Study of the Luteinizing Hormone-Induced Increase of Ovarian Blood Flow During the Estrous Cycle in the Rat

Varga, B; Horvath, Edit; Folly, G; Stark, E.

doi:10.1095/biolreprod32.3.480

Cited by 32 publications

(13 citation statements)

References 24 publications

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“…In the previous studies in cattle (Acosta et al 2003), rats (Varga et al 1985), rabbits (Janson 1975) and sheep (Niswender et al 1976), the role of oestradiol and LH on blood flow of the follicle wall was not well established. An increase of blood-flow velocity (TAMV) in the vessel of the follicular wall occurred 0.5 h after GnRH treatment, synchronously with an increase in systemic concentrations of oestradiol and LH (Acosta et al 2003); follicular blood flow remained high, while systemic concentrations of oestradiol and LH returned to basal level by 6 h and remained low until ovulation.…”

Section: Discussionmentioning

confidence: 97%

Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers

Siddiqui¹,

Gastal²,

Gastal³

et al. 2009

Reproduction

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The effect of the extent of vascular perfusion of the wall of the preovulatory follicle on in vitro cleavage rate of the recovered oocyte and embryo development to >8 cells was studied in 52 heifers. Heifers received a luteolytic dose of prostaglandin F2α (PGF2α) when the largest follicle was ≥11 mm. An ovulation-inducing injection of GnRH was given 36 h later (hour 0), and collection of follicular fluid and the oocyte was done at hour 26. Vascular perfusion of the follicular wall was assessed by colour Doppler ultrasonography at hours 0 and 26. Each of the recovered oocytes (41/52; 79%) was mature (extruded polar body). Cleavage and embryo development were assessed at 48 h and 120 h respectively, after in vitro fertilisation (IVF). The percentage of cleaved oocytes and >8 cell embryos was 80% (31/39) and 55% (17/31) respectively. Vascular perfusion of the follicular wall was greater (lower pulsatility index; P<0.001) for follicles that produced cleaved versus non-cleaved oocytes and greater (P<0.04) for follicles that produced >8 cell versus ≤8 cell embryos. Percentage of follicular wall with Doppler signals of blood flow was greater (P<0.001) for >8 cell versus ≤8 cell embryos. Follicular-fluid concentration of free IGF1 was lower for cleaved oocytes (P<0.001) and >8 cell embryos (P<0.05), and oestradiol was lower (P<0.05) for >8 cell embryos. Results supported the hypothesis that greater vascular perfusion of the wall of the preovulatory follicle was positively associated with IVF and embryo development.

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Section: Discussionmentioning

confidence: 97%

Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers

Siddiqui¹,

Gastal²,

Gastal³

et al. 2009

Reproduction

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“…In addition, a beta adrenergic mechanism is involved in steroid-induced increases in ovarian blood flow (Varga et al, 1985) as well as in the facilitation of LH-induced steroidogenesis in hypertrophied thecal and secondary interstitial cells (Erickson et al, 1985). Moreover, these cells have been shown to receive direct adrenergic innervation (Erickson et al, 1985).…”

mentioning

confidence: 99%

Development and maintenance of a polycystic condition in ovaries autotransplanted under the kidney capsule

Desjardins

Brawer

1989

Anat. Rec.

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A single injection of estradiol valerate (EV) produces a polycystic ovarian (PCO) condition in the rat. The development of the PCO condition coincides with alterations in the endogenous plasma gonadotropin patterns, suggesting that PCO may be a response to abnormal gonadotropin stimulation. Other factors, however, such as direct autonomic innervation, also contribute significantly to the regulation of the ovary and could be important in generating and/or maintaining PCO. We have, therefore, removed and autotransplanted one ovary in each of eight rats under the capsule of the ipsilateral kidney, thus totally disrupting its innervation. The animals were injected with EV and both ovaries of each animal were examined 8 weeks later. In a second group of animals, we induced the PCO condition, autotransplanted one polycystic ovary in each animal under the kidney capsule, and examined the ovaries 2 weeks later. In both groups the autotransplanted ovaries exhibited the full range of polycystic morphology, as did the intact ovary in each animal. We conclude that since a major perturbation in innervation affects neither the development nor the maintenance of PCO, autonomic innervation does not play a crucial role in this disorder.

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“…The estrogen concentration appears to be only one of many factors that affect follicular blood flow; our results showed that follicular blood flow continued to increase from Day -1 to Day 0 in the 1 st wave cycles even though the concentration of estrogen decreased during the same time period. For example, it has been demonstrated that LH induces an increase in follicular blood flow in rats by decreasing vascular resistance in the rat ovary [23]. Furthermore, the results of several studies indicate that there is crosstalk between vascular components (endothelial cells) and follicular steroidogenic cells [24,25] that may modulate the local changes of blood flow in ovulatory follicles [26].…”

Section: Discussionmentioning

confidence: 99%

Genital Blood Flow and Endometrial Gene Expression During the Preovulatory Period after Prostaglandin F2.ALPHA.-Induced Luteolysis in Different Luteal Phases in Cows

Jordan

Herzog

Ulbrich

et al. 2009

Journal of Reproduction and Development

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Abstract. The aim of this study was to examine the hypothesis that follicular and uterine perfusion as well as endometrial gene expression during the ovulatory period differs after induction of luteolysis during the 1 st follicular wave compared with the 2 nd wave or in intact cycle. Nine healthy non-lactating Holstein-Friesian cows were examined during three estrous cycles. A prostaglandin F2α analogue (PGF) was administered randomly either on Day 7 (1 st wave cycle) or Day 11 (2 nd wave cycle) after detection of ovulation (=Day 1). No hormonal treatment was used in the intact cycle with spontaneous ovulation. Transrectal Doppler sonography was conducted daily after PGF injection and in the intact cycle beginning on Day 18 of the estrous cycle until ovulation. Follicular blood flow (FBF) was determined by measuring the maximum area of colour pixels on digitalized images of the follicles. Uterine blood flow was quantified by the time-averaged maximum velocity (UTAMV) and pulsatility index (PI) in both uterine arteries. Blood flow measurements were carried out on Days -1 and 0. Endometrial biopsy specimens were taken on Day 1 and analyzed for the gene expressions of estrogen, progesterone, oxytocin and VEGF receptors and eNOS and iNOS using RT-PCR. The interval from PGF injection to ovulation was shorter (P<0. [309][310][311][312][313][314][315] 2009) here are several reports about differences in the size and contents of follicles of the first and other follicular waves in cows [1][2][3][4]. Follicular fluid of the dominant follicle of the first follicular wave shows a higher concentration of estradiol than fluid of follicles of the second wave [2]. Furthermore, thecal cells from the dominant follicle of the first wave cultured in vitro secrete more progesterone than those of the dominant follicle of the second wave. Despite lower estradiol and progesterone concentrations, dominant follicles of the second wave are larger than those of the first wave [2]. However, the size of the dominant follicle is not an indicator of its functional status [3,4]. In cycling cows, angiogenesis, as determined by colour Doppler sonography, is a good parameter for evaluation of the functional status of the follicle. Follicular perfusion is markedly higher in dominant follicles than in subdominant follicles [5], and the walls of atretic follicles lack blood flow [6]. In a recent colour Doppler sonographic study, the vascularity of the preovulatory follicle was investigated in heifers before insemination. The results supported the hypothesis of a positive relationship between the extent of blood flow of the preovulatory follicle and successful establishment of pregnancy in cattle [7].The technique of colour Doppler sonography allows not only the determination of ovarian perfusion, but also measurement of uterine blood supply [8]. Although up to now only little information has been available in cows about changes in uterine blood flow during the estrous cycle [8] and pregnancy [9,10], a relationship between uterine perfusion and fe...

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Study of the Luteinizing Hormone-Induced Increase of Ovarian Blood Flow During the Estrous Cycle in the Rat

Cited by 32 publications

References 24 publications

Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers

Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers

Development and maintenance of a polycystic condition in ovaries autotransplanted under the kidney capsule

Genital Blood Flow and Endometrial Gene Expression During the Preovulatory Period after Prostaglandin F2.ALPHA.-Induced Luteolysis in Different Luteal Phases in Cows

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