Study of β-lactam resistance in ceftazidime-resistant clinical isolates of Enterobacteriaceae

Bujdáková, Helena; Lausová, A; Jankovičová, Sylvia; Prodinger, Wolfgang M.; Kallová, J; Milosovic, P; Kettner, M.

doi:10.1016/s0924-8579(98)00027-2

Cited by 12 publications

(5 citation statements)

References 25 publications

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“…For example the resistant rate to ciprofloxacin was (40.7%) this result was comparable to the result of Shamm et al( 2001) (20) found in a study that the resistant percentage of E. coli to ciprofloxacin was (39%). Resistance to pipracillin was (85.5%), this result was in agreement with that of Bujdakova et al(1998) (21) who found that (86%) of E. coli isolates resistant to pipracillin , and this may be due to the ability of E. coli to develop resistance to these antimicrobials through the production of β-lactamase enzyme which break the β-lactam ring of pipracillin. Resistance to nitrofurantoin was (2.6%), this result was in agreement with Akyar (2008) (22) who found that the resistant rate of E. coli against nitrofurantoin was (3%).Resistance to trimethoprim/ sulfamethoxazole (SXT) was (43.4%), this result may be attributed to the wide use of (SXT) as empirical therapy for urinary tract infection, however this result was in agreement with Gupta; Hooton and Stamm (2001) (23) who found that the resistance to (SXT) among E. coli isolates from patient with UTIs has increased, with a prevalence of resistance which is reported 30 to 50 percent .…”

Section: -Qualitative Methods (Disc Diffusion Test)supporting

confidence: 90%

Efficacy of Combination of Meropenem with Gentamicin, and Amikacin against Resistant E. coli Isolated from Patients with UTIs: in vitro Study

Khaleq¹,

Abdulkareem²,

Dhahi³

2017

IJPS

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Seventy five E. coli isolates were collected from urine of patients with urinary tract infections in AL-Kadhimia and AL-Yarmook teaching hospitals in Baghdad for a period between 22/11/2009 to 15/3/2010, from these samples twenty five isolates were selected according to their pattern of the highest resistance as these showing multi-drug resistances and tested to specify their minimum inhibitory concentration for (meropenem, gentamicin and amikacin), meropenem was found having the lowest MIC comparing with others. This study also includes in vitro effects of various combinations of three types of antimicrobials (meropenem, gentamicin and amikacin) against twenty five E. coli isolates.Among combinations the combination of meropenem with the other types of antimicrobials showed high synergistic effect when 1/4+1/4 MIC for each antimicrobial were used. While combinations of amikacin with gentamicin in some isolates showed additive effect when 1/2+1/2 MIC for each antimicrobial were used. The plasmid profile for the twenty five E. coli isolates were studied using Pure Yeild â„¢ plasmid Miniprep system- Cat.# A1220 â€“ Promega- USA. In order to determined the presence of plasmid for antimicrobials resistance.

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Section: -Qualitative Methods (Disc Diffusion Test)supporting

confidence: 90%

Efficacy of Combination of Meropenem with Gentamicin, and Amikacin against Resistant E. coli Isolated from Patients with UTIs: in vitro Study

Khaleq¹,

Abdulkareem²,

Dhahi³

2017

IJPS

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“…All bacterial pathogens showed a variable resistance to beta (β) -lactam antibiotics such as, Ampicillin, Amoxicillin and Penicillin G. These agreements, with many earlier studies, reported a high degree of these drug resistances [41]. The Gram negative bacilli also were resistant to Cephalothin, Rifampicin and Erythromycin that reported the high prevalence of resistance to the commonly used antibiotics such as Ampicillin and Cephalothin [42]. Staphylococcus aureus was resistance to Ceftriaxone as (71.4%) and Cefotaxime as (57.1)%, these results are supported by the other work [43,44], Ciprofloxacin showed a strong activity against of all bacterial isolated and were very active against E.coli and Proteus mirabilis, however it has a less activity against Staphylococcus aureus , K. pneumonia and Pseudomonas aeruginosa.…”

Section: Discussionsupporting

confidence: 65%

Bacterial Pathogens of Patients with Urinary Tract Infections and Antibiotics Susceptibility in Taiz Governorate-Yemen

alganady¹

2019

MJS

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One of the most prevalent bacterial infections are urinary tract infections (UTIs), which affect any age of individuals and they appear higher in women than men, the major problem regarding UTIs are the difficult of antibiotics treatment, due to bacterial pathogens resistance. So, the current study aimed to detect the types of bacterial pathogens and their antibiotics susceptibility of UTIs in Taiz Governorate – Yemen. In this cohort study, one hundred and ten (110) mid-stream urine samples were collected from a symptomatic UTIs patient, the type of bacterial pathogens was identified by using standard bacteriological media and biochemical tests and determine the antibiotics sensitivity to these bacteria using disk diffusion tests. Out of one hundred and ten of urine samples:: 65 of patients were females and 45 were males and the bacterial growth in these patients were sixty-four [46(58.2%)] : (33.7%) were females and (24.5%) were males. Our findings showed that the types of bacterial pathogens include: Escherichia coli (32.7%), Klebsiellapneumoniae (9.1%), Staphylococcus aureus (6.4%), Proteus mirabilis (5.5%) and Pseudomonas aeruginosa (4.5%). The majority (57.9%) of the isolates were from female. The prevalence of UTIs is high among patients with age between 16-30 years with the incident rate 20.9 %, age groups 1-15 years have the lower incidence rate 5.5%. UTIs were more prevalent among the rural area population, there were 50% than the population of the city center, there were 8.2% and the rate of infections were more prevalent among illiterate (no read and write) patients with incidence rate 44.5% , compared to literate (read and write) patients 13.6%. Also, our results showed that the sensitivity test against bacterial pathogens as All bacterial isolates was more sensitive to Ciprofloxacin, followed by Amikacin, Gentamycin , Cefotaxime (except Staphylococcus aureus Cefotaxime (42.9%) and Ceftriaxone (except Staphylococcus aureus) (28.6%). Staphylococcus aureus isolated also were sensitive to Erythromycin, Rifampicin, and Cephalothin. Finally, all isolates showed very high rate of resistance against Amoxicillin, ampicillin and penicillin G.

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“…Despite the fact that several antibiotics are available commercially for the treatment of both Gram-positive and Gram-negative bacteria, their extensive use has led in developing resistance in these pathogens; antibiotic resistance constitutes a significant challenge in global public health [3]. For instant, bacteria have reportedly acquired a novel form of β-lactamase enzyme that results in conferring resistance to antibiotics containing β-lactam ring such as cefotaxime [4][5][6]. Changing the structure of antibiotics' β-lactam moiety [7,8] and synthesizing new antibiotics with higher potencies [9] are two important strategies used for overcoming bacterial resistance.…”

Section: Introductionmentioning

confidence: 99%

Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity

et al. 2022

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Cefotaxime (CTX) is a third-generation cephalosporin antibiotic with broad-spectrum activity against Gram negative and Gram positive bacteria. However, like other third-generation cephalosporin antibiotics, its efficacy is declining due to the increased prevalence of multidrug-resistant (MDR) pathogens. Recent advances in nanotechnology have been projected as a practical approach to combat MDR microorganisms. Therefore, in the current study, gold nanoparticles (AuNPs) were prepared using cefotaxime sodium, which acted as a reducing and capping agent, besides having well-established antibacterial activity. The synthesized cefotaxime-loaded gold nanoparticles (C-AuNPs) were characterized by UV-Visible spectroscopy, FTIR, TEM and DLS. In addition, the in vitro antibacterial activity of C-AuNPs was assessed against both Gram-positive and Gram-negative bacteria. UV-Visible spectroscopy verified the formation of C-AuNPs, while TEM and DLS verified their nano-size. In addition, CTX loading onto AuNPs was confirmed by FTIR. Furthermore, the colloidal stability of the synthesized C-AuNPs was ascribed to the higher net negative surface charge of C-AuNPs. Most importantly, the synthesized C-AuNPs showed superior antibacterial activity and lower minimum inhibitory concentration (MIC) values against Gram-negative (Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa) and gram-positive (Staphylococcus aureus) bacteria, compared with pure CTX. Collectively, CTX was successfully adopted, as reducing and capping agent, to synthesize stable, nano-sized spherical C-AuNPs. Furthermore, loading CTX onto AuNPs could efficiently restore and/or boost the antibacterial activity of CTX against resistant Gram-negative and Gram-positive bacteria.

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Study of β-lactam resistance in ceftazidime-resistant clinical isolates of Enterobacteriaceae

Cited by 12 publications

References 25 publications

Efficacy of Combination of Meropenem with Gentamicin, and Amikacin against Resistant E. coli Isolated from Patients with UTIs: in vitro Study

Efficacy of Combination of Meropenem with Gentamicin, and Amikacin against Resistant E. coli Isolated from Patients with UTIs: in vitro Study

Bacterial Pathogens of Patients with Urinary Tract Infections and Antibiotics Susceptibility in Taiz Governorate-Yemen

Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity

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