Study of the soil isolates for antimicrobial activity

Srividya, A. R.; Saritha, G; Suresh, B

doi:10.4103/0250-474x.49132

Cited by 9 publications

(8 citation statements)

References 8 publications

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“…The broth containing bacterial culture was centrifuged at 9000 g for 15 min at 4 °C to remove the biomass and cell debris. The supernatant was extracted with ethyl acetate 1:1 ( v / v ) [41]. The organic phase was separated and condensed in a rotary evaporated with vacuum (BT 351 Rotary Evaporator, BIOTHEC, Germany).…”

Section: Methodsmentioning

confidence: 99%

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

et al. 2019

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Microorganisms thrive in extreme environments and are known for synthesizing valuable metabolites. Salt-loving microorganisms can flourish in saline environments which inhibit the growth of other microbial life, and they possess the potential to produce stable and novel biomolecules for the use in biotechnological applications, including anticancer compounds. Sambhar Lake is the largest inland soda lake in India and is an appropriate habitat for halophilic bacterial and archaeal strains in terms of diversity and potential production of bioactive compounds. In the present study, a moderately halo-alkaliphilic bacterial strain C12A1 was isolated from Sambhar Lake, located in Rajasthan, India. C12A1 was gram-positive, motile, rod-shaped, formed oval endospores, produced carotenoids, and exhibited optimal growth at 37 °C in 10–15% NaCl (pH 8). C12A1 was found to be able to hydrolyze skimmed milk, gelatin, and Tween 80 but unable to hydrolyze starch and carboxymethylcellulose. C12A1 showed 98.87% and 98.50% identity in 16S rRNA gene sequence to P. halophilus and P. salipiscarius, respectively. Nevertheless, C12A1 was clustered within the clade consisting of P. salipiscarius strains, but it showed a distinct lineage. Thus, C12A1 was designated as Piscibacillus sp. Cell proliferation assay results showed that C12A1 broth extract (BEP) decreased cell viability in breast cancer MDA-MB-231 cells, which was confirmed by the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Induction of cell toxicity was visualized by microscopy. Reverse Transcriptase PCR (RT-PCR) analysis demonstrated that BEP inhibited the expression of proliferative B-cell lymphoma-extra large (Bcl-xL) and cell cycle marker Cyclin-dependent kinase 2 (CDK2) at transcript levels. Similarly, cell migration and colony formation along with mesenchymal marker vimentin and stem cell marker BMI transcripts were found to be inhibited when cells were treated with the BEP. The anti-breast cancer potential of C12A1 indicates that microorganisms inhabiting saline-alkaline habitats, with Piscibacillus sp. in particular, are a promising source for discovery of novel bioactive substances.

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Section: Methodsmentioning

confidence: 99%

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

et al. 2019

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“…Alkaloids,flavonoids, phytosterols, glycosides of medical importance are present in popular medicinal plants of families like papavaraceae, solanaceae, acanthaceae, apocyanaceae etc,and many reported antibacterial activity of different plants [12][13][14][15][16][17][18][19][20][21][22][23][24] .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2012

IJPSR

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Present study reports phytochemical screening and the inhibitory activity of crude extracts of Cassia tora and Cassia sophera (fam: caesalpinaceae) on some human pathogenic bacteria and fungi. The selected plants are edible and used by local herbal medical practitioners to treat various skin infections and respiratory problems. Leaf and flower extracts were prepared with different solvents and tested for the presence of primary and secondary metabolites which are pharmacologically active compounds. Acetone and Aqueous extracts revealed the presence of more constituents including glycosides, phenols, flavonoids alkaloids etc. As many of the active ingredients in chemically manufactured drugs were originally derived from plant compounds, extracts are also used to screen the antimicrobial activity. The results showed very high inhibitory activity against skin pathogens like Staphylococcus and Candida albicans. The zone of inhibition was determined utilizing the well diffusion method. For comparison chloramphenicol and Fluconazole were used as standard reference. Among bacteria used gram positive bacteria and fungus Candida albicans were most inhibited. Flower and leaf extracts showed different activity, revealing differential solubility of phytochemicals. The present study correlates medicinal properties of C. tora and C. sophera and their use in folklore medicine.

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“…Based on several experimental works, researchers [89] have collected dozens of soil bacteria genera, including eubacteria and actinomycetes, which have different and powerful abilities to produce antibiotics. In India [90], screening was performed for potent antimicrobial compounds and at least one strain with antagonistic abilities against many test microorganisms was isolated. A study in Nigeria [91] is of interest, because genetic techniques were used to show that microorganisms can synthesize useful organic compounds such as antibiotics during their activity with soil contaminants, although only a limited number of the investigated Bacilli species exhibited antibacterial activity.…”

Section: Studies Of Biological Soil Crusts (Bsc)mentioning

confidence: 99%

Biological Soil Crusts and Extremophiles Adjacent to Native Plants at Sabkhas and Rawdahs, Qatar: The Possible Roles

Al‐Thani¹,

Yasseen²

2018

FEM

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Several studies have investigated the biology of native plants (halophytes and xerophytes), biological soil crusts (BSC), as well as the associated and adjacent microorganisms. These studies concentrated on their eco-physiological aspects as well as the possible roles they might play in various life activities and industries. This article presents the mechanisms adopted by these living organisms to cope with the harsh environment, and the possible roles microorganisms might play to support native plants, some important examples from the flora of Qatar are presented to show these mechanisms. Special attention has been paid to the senescence and death of leaves or whole plants, since this strategy has been considered as an important option for escaping extreme environmental conditions. Discussion about solute accumulation (organic and inorganic) in all native living organisms in general and in microorganisms in particular, could result to further investigation to clarify their roles in such an ecosystem. Some case studies have been reported to discuss the ecological aspects at two main types of habitats in Qatar; Rawdahs and Sabkhas were chosen to show how native living organisms adapt in these habitats. This study discussed the general identification of BSC and microorganisms, as well as their possible roles in the soil biota. The possible methods and mechanisms exhibited by extremophiles to support native plants in coping with saline and arid lands have also been reported in this article. This study also discussed the recent outlook about the origin of metabolic activities in these living organisms to support the growth of native plants under stress conditions.

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Study of the soil isolates for antimicrobial activity

Cited by 9 publications

References 8 publications

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

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Biological Soil Crusts and Extremophiles Adjacent to Native Plants at Sabkhas and Rawdahs, Qatar: The Possible Roles

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