Study of the Influence of Pulse Infrared Radiation on Optical and Hemolysis Resistant Properties of Erythrocytes

Abstract

Resistance to hemolysis of erythrocytes in lysing isotonic water solution of ammonium chloride becomes apparent in the dynamics of the change of their optical properties. Optical properties of erythrocytes were registered on light scattering from single cells with a scanning flow cytometer. It was shown experimentally during hemolysis the dynamics of the change of the integral intensity of light scattering of erythrocytes is different for irradiated and non-irradiated human blood. Qualitatively, the effect is similar for the both sources of pulse radiation: at the beginning of hemolysis the mean integral intensity of irradiated erythrocytes is increasing more than that is of non-irradiated ones. Quantitatively, at the same mean power density the interrupted irradiation of CO2 laser caused greater effect than continuous one, but less than pulsed terahertz irradiation, i.e. the effect is greater for shorter pulse. On the base of the obtained results one can suggest that the observed effect on pulse infrared radiation of low mean power caused by unsteady heating of erythrocytes.