Study of the possibility of correcting behavioral disorders in rats with a stereotaxic model of Alzheimer's disease

Abstract

Relevance. Alzheimer's disease (AD) is a neurodegenerative disease, the drug therapy of which can only slow the progression of the disease, due to the variety of existing pathogenetic processes. A possible effective approach to the correction of symptoms can be the use of compounds with a complex mechanism of action — phosphorylacetohydrazides, capable of simultaneously acting on different parts of the pathological process, the most effective representative of which is the compound 2-chloroethoxy-para-N-dimethylaminophenyl phosphorylacetohydrazide (CAPAH). Target. To study the possibility of correcting cognitive and behavioral disorders in rats with a stereotaxic model of AD using the CAPAH compound, which affects different parts of the pathological process. Methods. 24 female Wistar rats were used in the work. AD was modeled in rats by stereotaxic bilateral injection of β-amyloid into the hippocampal region in a phosphate buffer solution, then on day 11, CAPACH (10 mg/kg) was administered intraperitoneally for 10 days, after which tests were performed using the ≪Elevated Plus Maze≫, ≪Open Field≫ and ≪Morris Water Maze≫. Statistical processing was carried out in the GraphPad Prism 8.0.1 program using one-way ANOVA analysis. Results. Multiple administration of CAPAH contributed to a decrease in the level of anxiety in the ≪Elevated Plus Maze≫ method, increasing the time spent in open arms by 4.6 times (p < 0.05) compared to rats without treatment. In the ≪Morris Water Maze≫ and ≪Open Field≫ tests, normalization of memory and motor activity processes was observed, respectively, the platform search time and the number of crossed lines did not differ from those of control animals. Conclusion. CAPAH reduces anxiety and memory processes in rats with a stereotaxic model of Alzheimer's disease caused by the introduction of β-amyloid into the hippocampus.