Study of the possibility of increasing the probing depth by the method of reflection confocal microscopy upon immersion clearing of near-surface human skin layers

Meglinskii, I V; Bashkatov, Alexey N.; Genina, Elina A.; Churmakov, Dmitry Y.; Тучин, В. В.

doi:10.1070/qe2002v032n10abeh002309

Cited by 52 publications

(29 citation statements)

References 14 publications

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“…In both our experience and reports by other groups, RCM images have been acquired at >300 μm in the oral epithelium [42]. While this penetration depth is relatively low in comparison to other imaging modalities such as optical coherence tomography and multi-photon microscopy, most normal values of epithelial thickness within the oral cavity have been noted to be less than 300 μm [39].…”

Section: Discussionmentioning

confidence: 73%

A novel multimodal optical imaging system for early detection of oral cancer

Malik

Jabbour

Cheng

et al. 2016

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Objectives Several imaging techniques have been advocated as clinical adjuncts to improve identification of suspicious oral lesions. However, these have not yet shown superior sensitivity or specificity over conventional oral examination techniques. We developed a multimodal, multi-scale optical imaging system that combines macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic imaging of reflectance confocal microscopy (RCM) for early detection of oral cancer. We tested our system on excised human oral tissues. Study Design A total of four tissue specimen were imaged. These specimens were diagnosed as one each: clinically normal, oral lichen planus, gingival hyperplasia, and superficially-invasive squamous cell carcinoma (SCC). The optical and fluorescence lifetime properties of each specimen were recorded. Results Both quantitative and qualitative differences between normal, benign and SCC lesions can be resolved with FLIM-RCM imaging. The results demonstrate that an integrated approach based on these two methods can potentially enable rapid screening and evaluation of large areas of oral epithelial tissue. Conclusions Early results from ongoing studies of imaging human oral cavity illustrate the synergistic combination of the two modalities. An adjunct device based on such optical characterization of oral mucosa can potentially be used to detect oral carcinogenesis in early stages.

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Section: Discussionmentioning

confidence: 73%

A novel multimodal optical imaging system for early detection of oral cancer

Malik

Jabbour

Cheng

et al. 2016

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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“…52. The stochastic numerical MC method is widely used to model optical radiation propagation in complex randomly inhomogeneous highly scattering and absorbing media such as biological tissues 9,26,35,36 Table 1. …”

Section: Methodsmentioning

confidence: 99%

<title>Monte Carlo study of skin optical clearing to enhance light penetration in the tissue</title>

Bashkatov¹,

Genina²,

Tuchin³

et al. 2007

SPIE Proceedings

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Result of Monte Carlo simulations of skin optical clearing is presented. The model calculations were carried out with the aim of studying of spectral response of skin under immersion liquids action and calculation of enhancement of light penetration depth. In summary, we have shown that: 1) application of glucose, propylene glycol and glycerol produced significant decreasing of light scattering in different skin layers; 2) maximal clearing effect will be obtained in case of optical clearing of skin dermis, however, absorbed light fraction in skin dermis changed insignificantly, independently from clearing agent and place it administration; 4) in contrast to it, the light absorbed fraction in skin adipose layer increased significantly in case of optical clearing of skin dermis. It is very important because it can be used for development of optical methods of obesity treatment.

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“…Current approach has applied for a theoretical examination of how localized skin-tissue dehydration affects the depth of the confocal probing and what depth of effective detection can be reached with the chemical administration of skin tissues [27][28] . A solution of glycerol was taken in the capacity of innocuous osmotic agent.…”

Section: Various Applications Of the Technique In Optical Diagnosticsmentioning

confidence: 99%

A novel Monte Carlo method for the optical diagnostics of skin

Meglinski

Churmakov

2003

SPIE Proceedings

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A novel Monte Carlo (MC) technique for photon migration through 3D media with the spatially varying optical properties is presented. The employed MC technique combines the statistical weighting variance reduction and real photon paths tracing schemes. The overview of the results of applications of the developed MC technique in optical/near-infrared reflectance spectroscopy, confocal microscopy, fluorescence spectroscopy, OCT, Diffusing Wave Spectroscopy (DWS) and Doppler flowmetry are presented.

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Study of the possibility of increasing the probing depth by the method of reflection confocal microscopy upon immersion clearing of near-surface human skin layers

Cited by 52 publications

References 14 publications

A novel multimodal optical imaging system for early detection of oral cancer

A novel multimodal optical imaging system for early detection of oral cancer

<title>Monte Carlo study of skin optical clearing to enhance light penetration in the tissue</title>

A novel Monte Carlo method for the optical diagnostics of skin

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