Study of Three Sesquiterpene Lactones from<i>Tithonia diversifolia</i>on their Anti-Inflammatory Activity Using the Transcription Factor NF-κB and Enzymes of the Arachidonic Acid Pathway as Targets

Rüngeler, Peter; Lyß, Guido; Castro, Vı́ctor; Mora, Gerardo; Pahl, Heike L.; Merfort, Irmgard

doi:10.1055/s-2006-957527

Cited by 83 publications

(55 citation statements)

References 8 publications

(10 reference statements)

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“…P65 activity and MMP-9 secretion was reduced by a factor of 8 ϫ 2 M helenalin with no significant cell death. Sesquiterpene lactones are the active component in traditional antiinflammatory remedies using plants from the genus Arnica (27,48).…”

Section: Discussionmentioning

confidence: 99%

Monocyte-Astrocyte Networks Regulate Matrix Metalloproteinase Gene Expression and Secretion in Central Nervous System Tuberculosis In Vitro and In Vivo

Harris

Nuttall

Elkington

et al. 2007

The Journal of Immunology

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CNS tuberculosis (CNS-TB) is the most deadly form of tuberculous disease accounting for 10% of clinical cases. CNS-TB is characterized by extensive tissue destruction, in which matrix metalloproteinases (MMPs) may play a critical role. We investigated the hypothesis that Mycobacterium tuberculosis activates monocyte-astrocyte networks increasing the activity of key MMPs. We examined the expression of all human MMPs and the tissue inhibitors of metalloproteinases (TIMPs) in human astrocytes stimulated by conditioned medium from M. tuberculosis-infected monocytes (CoMTB). Real-time RT-PCR showed that gene expression of MMP-1, -2, -3, -7, and -9 was increased (p < 0.05). MMP-9 secretion was significantly up-regulated at 24 h and increased over 120 h (p < 0.01). MMP-1, -3, and -7 secretion was not detected. Secretion of MMP-2 was constitutive and unaffected by CoMTB. Astrocyte gene expression and secretion of TIMP-1 was not affected by CoMTB although TIMP-2 secretion increased 3-fold at 120 h. Immunohistochemical analysis of human brain biopsies confirmed that astrocyte MMP-9 secretion is a predominant feature in CNS-TB in vivo. Dexamethasone inhibited astrocyte MMP-9, but not TIMP-1/2 secretion in response to CoMTB. CoMTB stimulated the nuclear translocation of NF-κB, inducing a 6-fold increase in nuclear p65 and a 2-fold increase in nuclear p50. This was associated with degradation of IκBα and β within 30 min, persisting for 24 h. In summary, networks active between monocytes and astrocytes regulate MMP-9 activity in tuberculosis and astrocytes are a major source of MMP-9 in CNS-TB. Astrocytes may contribute to a matrix degrading environment within the CNS and subsequent morbidity and mortality.

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Section: Discussionmentioning

confidence: 99%

Monocyte-Astrocyte Networks Regulate Matrix Metalloproteinase Gene Expression and Secretion in Central Nervous System Tuberculosis In Vitro and In Vivo

Harris

Nuttall

Elkington

et al. 2007

The Journal of Immunology

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“…Its leaves are greyish green, the stems are hallow and slightly ridged. T. diversifolia performs several functions such as antibacterial (Victor, 2004), antibacterial (Kareru et al, 2010;Rungeler, 1998), anti-inflammatory (Obafemi et al, 2006), antimicrobial (Liasu et al, 2008), antidiabetic (Toshihiro et al, 2005) and antioxidant (Robson et al, 2014). According to Akinola et al (1999); Odedire and Oloidi (2014) the plants can be cultivated by resource poor farmers who could manipulate planting density to achieve maximum yield.…”

Section: Introductionmentioning

confidence: 99%

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2018

GJAS

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This experiment was carried out to investigate the effect of feeding different levels of Azolla pinnata, Polyalthia longifolia, Tithonia diversifolia, Moringa olifera, Azadirachta indica leaf meal infusion (PMNWA) as an organic supplement on the performance and nutrient retention of growing grass cutters. Experimental parameters covered growth performance and nutrient retention. Twenty, three weeks growing male grass cutters were randomly divided into five treatments groups with four replicates, each of one grass cutter in a Completely Randomized Design (CRD). Group 1 was fed basal diet without PMNWA, Group 2, 3, 4 and 5 were fed basal diets supplemented with PMNWA at levels of 5.00%, 10.0%, 15.0% and 20.0% respectively. The basal diet was formulated to meet the nutritional requirements of growing grass cutters according to Wogar (2011), the experiment lasted for 60 days and the results obtained showed that there were significant at P<0.05 differences among all treatments in the values of final live weight and feed conversion ratio . Results for feed intake, daily water intake and nutrient retention (protein, fibre and fat) were not significantly (P>0.05) affected with the inclusion of PMNWA. No mortality was recorded throughout the experimental period. Results obtained from this study showed that PMNWA is a good quality test materials with high contents of micro-nutrients which can improve the stability, palatability and shelf life of feed and its inclusion at 20.0% level does not have any deleterious effect on the general performance and health status of grass cutters.

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“…Gray, Asteraceae) [2]. It shows cytotoxic [19], anti-inflammatory [33], and antifeedant activities [2], and thus is a promising compound for further research into its mechanisms of action in different targets. T. diversifolia, the source of 1, is used in folk medicine in many countries [8,14] and has anti-inflammatory and antimalarial properties [8].…”

Section: Introductionmentioning

confidence: 99%

Microbial transformation of the sesquiterpene lactone tagitinin C by the fungus Aspergillus terreus

Rocha

Pupo

Antonucci³

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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The biotransformation of the sesquiterpene lactone tagitinin C by the fungus Aspergillus terreus MT 5.3 yielded a rare derivative that was elucidated by spectrometric methods. The fungus led to the formation of a different product through an unusual epoxidation reaction between C4 and C5, formation of a C3,C10 ether bridge, and a methoxylation of the C1 of tagitinin C. The chemical structure of the product, namely 1b-methoxy-3a-hydroxy-3,10b-4,5a-diepoxy-8b-isobutyroyloxygermacr-11(13)-en6a,12-olide, is the same as that of a derivative that was recently isolated from the flowers of a Brazilian population of Mexican sunflower (Tithonia diversifolia), which is the source of the substrate tagitinin C. The in vitro cytotoxic activity of the substrate and the biotransformed product were evaluated in HL-60 cells using an MTT assay, and both compounds were found to be cytotoxic. We show that soil fungi may be useful in the biotransformation of sesquiterpene lactones, thereby leading to unusual changes in their chemical structures that may preserve or alter their biological activities, and may also mimic plant biosynthetic pathways for production of secondary metabolites.

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Study of Three Sesquiterpene Lactones fromTithonia diversifoliaon their Anti-Inflammatory Activity Using the Transcription Factor NF-κB and Enzymes of the Arachidonic Acid Pathway as Targets

Cited by 83 publications

References 8 publications

Monocyte-Astrocyte Networks Regulate Matrix Metalloproteinase Gene Expression and Secretion in Central Nervous System Tuberculosis In Vitro and In Vivo

Monocyte-Astrocyte Networks Regulate Matrix Metalloproteinase Gene Expression and Secretion in Central Nervous System Tuberculosis In Vitro and In Vivo

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Microbial transformation of the sesquiterpene lactone tagitinin C by the fungus Aspergillus terreus

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