Study of the reciprocal relationship of the exoantigens ofClostridium perfringens type A in the culture medium and the bacterial cells during cultivation

Shemanova, G. F.; Gorshkova, V. I.; Borisova, O. K.; Shakhanina, K. L.

doi:10.1007/bf02867044

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…was also shown (Shemanova, Gorshkova, Borisova & Shakhanina, 1970) that the rapid decline of phospholipase C activity in the supernatant fluid coincided with the appearance of maximum proteolytic activity. However, in this study very low amounts of proteolytic activity were detected by comparison with Shemanova et al (1970), which makes it less probable that proteolytic: degradation was responsible for the rapid loss of phospholipase C activity.…”

Section: Mollby C Smyth a N D T W A D S T R O Mmentioning

confidence: 80%

Formation of Phospholipase C and Theta-haemolysin in Pre-reduced Media in Batch and Continuous Culture of Clostridium perfringens Type A

Nord

Möllby

Smyth

et al. 1974

Journal of General Microbiology

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S U M M A R YClostridium perfringens type A ( A T C C I~ 124) was grown in bottles (static culture) and in fermenters (batch and continuous culture) in a proteose peptone medium supplemented with phosphate, cysteine, vitamins and different carbohydrates. The lag phase was shorter and the growth rate higher when the medium was pre-reduced. The final yields of bacteria, phospholipase C, and theta-haemolysin were also significantly higher in the pre-reduced medium. The optimum pH for maximum bacterial yield was 7.0 and the optimum temperature for growth was 37 "C. The formation of phospholipase C was optimum between pH 6.0 and 7-0 and for thetahaemolysin between pH 7.0 and 7.5. The optimum temperature for synthesis of both phospholipase C and theta-haemolysin was 37 "C. Culture under controlled conditions gave more reproducible production of these two proteins than experiments in bottles. The cultures reached the stationary phase before phospholipase C activity started to decline, while theta-haemolysin activity was stable. The strain was also grown in continuous cuIture at a dilution rate of 0.4 h-I, at pH 7-0 and 37 "C, with a yield of I ~2 mg dry wt/ml in the complex medium supplemented with glucose (5 g/l). After the continuous feed was started the activity of phospholipase C declined very rapidly to zero, while theta-haemolysin continued to be produced at a constant level. Thus continuous culture offered no benefit over batch culture for reproducible laboratory-scale production of phospholipase C.

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Section: Mollby C Smyth a N D T W A D S T R O Mmentioning

confidence: 80%

Formation of Phospholipase C and Theta-haemolysin in Pre-reduced Media in Batch and Continuous Culture of Clostridium perfringens Type A

Nord

Möllby

Smyth

et al. 1974

Journal of General Microbiology

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Nutrition and Toxin Production by Clostridium Bifermentans in Relation to Sporulation

Cox

Holland

1977

Spore Research 1973

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Clostridium perfringens CELLS AND PHOSPHOLIPASE C ACTIVITY AT CONSTANT AND LINEARLY RISING TEMPERATURES

Foegeding

Busta

1980

Journal of Food Science

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Growth and phospholipase C production by Clostridium perfringens ATCC 13124 were evaluated in autoclaved ground beef at four constant temperatures and at three rising temperature rates representative of long time low temperature (LTLT) food processing. The total activity of phospholipase C per unit volume, determined by the hemolysin indicator (HI) method, correlated directly to the CFU/g measured on Tryptose Sulfite Cycloserine agar during constant temperature growth and under rising temperature conditions that result in growth and inactivation. Inactivation kinetics of C. perfringens and phospholipase C were compared at temperatures encountered (luring exposure to rising temperatures. Inactivation kinetics of C. perfringens ATCC 13124 and phospholipase C produced by strain ATCC 13124 in autoclaved ground beef are essentially identical. The HI method appears applicable for estimating viable C. perfringens in LTLT processed products.

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Study of the reciprocal relationship of the exoantigens ofClostridium perfringens type A in the culture medium and the bacterial cells during cultivation

Cited by 3 publications

References 8 publications

Formation of Phospholipase C and Theta-haemolysin in Pre-reduced Media in Batch and Continuous Culture of Clostridium perfringens Type A

Formation of Phospholipase C and Theta-haemolysin in Pre-reduced Media in Batch and Continuous Culture of Clostridium perfringens Type A

Nutrition and Toxin Production by Clostridium Bifermentans in Relation to Sporulation

Clostridium perfringens CELLS AND PHOSPHOLIPASE C ACTIVITY AT CONSTANT AND LINEARLY RISING TEMPERATURES

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