Study of the Relationship between Polymorphisms in the IL-8 Gene Promoter Region and Coccidiosis Resistance Index in Jinghai Yellow Chickens

Abstract

Interleukin 8 (IL-8) participates in the immune response and has the function of inducing neutrophils to release lysosomal enzymes and eliminate pathogens. This study was to investigate the effect of single nucleotide mutations in the IL-8 gene promoter region on the coccidiosis resistance index. In this study, 180 infected Eimeria tenella (E. tenella) Jinghai yellow chickens were used as experimental samples. DNA sequencing technology was used to detect single nucleotide polymorphisms (SNPs) in the IL-8 gene promoter region. The association between these SNPs and coccidiosis resistance indexes (including superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX), catalase (CAT), nitric oxide (NO), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), IL-8, and interferon-γ (IFN-γ)) were analyzed. Three SNPs (T-550C, G-398T, and T-360C) were detected. Significant associations were found between each genotype at the T-550C site with NO (p-value = 0.006) and IL-8 (p-value = 0.034) indexes. Significant associations were found between each genotype at the G-398T site with SOD (p-value = 0.042), CAT (p-value = 0.049), NO (p-value = 0.008), and IL-2 (p-value = 0.044) indexes. Significant associations were found between each genotype at the T-360C site with SOD (p-value = 0.007), NO (p-value = 0.046), IL-2 (p-value = 0.041), IL-8 (p-value = 0.039), and IFN-γ (p-value = 0.042) indexes. Haplotype analysis showed that multiple indexes of the H1H3 haplotype combination were significantly higher than other haplotype combinations. Therefore, mutation of the IL-8 gene promoter region has a significant regulatory effect on the coccidiosis resistance index, with a change in transcription factor binding potentially altering IL-8 gene expression, thereby further affecting the IL-8 level in plasma. However, the specific mechanism needs further study.

Comprehensive Analyses of circRNA Expression Profiles and Function Prediction in Chicken Cecums After Eimeria tenella Infection

“…Interleukin-8 (IL-8), known as chemokine CX-CL8 (CXCL8), is a proinflammatory factor closely related to the occurrence and development of various inflammatory diseases. IL-8 exerts various biological functions by binding to the receptors CXCR1 and CXCR2, including pro-inflammatory cell chemotaxis, inducing neutrophils to release lysosomal enzymes to eliminate pathogens ( Coussens and Werb, 2002 ), activating immune responses and promoting infected tissue healing ( Wang et al., 2020 ). As a G-protein-coupled protease-activated receptor, F2RL2 plays an important role in the coagulation cascade ( Kaufmann and Hollenberg, 2012 ).…”

Immunohistochemical Investigation of Lipid Peroxidation in Renal Coccidiosis of Geese

“…Parallel to previous studies, in the histopathological examination of the kidneys, we detected different parasitic forms of E.truncata in the renal tubular epithelium (Klimeš, 1963;Entzeroth et al, 1981;Gajadhar et al, 1982;Gajadhar et al, 1986), severe degeneration and necrosis (Oksanen, 1994;Arslan et al, 2002) and inflammatory infiltration in which the majority of mononuclear cells (Montgomery, 1978;Tuggle and Crites, 1984) were formed. Findings such as hepatitis, enteritis, and pulmonary aspergillosis, which we thought to be caused by avian coccidiosis increasing predisposition to other disease factors, were among the other important histopathological changes we found (Liu et al, 2018;Wang et al, 2020).…”

“…In this study, in accordance with the literature data (Entzeroth et al, 1981;Tuggle and Crites, 1984;Dumanlı and Aktaş, 2015) the mean age range of the geese with renal coccidiosis was 10 weeks. Various clinical symptoms such as diarrhea, depression, anorexia, and emaciation have been reported in previous studies (Arslan et al, 2002;Dai et al, 2005;Song et al, 2017;Liu et al, 2018;Wang et al, 2020) in geese with renal coccidiosis. In our study, in addition to these clinical symptoms, we identified different clinical symptoms such as respiratory distress, tremor, inability to get up, balance disorders, rotational movement around their axis, and wheezing.…”

“…One of the most important problems encountered in goose breeding is the fight against infectious diseases and protection (Sarı and Saatcı, 2020). One of the most important of these infectious diseases is coccidiosis infection caused by parasites related to Eimeria lineage, which causes significant losses in goose breeding (Liu et al, 2018;Wang et al, 2020).…”

Changes of oxidant-antioxidant parameters in small intestines from rabbits infected with E. intestinalis and E. magna

“…According to the recent literature, the altered activities of some antioxidant substances and antioxidant enzymes in parasitic diseases and coccidiosis have been reported (Matés et al, 1999;Georgieva et al, 2006;Wang et al, 2020), but there was lack of information on the changes of oxidative stress-related parameters in the intestinal tissues of rabbits infected with E. intestinalis and E. magna. Therefore, the present study focused on measuring MDA levels and antioxidant enzyme activities of SOD, CAT, GSH-Px and T-AOC in the intestinal tissues of rabbits infected with E. intestinalis and E. magna.…”