Study of the immunomodulatory effects of osteogenic differentiated human dental pulp stem cells

Hossein‐Khannazer, Nikoo; Hashemi, Seyed Mahmoud; Namaki, Saeed; Ghanbarian, Hossein; Sattari, Mandana; Khojasteh, Arash

doi:10.1016/j.lfs.2018.11.040

Cited by 44 publications

(36 citation statements)

References 9 publications

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“…In particular, TLR and NO production may participate in the antimicrobial pulp response in an iNOS-dependent or -independent manner. 19,21,100,[105][106][107] iNOS synthesis and subsequent NO production can be served as part of the immunologic defense in human physiology and participate in the elimination of invading pathogens, including oral pathogens (e.g., cariogenic Streptococcus mutans). 79,108 Previous studies have indicated a concentration-dependent antibacterial effect of NO on endodontic microorganisms and its primary role in the clearance of Enterococcus faecalis by macrophages.…”

Section: Anmentioning

confidence: 99%

“…7,18 Preexisting and/or newly formed odontoblasts from DPCs within the pulp are believed to be responsible for the innate immunity and mineralization of the reparative dentin matrix. [19][20][21] Thus, this review article summarizes the physiological and pathological effects of the NO system on DPCs/odontoblasts and other cell lineages involved in immune responses inside dental pulp tissue and then discuss its potential role as a therapeutic target and tool to deal with dental pulp inflammation.…”

Section: Introductionmentioning

confidence: 99%

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Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

2020

Tissue Engineering Part B: Reviews

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Nitric oxide (NO), which is synthesized by the enzyme NO synthase (NOS), is a versatile endogenous molecule with multiple biological effects on many tissues and organs. In dental pulp tissue, NO has been found to play multifaceted roles in regulating physiological activities, inflammation processes, and tissue repair events, such as cell proliferation, neuronal degeneration, angiogenesis, and odontoblastic differentiation. However, there is a deficiency of detailed discussion on the NO-mediated interactions between inflammation and reparative/ regenerative responses in wounded dental pulp tissue, which is a central determinant of ultimate clinical outcomes. Thus, the purpose of this review is to outline the current molecular understanding on the roles of Janusfaced molecule NO in dental pulp physiology, inflammation, and reparative activities. Based on this knowledge, advanced physicochemical techniques designed to manipulate the therapeutic potential of NOS and NO production in endodontic regeneration procedures are further discussed.

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Section: Anmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

2020

Tissue Engineering Part B: Reviews

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“…In addition to their osteo/odontogenic differentiation capabilities, hDPSCs exhibit highly desirable immunomodulatory properties, making them a promising therapeutic cell source for clinical use (Andrukhov et al, 2019;Hossein-Khannazer et al, 2019). Multiple studies have reported that no clinical or histological rejection of hDPSCs when implanted in animals without immunosuppression (de Mendonca Costa et al, 2008;Fernandes et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Use of Human Dental Pulp and Endothelial Cell Seeded Tyrosine-Derived Polycarbonate Scaffolds for Robust in vivo Alveolar Jaw Bone Regeneration

Zhang

Saxena

Fakhrzadeh

et al. 2020

Front. Bioeng. Biotechnol.

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The ability to effectively repair craniomaxillofacial (CMF) bone defects in a fully functional and aesthetically pleasing manner is essential to maintain physical and psychological health. Current challenges for CMF repair therapies include the facts that craniofacial bones exhibit highly distinct properties as compared to axial and appendicular bones, including their unique sizes, shapes and contours, and mechanical properties that enable the ability to support teeth and withstand the strong forces of mastication. The study described here examined the ability for tyrosine-derived polycarbonate, E1001(1K)/β-TCP scaffolds seeded with human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) to repair critical sized alveolar bone defects in an in vivo rabbit mandible defect model. Human dental pulp stem cells are uniquely suited for use in CMF repair in that they are derived from the neural crest, which naturally contributes to CMF development. E1001(1k)/β-TCP scaffolds provide tunable mechanical and biodegradation properties, and are highly porous, consisting of interconnected macro-and micropores, to promote cell infiltration and attachment throughout the construct. Human dental pulp stem cells/HUVECs seeded and acellular E1001(1k)/β-TCP constructs were implanted for one and three months, harvested and analyzed by micro-computed tomography, then demineralized, processed and sectioned for histological and immunohistochemical analyses. Our results showed that hDPSC seeded E1001(1k)/β-TCP constructs to support the formation of osteodentinlike mineralized jawbone tissue closely resembling that of natural rabbit jaw bone. Although unseeded scaffolds supported limited alveolar bone regeneration, more robust and homogeneous bone formation was observed in hDPSC/HUVEC-seeded constructs, suggesting that hDPSCs/HUVECs contributed to enhanced bone formation. Importantly, bioengineered jaw bone recapitulated the characteristic morphology of natural rabbit jaw bone, was highly vascularized, and exhibited active remodeling by the presence of osteoblasts and osteoclasts on newly formed bone surfaces. In conclusion, these results demonstrate, for the first time, that E1001(1K)/ β-TCP

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“…In our previous study, we showed that DPSCs could inhibit the proliferation of PBMCs after 72 hours [15]. Tang et al also reported that DPSCs had inhibitory effects on the proliferation of PBMCs [16].…”

Section: Discussionmentioning

confidence: 86%

The Effects of Dental Pulp Stem Cell Conditioned Media on the Proliferation of Peripheral Blood Mononuclear Cells

et al. 2019

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Background: Dental Pulp Stem Cells (DPSCs) are multipotent mesenchymal stem cells. DPSCs can renew themselves and differentiate into various cell types such as adipocytes, osteocytes, neurons, etc. DPSCs possess immunomodulatory properties and can inhibit Peripheral Blood Mononuclear Cell (PBMC) proliferation. Recent studies showed that conditioned-medium mesenchymal stem cells also had immunosuppressive activity. The ability of DPSC conditioned medium to suppress proliferation of allogeneic PBMC determined using BrdU (5-bromo-2'-deoxyuridine) proliferation assay. Materials and Methods:Dental pulp stem cells were extracted from a wisdom tooth. These cells are characterized for differentiation potential to adipogenic and osteogenic lineage and expression of mesenchymal stem cells markers, including CD105, CD73, CD90, CD14, CD-34, and CD45. The characterized DPSCs were cultured, and the Conditioned Medium (CM) got isolated. Stimulated and non-stimulated PBMCs from the allogeneic donor were cultured with DPSC-CM for 24, 48, and 72 hours. The proliferation of PBMCs was measured with BrdU assay. Results:The BrdU test results showed that DPSC-CM reduced allogeneic PBMC proliferation at different time points. DPSC-CM could inhibit stimulated and non-stimulated PBMC in 48 and 72 hours after incubation. Conclusion:This study demonstrated that DPSC-CM had an immunomodulatory effect on the proliferation of allogeneic cells.

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Study of the immunomodulatory effects of osteogenic differentiated human dental pulp stem cells

Cited by 44 publications

References 9 publications

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

Use of Human Dental Pulp and Endothelial Cell Seeded Tyrosine-Derived Polycarbonate Scaffolds for Robust in vivo Alveolar Jaw Bone Regeneration

The Effects of Dental Pulp Stem Cell Conditioned Media on the Proliferation of Peripheral Blood Mononuclear Cells

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