Study of the in vitro degradation and characterization of the HaCat keratinocytes adherence on electrospun scaffolds based polyvinyl alcohol/sodium alginate

Abstract

Biomaterial scaffolds house and direct cells to grow, exposing them to appropriate growth factors. Most of them are designed to degrade at a controlled rate as the new host tissue replaces them. Here, we show that scaffolds obtained through electrospinning of PVA with 3.5, 4.0, and 5.0 wt% of SA showed a gradual degradation rate under physiological conditions during 100 days of incubation. This behavior is proportional to the SA concentration used, with a mass loss of (41%, 47%, and 49% for each system) after 60 days. Moreover, the dielectric properties (EIS) of the scaffolds correlate with their degradation rate throughout the exposition period (100 days), as indicated by the observed decrease in resistance over time. The 4.0 wt% system showed the lowest capacitance value (1.5 10–5 F) and the highest resistance (4.6106 ohm‐cm2) behavior among all concentrations. Additionally, the morphological analysis through SEM and AFM showed a shrank in the surface morphology after the degradation process and determined the degree of roughness (Ra). Higher roughness prior to the cytocompatibility test promoted increased proliferation and adhesion of HaCat cells, as observed in the 4.0 wt% PVA/SA system (1422 nm Ra). These biomaterials have great potential in wound dressing applications.

Development and optimisation of hydroxyapatite-polyethylene glycol diacrylate hydrogel inks for 3D printing of bone tissue engineered scaffolds

“…For scaffolds with 2 wt% HA, the degradation rate appeared to reach a plateau from day 7, while scaffolds with 5 wt% HA showed a slowing down or reaching a steady state of degradation by day 14 after an initial period of mass loss. These time points correspond to the equilibrium point of degradation rate, where minimal or no changes in mass loss occur until the end of the assay [60]. This behaviour may be attributed to the release of HA into the media, where the higher HA content and more compact structure of scaffolds with 5 wt% HA prolonged the time needed to reach the equilibrium point compared to scaffolds with 2 wt% HA.…”

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

“…The degree of roughness of the electrospun scaffolds was determined using SEM and AFM, and it was discovered that higher roughness allowed for increased proliferation and adhesion of HaCaT cells, indicating that microgeometry and surface roughness of biomaterials can affect cell behavior. 51 Bacterial nanocellulose derived from different strains of bacteria was studied as a potential wound dressing. Results showed that the density of the nanocellulose could be controlled based on the strain from which it was derived, and that wound repair efficacy could be improved by treatment with lower density of nanocellulose.…”

“…Another study investigated the effect of alginate composition on electrospinning of PVA and its eventual degradation rate in vitro. The degree of roughness of the electrospun scaffolds was determined using SEM and AFM, and it was discovered that higher roughness allowed for increased proliferation and adhesion of HaCaT cells, indicating that microgeometry and surface roughness of biomaterials can affect cell behavior 51 . Bacterial nanocellulose derived from different strains of bacteria was studied as a potential wound dressing.…”