifidobacterium , and decreased mouse digestive enzyme activity and microbial activity [ 8 , 9 ]. Lard is a common edible oil used by chinese residents and decreased intestinal microbial diversity in mice fed lard [ 10 ].…”

Baohe pill decoction treats diarrhea induced by high-fat and high-protein diet by regulating lactase-producing bacteria in intestinal mucosa

“…The main effective components include hesperidin, forsythin, oleanolic acid, and ursolic acid ( He et al, 2020 ). Previous studies have shown that BPD plays a good role in the repair of intestinal mucosal injury, the recovery of intestinal microorganisms and the regulation of intestinal enzymes (including lactase; You et al, 2021 ; Guo et al, 2022 ; Huang et al, 2022 ). For example, BPD can treat diarrhea caused by HFHPD by increasing the abundance of the lactase-producing bacterium Lactobacillus spp., while reducing the abundance of the opportunistic pathogen Ralstonia ( Huang et al, 2022 ).…”