Study of the iodide binding protein of the plasma of the rainbow trout (Salmo gairdnerii Richardson)

Perkier, Henri; Perkier, C.; Delcroix, J.P.; Bornet, H; Gras, J

doi:10.1016/0300-9629(76)90086-4

Comparative Biochemistry and Physiology Part A: Physiology

1976

DOI: 10.1016/0300-9629(76)90086-4

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Study of the iodide binding protein of the plasma of the rainbow trout (Salmo gairdnerii Richardson)

Henri Perkier¹,

C. Perkier²,

J.P. Delcroix

et al.

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1978

1991

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Cited by 3 publications

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A novel method for specific visualisation of serum albumin in polyacrylamide gels by iodine staining

Lee

Ellis

1991

Electrophoresis

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Bovine serum, bovine serum albumins (delipidated or globulin free or Fraction V), rabbit serum, rabbit serum albumin, Atlantic salmon serum, purified Atlantic salmon serum albumin, human plasma alpha 2-macroglobulin, hemocyanin, trypsin inhibitor, bovine transferrin and bovine lactoferrin were examined by a novel method for specific visualisation of albumins. In native polyacrylamide gel electrophoresis the albumins were visualised by iodine staining as a transparent spot against a brown background whilst the other proteins could not be visualised. It is suggested that the brown background was due to penetration of the gel by iodine while the chemical binding of iodine by albumin produced a decolourisation reaction. This novel method provides a fast and simple approach to identifying serum albumin in polyacrylamide gels.

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A novel method for specific visualisation of serum albumin in polyacrylamide gels by iodine staining

Lee

Ellis

1991

Electrophoresis

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Studies on the rainbow trout (Salmo gairdneri Rich.). I. Correlation between gonadal development and serum protein pattern

Borchard¹

1978

Ann. Biol. anim. Bioch. Biophys.

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Summary. Maturity was studied on 273 rainbow trout of both sexes. The female gonads were classed into 3 maturation stages and the male gonads into 4. Characteristic pherograms of both serum protein electrophoresis and total serum protein corresponded with maturation stage. Electrophoretic globulin and total protein fractions increased considerably with spermatogenetically active testes and mature ovaries ; the A/G ration decreased accordingly. These parameters can be used to determine maturity.Introduction.

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A literature review of the blood chemistry of rainbow trout, Salmo gairdneri Rich.

Hille

1982

Journal of Fish Biology

147

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Study of the iodide binding protein of the plasma of the rainbow trout (Salmo gairdnerii Richardson)

Cited by 3 publications

References 3 publications

A novel method for specific visualisation of serum albumin in polyacrylamide gels by iodine staining

A novel method for specific visualisation of serum albumin in polyacrylamide gels by iodine staining

Studies on the rainbow trout (Salmo gairdneri Rich.). I. Correlation between gonadal development and serum protein pattern

A literature review of the blood chemistry of rainbow trout, Salmo gairdneri Rich.

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