Study of the Histopathologic Effects of Probiotic Lactobacillus acidophilus in Exposure to E. coli O157: H7 in Zebrafish Intestine

Elahi, Seyedeh Shaghayegh Mirabdollah; Mirnejad, Reza; Kazempoor, Reza; Sotoodehnejadnematalahi, Fattah

doi:10.5812/ircmj.99400

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…The use of the two postbiotics thus resulted in a rearrangement of the GC functions towards production of acidic mucins to protect against bacterial translocation via fortification of the mucus barrier by modifying mucus viscosity and buffering capacity [44]. Several studies reported a positive effect of different strains of lactobacilli, either live or inactivated, on the number and the chemotype of GC in various animal species, including zebrafish, subjected to intestinal challenges [45][46][47]. Interestingly, exopolysaccharides extracted from a strain of L. plantarum NCU116 have been shown to enhance GC differentiation and to promote the expression of the muc2 gene of colonic cells of a mouse subjected to a colitis challenge [48], emphasizing that bacterial surface molecules are involved in this effect.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Zebrafish (Danio rerio) Intestinal Mucosal Barrier Function Fed Different Postbiotics and a Probiotic from Lactobacilli

Rawling,

Schiavone,

Mugnier

et al. 2023

Microorganisms

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It is generally accepted that microbes play a critical role in maintaining gut barrier function, making them ideal to target in order to mitigate the effects of intestinal diseases such as inflammatory bowel disease with specialist supplementations such as probiotic or postbiotic preparations. In this study, specific strains of Lactobacillus helvictus both live and inactivated and Lactobacillus plantarum inactivated were fed to zebrafish at an inclusion level of 6 × 106 cells/g in order to assess the effects on gut barrier function and protection. Taken together, our results indicate that dietary administration of pro- or postbiotics strengthens the gut barrier function and innate immunity of healthy zebrafish in a strain-specific and process-dependent way. With some differences in the response intensity, the three treatments led to increased intestinal villi length and proportion of IELs, reinforcement of the GC population and up-regulated expression of biomarkers of AMP production and tight junction zona-occludin 2a (zo-2a). In addition, LPPost had an impact on the adaptive immune response, and we hypothesized that it conferred the potential to drive Th17/ILC3 immunity, as suggested by its effect on the gene expression of il22, of different AMPs, and the expression of zo2a. Moreover, LPPost showed the potential to drive Th1/ILC1-like immunity, with a higher percentage of CD8+ cells and higher ifnγ gene expression. In summary, the use of inactivated Lactobacilli species in this study represented a promising strategy for improving barrier function and regulating the immune fate of the intestinal mucosa in a strain-specific way.

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Section: Discussionmentioning

confidence: 99%

Modulation of Zebrafish (Danio rerio) Intestinal Mucosal Barrier Function Fed Different Postbiotics and a Probiotic from Lactobacilli

Rawling,

Schiavone,

Mugnier

et al. 2023

Microorganisms

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“…E. coli O157: H7 and E. coli O127: H6 adhesion to epithelial cells monolayers [82,83]. Unfortunately, probiotics need to be further studied to evaluate their efficacy as anti-biofilm against pathogenic E. coli.…”

Section: Novel Engineered Probiotics/postbiotics/synbioticsmentioning

confidence: 99%

Escherichia coli (E. coli) Resistance against Last Resort Antibiotics and Novel Approaches to Combat Antibiotic Resistance

Elshimy¹

2023

Escherichia Coli - Old and New Insights

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An important feature complicating the treatment of infections caused by E. coli is the increase in resistance to different antibiotics, even to last resort antibiotics. When resistant bacteria spread to the community, resistance creates comprehensive infection control issues, increasing morbidity for non-hospitalized patients of all ages and sexes. New resistance mechanisms are constantly being described, and new genes and vectors of transmission are identified on a regular basis. This chapter reviews different mechanisms of E. coli resistance against different classes of last resort antibiotics such as fosfomycin, nitrofurantoin, and polymixins. In addition, E. coli vaccines, epidemiology, and novel approaches to combat antibiotic resistance will be discussed throughout the chapter. In the age of antibiotic resistance and precise microbial genome engineering, many new strategies are now being used to combat multidrug-resistant bacteria, hoping to be our end game weapon. These strategies include CRISPR-Cas antimicrobials, nanobiotics, phage therapy, and probiotics, which promise to have a substantial impact on the way we treat diseases in the future, as we will discuss in the chapter.

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Probiotics in the prevention and treatment of infections with Helicobacter pylori, Enterohemorrhagic Escherichia coli, and Rotavirus

Das

Ragavan

Mandal³

2022

Probiotics in the Prevention and Management of Human Diseases

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Study of the Histopathologic Effects of Probiotic Lactobacillus acidophilus in Exposure to E. coli O157: H7 in Zebrafish Intestine

Cited by 7 publications

References 26 publications

Modulation of Zebrafish (Danio rerio) Intestinal Mucosal Barrier Function Fed Different Postbiotics and a Probiotic from Lactobacilli

Modulation of Zebrafish (Danio rerio) Intestinal Mucosal Barrier Function Fed Different Postbiotics and a Probiotic from Lactobacilli

Escherichia coli (E. coli) Resistance against Last Resort Antibiotics and Novel Approaches to Combat Antibiotic Resistance

Probiotics in the prevention and treatment of infections with Helicobacter pylori, Enterohemorrhagic Escherichia coli, and Rotavirus

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