Study of the Therapeutic Efficiency of Transduced Olfactory Ensheathing Cells in Spinal Cord Cysts

Prospects for the use of olfactory mucosa cells in bioprinting for the treatment of spinal cord injuries

“…In the authors’ laboratory, for the first time, the therapeutic effect of OECs was shown in the treatment of posttraumatic spinal cord cysts. The transplantation of OECs contributed to a decrease in the volume of cysts and restoration of limb motor activity[ 32 , 33 ].…”

“…Further in vitro studies showed that BDNF expressed by the OECs contributed to the transplant-mediated axon growth[ 47 ]. For the first time, the authors of this paper have demonstrated that the combined introduction of OECs and exogenous NT-3 to the modelled cysts in the rat spinal cord improved the motor activity of the hind limbs and reduced the cyst size[ 32 ]. The use of NT-3 improves the effects obtained from cell therapy and the data are presented in Figure 1 [ 48 ].…”

“…It was shown that transplantation of OECs obtained from olfactory mucosa, transduced by an adenoviral vector encoding a mature form of BDNF, into a post-traumatic cyst could have a therapeutic effect. This is observed not only due to increased secretion of neurotrophin but also due to the regenerative potential of the cells themselves[ 32 ]. Other authors showed the effectiveness of OECs obtained from olfactory mucosa transduced with adenoviral vector transcoding NT-3.…”

Spinal cord injury: Olfactory ensheathing cell‐based therapeutic strategies

“…Genetic engineering of OECs opens the possibility of expressing additional target genes that can be modified to produce specific gene products to improve the spinal microenvironment and contribute to SCI therapy. For example, OECs genetically modified to secrete BDNF can show better effects than common OEC post‐transplantation (Stepanova et al., 2022). Based on this, nanoparticle technology has been utilized to deliver gene‐edited OECs to reduce the side effects of traditional transfection methods (Delaney et al., 2017).…”

“…Neurotrophins can stimulate favorable behaviors in OECs, such as proliferation, migration, phagocytosis, and interactions with other cells (Wright et al., 2018). Therefore, GDNF is a likely candidate for combined treatment with OEC transplantation because it aids the migration and integration of OECs into the SCI site (Stepanova et al., 2022).…”