Study on Apoptosis in Human Deciduous Tooth Pulp Cells

Okai, Yuko; Harada, Kyoko; Ohura, Kiyoshi; Arita, Kenji

doi:10.2485/jhtb.21.413

Cited by 3 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These functions were only detected in the dental pulp of a normal permanent tooth. Moreover, the function of cell death and regulation of apoptosis process, which normally occur as part of the regenerative process and wound healing of dental pulp [ 36 , 37 ], were only identified in normal permanent dental pulp tissue.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth

Lertruangpanya,

Roytrakul,

Surarit

et al. 2024

Clin Oral Invest

View full text Add to dashboard Cite

Objectives To obtain and compare the protein profiles of supernumerary and normal permanent dental pulp tissues. Materials and methods Dental pulp tissues were obtained from supernumerary and normal permanent teeth. Proteins were extracted and analyzed by liquid chromatography-tandem mass spectrometry (LC/MS-MS). Protein identification and quantification from MS data was performed with MaxQuant. Statistical analysis was conducted using Metaboanalyst to identify differentially expressed proteins (DEPs) (P-value < 0.05, fold-change > 2). Gene Ontology enrichment analyses were performed with gProfiler. Results A total of 3,534 proteins were found in normal dental pulp tissue and 1,093 in supernumerary dental pulp tissue, with 174 DEPs between the two groups. This analysis revealed similar functional characteristics in terms of cellular component organization, cell differentiation, developmental process, and response to stimulus, alongside exclusive functions unique to normal permanent dental pulp tissues such as healing, vascular development and cell death. Upon examination of DEPs, these proteins were associated with the processes of wound healing and apoptosis. Conclusions This study provides a comprehensive understanding of the protein profile of dental pulp tissue, including the first such profiling of supernumerary permanent dental pulp. There are functional differences between the proteomic profiles of supernumerary and normal permanent dental pulp tissue, despite certain biological similarities between the two groups. Differences in protein expression were identified, and the identified DEPs were linked to the healing and apoptosis processes. Clinical relevance This discovery enhances our knowledge of supernumerary and normal permanent pulp tissue, and serves as a valuable reference for future studies on supernumerary teeth.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth

Lertruangpanya,

Roytrakul,

Surarit

et al. 2024

Clin Oral Invest

View full text Add to dashboard Cite

show abstract

“…rs2046315 is located on chromosome 8q21.3 and is 870 kb upstream from MMP16 and 560 kb away from the nearest gene RIPK2 (receptor-interacting serine-threonine kinase 2). RIKP2 has no known role in caries etiology, though it has been detected to be involved in apoptosis and is expressed in both deciduous and permanent tooth pulp cells [ 50 ]. Nearby rs10429371, also upstream of MMP16 , showed evidence of association across multiple samples, though it was likewise driven by the COHRA1 white adults.…”

Section: Discussionmentioning

confidence: 99%

Genetic Association ofMMP10,MMP14, andMMP16with Dental Caries

Lewis

Shaffer

Feingold

et al. 2017

International Journal of Dentistry

View full text Add to dashboard Cite

Matrix metalloproteinases (MMPs), which degrade extracellular proteins as part of a variety of physiological processes, and their inhibitors have been implicated in the dental caries process. Here we investigated 28 genetic variants spanning the MMP10, MMP14, and MMP16 genes to detect association with dental caries experience in 13 age- and race-stratified (n = 3,587) samples from 6 parent studies. Analyses were performed separately for each sample, and results were combined across samples by meta-analysis. Two SNPs (rs2046315 and rs10429371) upstream of MMP16 were significantly associated with caries in an individual sample of white adults and via meta-analysis across 8 adult samples after gene-wise adjustment for multiple comparisons. Noteworthy is SNP rs2046315 (p = 8.14 × 10−8) association with caries in white adults. This SNP was originally nominated in a genome-wide-association study (GWAS) of dental caries in a sample of white adults and yielded associations in a subsequent GWAS of surface level caries in white adults as well. Therefore, in our study, we were able to recapture the association between rs2046315 and dental caries in white adults. Although we did not strengthen evidence that MMPs 10, 14, and 16 influence caries risk, MMP16 is still a likely candidate gene to pursue.

show abstract

“…et al, 2016). Both caspases then activate downstream effector Caspases-3 and -7 (Okai et al, 2012;Wu C.C. et al, 2016;Tummers and Green, 2017), leading to cleavage of the host cell DNA, cytoskeletal scaffold protein, and the nuclear membrane (Nicholson and Thornberry, 1997;Chang and Yang, 2000).…”

Section: Contribution Of Caspases To Odontogenesismentioning

confidence: 99%

Role of Cell Death in Cellular Processes During Odontogenesis

Abramyan

Geetha-Loganathan

Šulcová

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The development of a tooth germ in a precise size, shape, and position in the jaw, involves meticulous regulation of cell proliferation and cell death. Apoptosis, as the most common type of programmed cell death during embryonic development, plays a number of key roles during odontogenesis, ranging from the budding of the oral epithelium during tooth initiation, to later tooth germ morphogenesis and removal of enamel knot signaling center. Here, we summarize recent knowledge about the distribution and function of apoptotic cells during odontogenesis in several vertebrate lineages, with a special focus on amniotes (mammals and reptiles). We discuss the regulatory roles that apoptosis plays on various cellular processes during odontogenesis. We also review apoptosis-associated molecular signaling during tooth development, including its relationship with the autophagic pathway. Lastly, we cover apoptotic pathway disruption, and alterations in apoptotic cell distribution in transgenic mouse models. These studies foster a deeper understanding how apoptotic cells affect cellular processes during normal odontogenesis, and how they contribute to dental disorders, which could lead to new avenues of treatment in the future.

show abstract

Study on Apoptosis in Human Deciduous Tooth Pulp Cells

Cited by 3 publications

References 22 publications

Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth

Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth

Genetic Association ofMMP10,MMP14, andMMP16with Dental Caries

Role of Cell Death in Cellular Processes During Odontogenesis

Contact Info

Product

Resources

About