Study on Assays for the Detection of Serum Antibodies to Measles from Children and Its Standardization

Numazaki, Kei

doi:10.15344/2455-2364/2015/108

Cited by 3 publications

(3 citation statements)

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“…Serum antibodies to measles, varicella and mumps were determined by EIA and those to rubella were determined by HI assay [2]. Until the year of 2009 antibodies to varicella and mumps had been determined by complement-fixation (CF) test and by HI assay.…”

Section: Prevalence Of Serum Antibodies Against Vpd In Japanese Students Of Co-medical Fieldsmentioning

confidence: 99%

“…Although the hemagglutination-inhibition (HI) test used to be the most commonly used laboratory test for assessing immunity, more sensitive enzyme immunoassay (EIA) assays are now used in most diagnostic laboratories [2]. All persons who work in health-care facilities should be immune to MMRV and have a responsibility to avoid transmitting infections and thereby causing harm to patients.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of serum antibodies against vaccine-preventable diseases in Japanese students and its advisory standard of vaccination

Numazaki¹

2021

Clin Res Trials

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Although measles, mumps, rubella, and varicella (MMRV) are vaccine-preventable diseases common in childhood, the incidence of these viral infections has recently increased in the adult Japanese population. For prevention against infections in medical facilities, it is important to assess the immunity against these viruses and to achieve immunocompetence by vaccination. The purpose of this study was to determine the need for MMRV vaccinations in Japanese students of co-medical fields by means of a seroprevalence survey for these viral infectious diseases and to recommend vaccination.Status of the students attending to International University of health and welfare, Otawara, Tochigi, Japan were surveyed in 2008 to 2011 academic year for vaccination and past medical history by self-administered questionnaire and the serum antibody prevalence for enrollment concerning measles, rubella, varicella and mumps. Majority of the students in all years had immunity for measles, rubella and varicella but not for mumps by EIA assy. Although positive rates of measles antibody had risen since 2009, which of rubella was extremely low in the year of 2011. Serum antibody titers of rubella were lower than those of measles in all years. Freshman of 2008 showed highest antibody titers against measles and rubella. A high prevalence rate for the varicella antibody was observed in subjects of 2010 and 2011. The prevalence rate for the mumps antibody was lower in subjects of 2010 and 2011.Vaccinations in Japan are not enforced and like the United States for final vaccination activities entrusted to guardians and personal perceptions and judgment, limits in encouraging vaccination for legal status. The results of this study suggest that the forth stage of measles and rubella mixed regular vaccination targeted since 2008 in Japan was effective. Also determined what should be done the encouraging vaccination based on specific past medical history, vaccination histories (including dates and times) about encouraging vaccination, appropriate for the individual.

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Section: Prevalence Of Serum Antibodies Against Vpd In Japanese Students Of Co-medical Fieldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence of serum antibodies against vaccine-preventable diseases in Japanese students and its advisory standard of vaccination

Numazaki¹

2021

Clin Res Trials

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“…HI was utilized for a number of applications in diagnostic virology. The assay was used for serosurveillance of influenza A (H1N1) pdm09 virus [ 158 ] and measles virus [ 159 ]. In one study, HI assay was applied to assess the efficacy of pandemic influenza vaccine [ 160 ].…”

Section: Immunological Diagnostic Techniques Of Medical Virusesmentioning

confidence: 99%

Molecular and Immunological Diagnostic Techniques of Medical Viruses

Reta

Tessema

Ashenef

et al. 2020

International Journal of Microbiology

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Viral infections are causing serious problems in human population worldwide. The recent outbreak of coronavirus disease 2019 caused by SARS-CoV-2 is a perfect example how viral infection could pose a great threat to global public health and economic sectors. Therefore, the first step in combating viral pathogens is to get a timely and accurate diagnosis. Early and accurate detection of the viral presence in patient sample is crucial for appropriate treatment, control, and prevention of epidemics. Here, we summarize some of the molecular and immunological diagnostic approaches available for the detection of viral infections of humans. Molecular diagnostic techniques provide rapid viral detection in patient sample. They are also relatively inexpensive and highly sensitive and specific diagnostic methods. Immunological-based techniques have been extensively utilized for the detection and epidemiological studies of human viral infections. They can detect antiviral antibodies or viral antigens in clinical samples. There are several commercially available molecular and immunological diagnostic kits that facilitate the use of these methods in the majority of clinical laboratories worldwide. In developing countries including Ethiopia where most of viral infections are endemic, exposure to improved or new methods is highly limited as these methods are very costly to use and also require technical skills. Since researchers and clinicians in all corners of the globe are working hard, it is hoped that in the near future, they will develop good quality tests that can be accessible in low-income countries.

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