Study of transgen functionality in T2 biotechnological plants of winter wheat on the sign of osmoresistance

Abstract

Aim. To investigate the functionality of the transgene in the seed generation (T2) of genetically modified winter wheat plants (Triticum aestivum L.) in terms of productivity in conditions of normal and insufficient water supply. Methods. Determination of yield structure and protein content. Results. The indicators of productivity of control and T2 biotechnological plants under normal growing conditions and water deficit are analyzed. The quantitative composition of protein in the conditions before and after osmotic stress and in the period of rehydration was determined. Conclusions. It is shown that under the action of water deficit there is a decrease in productivity for all studied plants. In this case, genetically modified plants had an advantage in the main elements of the crop structure over the original form under both analyzed growing conditions. It was found that under normal conditions of hydration, the amount of protein in the studied plants did not differ significantly. An increase in its content in control plants under the action of osmotic stress was recorded, which may indicate the synthesis of stress response proteins. Decreased protein content during rehydration may indicate the passage of normal metabolic processes.
 Keywords: Triticum aestivum L., biotechnological plants, proline dehydrogenase gene, structural analysis of yield, osmostability.

Physiological and biochemical characteristics of genetically modified winter wheat

“…Об'єктом дослідження було насіннєве покоління Т2 генетично-змінених рослин пшениці озимої генотипів УК 322/17 та УК 209h, які були отримані шляхом Agrobacterium-опосередкованої трансформації in planta з використанням штаму A. tumefaciens AGLO, що несе векторну конструкцію pBi2E [8].…”

Agrobacterium-mediated transformation – method of genetic modification of Triticum aestivum L. plants

“…Вміст вільного L-проліну визначали за модифікованою методикою Чинарда [9]. Відбір зразків для структурного аналізу проводили у фазі повної стиглості насіння [10]. Експериментально отримані дані обробляли методами математичної статистики [11].…”