Study of the Mass Transfer Kinetics of BSA on a TSK-GEL DEAE-5PW Anion Exchanger in a Wide Concentration Range

Guan-Sajonz, Hong; Sajonz, Peter; Zhong, Guoming; Guiochon, Georges

doi:10.1021/bp960025v

Cited by 47 publications

(41 citation statements)

References 17 publications

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“…dependence of both km,L of BSA 17,18 and km of Tröger's base 19 reported previously [8][9][10][11] and derived new information concerning the mass transfer characteristics of the four transport processes described above. In the case of anion exchange separation of BSA, 17,18 it was shown that surface diffusion played a predominant contribution in the kinetic properties of mass transfer and that the linear concentration dependence of km,L probably resulted from that of the surface diffusion coefficient (Ds).…”

supporting

confidence: 61%

“…The extent of the positive concentration dependence of km,L was larger for L-PA than for D-PA. It is expected that a more detailed analysis of the overall mass transfer rate parameter, km,L, could provide new, useful information and allow for a better understanding of the kinetic properties of imprinted stationary phases.A similar positive concentration dependence was also demonstrated for km,L in anion exchange chromatography of bovine serum albumin (BSA), 8,9 and for the mass transfer rate coefficient (km) in the enantiomeric separation of Tröger's base on microcrystalline cellulose triacetate. 10,11 In other previous publications, a concentration dependence was also confirmed for intraparticle diffusivity, 12 for tracer diffusivity, 13 for axial dispersion, 14 for effective diffusivity, 15 and for mutual diffusion coefficients.…”

supporting

confidence: 56%

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Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Miyabe

Guiochon

2000

ANAL. SCI.

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Molecular imprinting is a most attractive method of preparation of dedicated stationary phases for chromatography. Its use for enantioseparations is attracting considerable interest. 1,2 Imprinted materials can provide a very high selectivity for the objective compound, i.e., the imprinted molecule. [1][2][3][4][5] Although analytical applications are rapidly developing, the situation on the preparative front is less rosy. 1 Columns packed with imprinted stationary phases exhibit a poor efficiency and elution peaks are often unsymmetrical, especially for the more retained enantiomer. Sellergren et al. 1,2 suggested that the nonlinear behavior of the adsorption isotherm was the main cause of the broad and unsymmetrical bands observed for L-phenylalanine anilide (PA) on an L-PA imprinted chiral stationary phase. Although the possibility of a slow mass transfer kinetics was also pointed out, no detailed study supported this assumption. Yet, information on the mass transfer characteristics of imprinted stationary phases is necessary to clarify strategies for the improvement of these materials.Most kinetic studies in chromatography assume that four mass transfer processes are involved in a column, (1) axial dispersion, (2) fluid-to-particle mass transfer, (3) intraparticle diffusion, and (4) adsorption/desorption. 6 The contributions of these four processes to peak spreading are evaluated separately. Axial dispersion and the fluid-to-particle mass transfer resistance are inherent to flow processes through packed beds. They are well understood, relatively easy to control, and their contributions are often small. By contrast, intraparticle diffusion and adsorption/desorption give often larger contributions to the mass transfer resistance in the column. Their properties are intrinsic to each packing material and they are still often poorly understood. In order to evaluate the actual performance of packing materials, the characteristics of the mass transfer kinetics inside stationary phase particles must be clarified.Sajonz et al. 7 measured the adsorption equilibrium isotherm and the mass transfer kinetics of L-and D-PA on a polymeric stationary phase imprinted with L-PA, using a staircase frontal analysis method. The isotherm was well represented by either the Bi-Langmuir or the Freundlich models, but not by the simple Langmuir isotherm. The equilibrium data suggested that the distribution of the adsorption energy on the surface of the imprinted stationary phase is heterogeneous and showed that this phase has a high selectivity for the imprinted L-enantiomer. The lumped mass transfer rate coefficient (km,L) was calculated from each single breakthrough curve by applying the transport model of chromatography. 6 The values of km,L increased with increasing concentration of the enantiomers. The extent of the positive concentration dependence of km,L was larger for L-PA than for D-PA. It is expected that a more detailed analysis of the overall mass transfer rate parameter, km,L, could provide new, useful information and all...

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confidence: 61%

supporting

confidence: 56%

Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Miyabe

Guiochon

2000

ANAL. SCI.

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“…However, the discrepancy between it and the theoretical curve calculated by the E-D model turns out to be significant. This result is as expected, because the E-D model will not be suited for affinity separations where the kinetics are slow [28][29][30][31]. The Thomas model concerns the effect of sorption kinetics and has been used to describe the breakthrough curves obtained in MAC [12,18].…”

Section: Application Of the Mp Model In Macsupporting

confidence: 63%

Evaluation of nonlinear chromatographic performance by frontal analysis using a simple multi-plate mathematical model

Wang

2005

Journal of Chromatography A

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“…It is assumed that k m,L is constant during each concentration step in the FA measurement and that k m,L corresponds to the average concentration of this step. By using this method, Guan-Sajonz et al [27] and Sajonz et al [28] measured the rate of the mass transfer kinetics of bovine serum albumin in ion exchange chromatography, using anion exchange resins. Seidel-Morgenstern et al [29] and Rearden et al [30] studied the mass transfer kinetics of Tröger's base in the phase system of cellulose triacetate and ethanol.…”

Section: Fluorescence Relaxationmentioning

confidence: 99%

Measurement of the parameters of the mass transfer kinetics in high performance liquid chromatography

Miyabe

Guiochon²

2003

J of Separation Science

148

161

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Fundamental studies of the mass transfer kinetics are as essential as those of the retention equilibrium for a detailed understanding of the characteristics and the mechanisms of chromatographic separations. The acquisition of a large amount of reliable experimental data and of meaningful results is necessary for any further progress of our knowledge of kinetics. The main goal of this review is to provide information on the methods used to perform accurate measurements and on the data analysis procedures used for deriving the kinetic parameters characterizing mass transfer in HPLC. First, the general characteristics of several methods of determination of some kinetic parameters are briefly reviewed. Secondly, we give detailed explanations of the experimental conditions of the pulse on a plateau method (i.e., elution chromatography on a plateau of finite concentration or pulse response method) and of the data analysis procedures based on moment analysis. Thirdly, we explain some important requirements for the acquisition of appropriate experimental data and discuss corrections to be applied when deriving several kinetic parameters. Fourthly, we discuss the accuracy of the kinetic parameters derived from the pulse on a plateau method and from moment analysis. Finally, some results concerning the mass transfer kinetics in RPLC systems are demonstrated as examples.

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Study of the Mass Transfer Kinetics of BSA on a TSK-GEL DEAE-5PW Anion Exchanger in a Wide Concentration Range

Cited by 47 publications

References 17 publications

Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Evaluation of nonlinear chromatographic performance by frontal analysis using a simple multi-plate mathematical model

Measurement of the parameters of the mass transfer kinetics in high performance liquid chromatography

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