Study of the Tip Surface Morphology of Glass Micropipettes and Its Effects on Giga-Seal Formation

Malboubi, Majid; Gu, Yuchun; Jiang, Kyle

doi:10.1007/978-90-481-8776-8_52

Cited by 5 publications

(5 citation statements)

References 18 publications

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“…Optical and electron microscope images of patches show that membrane and pipette are in close contact, but they do not show the surface topography involved in seal formation [ 20 , 21 ]. Surface roughness of glass micropipettes is reported in the literature to play an important role in giga-seal formation [ 11 - 13 , 22 , 23 ]. Normally, pipettes are fire polished before experiments to make rough tips smoother.…”

Section: Resultsmentioning

confidence: 99%

“…Recent development in microengineering and nanosciences has found many applications of micro/nanopipettes, such as generating microdroplets [ 8 ], single-molecule fluorescence tracking [ 2 ], creating nanoscale features by nanolithography and nanowriting methods [ 9 ], and nanosensing in scanning probe microscopy [ 10 ]. Although there are many studies in the literature on the shapes and geometries of pipettes [ 1 , 6 , 11 - 15 ], there are no reports about numerical analysis on the effect of pulling parameters on surface roughness properties of glass micropipettes. This information is important in applications which require direct contact of pipette and samples.…”

Section: Introductionmentioning

confidence: 99%

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Surface properties of glass micropipettes and their effect on biological studies

Malboubi

Jiang

2011

Nanoscale Res Lett

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In this paper, an investigation on surface properties of glass micropipettes and their effect on biological applications is reported. Pipettes were pulled under different pulling conditions and the effect of each pulling parameter was analyzed. SEM stereoscopic technique was used to reveal the surface roughness properties of pipette tip and pipette inner wall in 3D. More than 20 pipettes were reconstructed. Pipette heads were split open using focused ion beam (FIB) milling for access to the inner walls. It is found that surface roughness parameters are strongly related on the tip size. Bigger pipettes have higher average surface roughness and lower developed interfacial area ratio. Furthermore, the autocorrelation of roughness model of the inner surface shows that the inner surface does not have any tendency of orientation and is not affected by pulling direction. To investigate the effect of surface roughness properties on biological applications, patch-clamping tests were carried out by conventional and FIB-polished pipettes. The results of the experiments show that polished pipettes make significantly better seals. The results of this work are of important reference value for achieving pipettes with desired surface properties and can be used to explain biological phenomenon such as giga-seal formation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface properties of glass micropipettes and their effect on biological studies

Malboubi

Jiang

2011

Nanoscale Res Lett

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“…The experimental equipment setup consisted of an Axon multiclamp 700B microelectrode amplifier (Axon Instruments, Union City, CA), Flaming/Brown No surface roughness could be identified after milling, so the surface roughness should be smaller than the resolution of the SEM image, which is 4.5 nm [2] micropipette puller (Model P-97, Sutter Instruments) and glass micropipettes (BF150-86-10, Sutter Instruments). The puller machine was set to produce pipettes with a tip size of approximately 1.5 μm.…”

Section: Patch Clamping Experimentsmentioning

confidence: 99%

Effect of Roughness on Gigaseal Formation

Malboubi

Jiang

2013

Gigaseal Formation in Patch Clamping

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“…The 3D reconstruction methods of surface topography, necessary to obtain roughness parameters, can be divided into two principal categories: 1) Stereoscopy, in which a SEM image stereo pair is used. The stereo pair can be obtained by deflecting the electron beam, but it is generally implemented by tilting the specimen stage (Lane, 1969(Lane, , 1972Stampfl et al, 1996;Davies and Randle, 2001;Huang et al, 2004;Bonetto et al, 2006;Ponz et al, 2006;Jahnisch and Fatikow, 2007;Marinello et al, 2008;Ostadi et al, 2009;Malboubi et al,2009;Fatikow et al, 2009;Azevedo and Marques, 2010;Chen et al, 2010;Ostadi et al, 2010). Both, observation of 3D images and measurement of 3D height data are possible.…”

Section: ) Introductionmentioning

confidence: 99%

Uncertainty studies of topographical measurements on steel surface corrosion by 3D scanning electron microscopy

Kang

Pereda

Canafoglia

et al. 2012

Micron

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Pitting corrosion is a damage mechanism quite serious and dangerous in both carbon steel boiler tubes for power plants which are vital to most industries and stainless steels for orthopedic human implants whose demand, due to the increase of life expectation and rate of traffic accidents, has sharply increased. Reliable methods to characterize this kind of damage are becoming increasingly necessary, when trying to evaluate the advance of damage and to establish the best procedures for component inspection in order to determine remaining lives and failure mitigation. A study about the uncertainties on the topographies of corrosion pits from 3D SEM images, obtained at low magnifications (where errors are greater) and different stage tilt angles were carried out using an in-house software previously developed. Additionally, measurements of pit depths on biomaterial surfaces, subjected to two different surface treatments on stainless steels, were carried out. The different depth distributions observed were in agreement with electrochemical measurements.

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Study of the Tip Surface Morphology of Glass Micropipettes and Its Effects on Giga-Seal Formation

Cited by 5 publications

References 18 publications

Surface properties of glass micropipettes and their effect on biological studies

Surface properties of glass micropipettes and their effect on biological studies

Effect of Roughness on Gigaseal Formation

Uncertainty studies of topographical measurements on steel surface corrosion by 3D scanning electron microscopy

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