Study of the rat neostriatum using a combined Golgi-electron microscope technique and serial sections

Dimova, R; Vuillet, J.; Seite, R

doi:10.1016/0306-4522(80)90022-6

Cited by 170 publications

(37 citation statements)

References 13 publications

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“…Using radioligand binding morphochemistry, we have previously confirmed the presence of D1 binding sites on cyclic AMP-immunoreactive medium-sized striatal neurons (8)(9)(10). The present data extend these findings in that the fluorescent cellular elements have the cytoarchitectural characteristics of the striatonigral population of the caudate nucleus (22) and are macroscopically organized into a patchy mosaic.…”

Section: Resultssupporting

confidence: 78%

“…These reactive cells are distinctively neuronal in their morphology, having a thin rim of cytoplasm and a soma diameter of =15 ,m, characteristics of the medium spiny neuron in the striatum (22). The largediameter, cholinergic interneuron population in the striatum has also been purported to express D2 receptor binding sites (13), and rhodamine-NAPS was found to be associated with the occasional large neuron in the striatum (Fig.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Direct visualization and cellular localization of D1 and D2 dopamine receptors in rat forebrain by use of fluorescent ligands.

Ariano

Monsma

Barton

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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The regional and cellular localization of the two subtypes of dopamine receptors, DI and D2, have been ascertained in rat forebrain by use of fluorescent dopaminergic antagonist ligands. (R,S)-5-(4'-aminophenyl)-8-chloro-2,3,4,5-tetrahydro-3-methyl-[1lH-3-benzazepin-7-ol, the 4'-amino derivative of the high-affinity Dl-specific antagonist SCH 23390, and the D2 selective antagonist N-(p-aminophenethyl)spiperone were chemically derivatized using the fluorescent compound tetramethylrhodamine. Two distinct populations of dopamine receptors exist in the nervous system (1). The D1 dopamine receptor subtype is positively linked to the activation of the cyclic 3',5'-adenosine monophosphate (cyclic AMP) second messenger system, while the D2 dopamine receptor subtype is linked to a variety of signal transduction mechanisms (2, 3). The pharmacological profiles of these two dopaminergic receptor populations can be discriminated on the basis of their agonist/antagonist specificities (4). The regional distributions of these receptor subtypes have been anatomically distinguished through in vitro autoradiographic localization of radiolabeled antagonist ligands. These previous studies indicate that both receptor subtypes are predominantly located in the basal ganglia and mesolimbic systems (5-7). Cellular localization of the striatal D1 dopamine receptor subtype can be approximated when radioligand binding is combined with the localization of the second messenger, cyclic AMP (8,9). Previous investigations have shown that the cyclic AMPreactive neurons in the caudate nucleus partially project to the substantia nigra (10), and biochemical investigations substantiate that the striatonigral neurons contain a dopamine-sensitive adenylate cyclase activity (11, 12) consistent with the presence of D1 dopamine receptor binding sites. Although the cellular localization of the striatal D2 receptor is unknown, it has been suggested that the cholinergic interneurons of the caudate nucleus have a D2 binding site (13). The recent report ofthe cDNA sequence for the rat brain D2 receptor may help to resolve this issue (14). Another approach taken by some of us (15) has been the specific derivatization of antagonists for the two dopamine receptor classes by using fluorescent moieties. The advantage of application of these derivatized D1-and D2-selective antagonist probes for anatomical determination of the dopamine receptor subtypes is that cellular localization of the binding sites can be distinguished with much higher resolution than by autoradiographic exposure techniques at these magnifications. We report here the regional and cellular localization of the D1 and D2 receptors in fresh-frozen tissue sections of intact rat forebrain using rhodamine-derivatized (R, S)-5-(4'-aminophenyl)-8-chloro-2,3 ,4,5-tetrahydro-3-methyl-[1Hl-3-benzazepin-7-ol (the 4'-amino derivative of SCH 23390) and N-(p-aminophenethyl)spiperone (NAPS) for the D1 and D2 receptors, respectively. METHODSMale Sprague-Dawley rats (200-250 g) were used to provide experim...

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Direct visualization and cellular localization of D1 and D2 dopamine receptors in rat forebrain by use of fluorescent ligands.

Ariano

Monsma

Barton

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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“…Today, the dendrites of these cells are sometimes described as varicose and sometimes as smooth, but the distinguishing feature is still the highly ramified axonal arborization that forms a finely beaded ''web'' of sorts in the region of the dendritic arbor (Jones, 1984). Neurogliaform cells have been reported in numerous brain regions (Jones, 1975;Kemper et al, 1978;Dimova et al, 1980;Chang et al, 1982;McDonald, 1983;Ferrer et al, 1986;Hedlich and Werner, 1986;Kawaguchi, 1995). The dendritic arbor of the cell shown in Figure 14B fits well with Ramón y Cajal's description.…”

Section: Comparison Of Morphological Cell Typessupporting

confidence: 79%

Morphology and physiology of neurons in the rat perirhinal-lateral amygdala area

Faulkner

Brown

1999

J. Comp. Neurol.

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“…The vast majority of the dendrites reconstructed in the present study were traced through several sections, but not up to the cut ends of the slices. The major diameters of dendritic fields of medium-sized spiny neurons in the striatum were reported to be 220-280 µm in the Golgi impregnation studies (Somogyi and Smith, 1979;Dinova et al, 1980;Chang et al, 1982) or 310-450 µm in the in vivo intracellular staining studies (Preston et al, 1980;Wilson and Groves, 1980;Bishop et al, 1982). Because the major diameters of the medium-sized spiny neurons were 330-530 µm in the present study, the reconstructions of dendritic fields of the striatonigral neurons were thought to be nearly complete.…”

Section: Technical Considerationsmentioning

confidence: 74%

“…Because the major diameters of the medium-sized spiny neurons were 330-530 µm in the present study, the reconstructions of dendritic fields of the striatonigral neurons were thought to be nearly complete. On the other hand, the reconstructions of dendrites of the large aspiny cholinergic neurons were considered to be incomplete, because the diameters of dendrites (370-500 µm) were smaller than those reported (410-600 µm; Dinova et al, 1980;Chang et al, 1982;Bishop et al, 1982;Kawaguchi, 1992Kawaguchi, , 1993. Because the fields of axonal arborization were larger than the dendritic fields, axon collaterals of neurons were not recovered completely.…”

Section: Technical Considerationsmentioning

confidence: 79%

Collateral projections from striatonigral neurons to substance P receptor- expressing intrinsic neurons in the striatum of the rat

et al. 1997

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It is well known that striatonigral neurons produce substance P (SP); however, no SP receptor (SPR) has so far been found in the substantia nigra. On the other hand, a previous study in the rat striatum indicated that SPR was expressed only in cholinergic or somatostatinergic intrinsic neurons (Kaneko et al. [1993] Brain Res. 631:297-303). Thus, it was assumed that SP produced by striatonigral neurons might be released through their intrastriatal axon collaterals to act upon intrinsic neurons in the striatum. To confirm this assumption, the distribution of axon collaterals of striatonigral neurons was examined in the striatum of the rat. The experiments were performed on brain slices by combining retrograde labeling with tetramethylrhodamine-dextran amine, electrophysiological recording, intracellular staining with biocytin, and immunocytochemistry for SPR. The distribution of axons of cholinergic striatal neurons (a group of SP-negative intrinsic striatal neurons) was also examined. It was observed that 16% of varicosities of intrastriatal axon collaterals of striatonigral neurons, as well as 6% of axonal varicosities of cholinergic neurons, were in close apposition to dendrites and cell bodies of SPR-immunoreactive striatal neurons. Since SPR-immunoreactive striatal neurons constituted only 2.7% of the total population of striatal neurons (Kaneko et al. [1993] Brain Res. 631:297-303), it appeared that axonal varicosities of striatonigral neurons were preferentially apposed to SPR-immunoreactive striatal neurons and that the varicosities in close apposition to SPR-immunoreactive neurons were derived more frequently from striatonigral neurons than from cholinergic interneurons. Confocal laser scanning microscopy indicated that axonal varicosities in close apposition to SPR-immunoreactive cells showed synaptophysin immunoreactivity, a marker of synaptic vesicles. In intrastriatal axons of striatonigral neurons, it was further revealed from electron microscopy that axonal varicosities in close apposition to SPR-immunoreactive dendrites, at least a part of them, made synapses of the symmetric type. Striatonigral neurons might release SP preferentially around cholinergic or somatostatinergic intrinsic neurons to regulate them through SP-SPR interactions.

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Study of the rat neostriatum using a combined Golgi-electron microscope technique and serial sections

Cited by 170 publications

References 13 publications

Direct visualization and cellular localization of D1 and D2 dopamine receptors in rat forebrain by use of fluorescent ligands.

Direct visualization and cellular localization of D1 and D2 dopamine receptors in rat forebrain by use of fluorescent ligands.

Morphology and physiology of neurons in the rat perirhinal-lateral amygdala area

Collateral projections from striatonigral neurons to substance P receptor- expressing intrinsic neurons in the striatum of the rat

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