Study of the mechanisms underlying increased glucose absorption in<i>Smilax china</i>L. leaf extract-treated HepG2 cells

Kang, Yun Hwan; Kim, Dae Jung; Kim, Kyoung Kon; Lee, Sung Mee; Choe, Myeon

doi:10.4163/jnh.2014.47.3.167

J Nutr Health

2014

DOI: 10.4163/jnh.2014.47.3.167

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Study of the mechanisms underlying increased glucose absorption inSmilax chinaL. leaf extract-treated HepG2 cells

Yun Hwan Kang

Dae Jung Kim

Kyoung Kon Kim

et al.

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Cited by 3 publications

References 25 publications

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Modulation of Glucose Consumption and Uptake in HepG2 Cells by Aqueous Extracts from the Coelomic Fluid of the Edible Holothuria tubulosa Sea Cucumber

Abruscato,

Tarantino,

Mauro

et al. 2024

Biology

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The cell-free aqueous extract from the coelomic fluid of Holothuria tubulosa was prepared and examined for its glucose-lowering effect on HepG2 cells in vitro. In particular, employing a combination of cytochemical, flow cytometric, PCR, and protein blot techniques, we evaluated its role on glucose internalization and storage and on the upregulation and surface translocation of the two glucose transporters GLUT-2 and -4. The changes in expression, synthesis, and/or activation of the GLUT2-related transcription factor hepatocyte nuclear factor-1 alpha (HNF1α) and the GLUT-4-translocation regulatory factors insulin receptor substrate-1 (IRS-1) and AKT were also studied. Our results showed the improved glucose response by HepG2 cells, leading to an evident increase in glucose consumption/uptake and glycogen storage upon exposure. Moreover, the extract induced molecular reprogramming involving the upregulation of (i) IRS1 gene expression, (ii) the transcription and translation levels of HNF1α, AKT, and GLUT-4, (iii) the phosphorylation level of AKT, (iv) the synthesis of GLUT-2 protein, and (v) the translocation of GLUT-2 and -4 transporters onto the plasma membrane. Cumulatively, our results suggest that the coelomic fluid extract from H. tubulosa can be taken into consideration for the development of novel treatment agents against diabetes mellitus.

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Modulation of Glucose Consumption and Uptake in HepG2 Cells by Aqueous Extracts from the Coelomic Fluid of the Edible Holothuria tubulosa Sea Cucumber

Abruscato,

Tarantino,

Mauro

et al. 2024

Biology

View full text Add to dashboard Cite

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TheIn VitroAntioxidant and Antidiabetic Activity of Various Fractions from Monascus-Fermented Ginger Extract

Yoon

Pyo

2022

JKFN

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This study investigated the potential antioxidant and antidiabetic activity of Monascus-fermented ginger extract (MGE). Gingers was fermented with Monascus pilosus KCCM 60084 for 2 weeks at 30°C and extracted with 95% ethanol. The antioxidant activity of the ethyl acetate fraction of the MGE was measured through DPPH, ABTS, and FRAP. The antioxidant activity of MGE increased 2.36 to 3.76-fold compared with those of raw ginger extracts (CGE) (P<0.05). The ethyl acetate fraction of MGE had a higher potential to inhibit the key digestive enzymes linked to type 2 diabetes (α-amylase, α-glucosidase) as compared to CGE (P<0.05). The IC50 for the α-amylase inhibitory activity (3.40±0.88 mg/mL) of MGE showed a higher potency than acarbose (7.01±0.23 mg/mL), which is currently used as a diabetes treatment. Moreover, the ethyl acetate fraction of MGE increased the expression of glucose transporter type 4 (GLUT4), glucokinase (GCK), and AMP-activated protein kinase (AMPK) in HepG2 cells. These findings demonstrate that the ethyl acetate fraction of MGE significantly improved glucose uptake in HepG2 cells by activating both PI3K/Akt and AMPK phosphorylation pathways. Our results suggest that MGE show the potential to be developed as a antidiabetic nutraceuticals.

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Effect of amaranth seed extracts on glycemic control in HepG2 cells

Park

Hwang

2021

J Nutr Health

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Study of the mechanisms underlying increased glucose absorption inSmilax chinaL. leaf extract-treated HepG2 cells

Cited by 3 publications

References 25 publications

Modulation of Glucose Consumption and Uptake in HepG2 Cells by Aqueous Extracts from the Coelomic Fluid of the Edible Holothuria tubulosa Sea Cucumber

Modulation of Glucose Consumption and Uptake in HepG2 Cells by Aqueous Extracts from the Coelomic Fluid of the Edible Holothuria tubulosa Sea Cucumber

TheIn VitroAntioxidant and Antidiabetic Activity of Various Fractions from Monascus-Fermented Ginger Extract

Effect of amaranth seed extracts on glycemic control in HepG2 cells

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