Study of the ethiological agent of gnathostomosis in Nayarit, Mexico

León‐Régàgnon, Virginia; Osorio-Sarabia, David; Garcı́a-Prieto, Luis; Akahane, H.; Lamothe-Argumedo, Rafael; Koga, Masataka; Messina-Robles, Maud; Álvarez-Guerrero, Cesar

doi:10.1016/s1383-5769(02)00014-4

Cited by 36 publications

(31 citation statements)

References 5 publications

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“…Our results showed that amplification fragments of ITS-1 and ITS-2 were 900 and 600 basepairs, respectively, which indicated that ADVL3 obtained were those of G. binucleatum. 28 Identity of parasites used in this study was confirmed by alignment of the 421-basepair fragment corresponding to ITS-2, which showed 100% identity with reported sequences of G. binucleatum 25,28 and 99.76% identity with sequence from the larva isolated from a biopsy specimen. 25 The protein profile of the crude extract of ADVL3 of G. binucleatum showed bands ranging from 15 kD to 190 kD; prominent bands ranged from 35 kD to 45 kD (Figure 1).…”

Section: Resultssupporting

confidence: 57%

“…21,22,29 However, in the Pacific region of Mexico, species of Gnathostoma other than G. spinigerum and G. doloresi are responsible for the disease, specifically G. binucleatum. 25 Thus, antigens and immune responses observed in patients infected with other species of Gnathostoma could be different for those observed with patients infected with G. binucleatum.…”

Section: Discussionmentioning

confidence: 96%

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Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano‐Zaragoza¹,

Durán‐Avelar²,

Messina-Robles³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked freshwater fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting. Our results do not show differences in the antigens recognized by IgM and IgE. However, we found that the specific humoral immune response is caused mainly by IgG, specifically IgG4. We found that 43%, 65.2%, 54.1%, and 26.3% of the patients recognize the 37-kD, 33-kD, 31-kD, and 24-kDa antigens, suggesting that the 33-kD antigen is the immunodominant antigen of G. binucleatum.

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Section: Resultssupporting

confidence: 57%

Section: Discussionmentioning

confidence: 96%

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano‐Zaragoza¹,

Durán‐Avelar²,

Messina-Robles³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…The alignment of the 421 bp product of ITS-2 showed 100 % identity with the sequences reported for G. binucleatum (Almeyda-Artigas et al, 2000;León-Regagnon et al, 2002) and 99.76% with the nucleotide sequence of a parasite of the same species obtained by biopsy (Almeyda-Artigas et al, 2000), indicating that the larvae used to obtain the ESP were indeed G. binucleatum. The ESP showed proteolytic activity against bovine gelatin at both pH 8 and pH 4.5.…”

Section: Resultsmentioning

confidence: 64%

Proteases secreted by Gnathostoma binucleatum degrade fibronectin and antibodies from mammals

Vibanco-Pérez¹,

Durán‐Avelar²,

Zambrano‐Zaragoza³

et al. 2015

Helminthologia

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SummaryGnathostomiasis is a prevalent zoonosis in humans in some regions of the world. The genus Gnathostoma is considered an accidental parasite for humans; G. binucleatum is the endemic species in Nayarit, Mexico. This work was designed to determine the proteolytic activity of the excretory-secretory products (ESP) of advanced third-stage larvae (ADVL3) of Gnathostoma binucleatum against human fibronectin and antibodies from human and sheep. Our findings showed protease activity against human fibronectin as well as sheep and human gamma globulins of the ESP at molecular weights of 80 and 56 kDa. The proteases found in the ESP of G. binucleatum are thus candidate molecules for consideration as pathogenic elements, owing to the fact that they destroy proteins of the host tissue, which probably allows them to migrate through those tissues and degrade molecules involved in the humoral immune response.

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“…and the disease is endemic in Asia and the Americas, [1][2][3][4] and in returned travelers who had visited the endemic areas of this harmful parasite. 5,6 Humans acquire infection by consuming raw or undercooked meat, i.e., freshwater fish, frogs, chicken, etc., which harbor Gnathostoma advanced third-stage larvae (AL3).…”

Section: Introductionmentioning

confidence: 99%

Application of Recombinant Gnathostoma spinigerum Matrix Metalloproteinase-Like Protein for Serodiagnosis of Human Gnathostomiasis by Immunoblotting

Janwan¹,

Intapan²,

Yamasaki³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Matrix metalloproteinase (MMPs) is the extracellular zinc-dependent endopeptidase and is secreted for degrading extracellular matrix molecules of host tissues. A cDNA encoding MMP-like protein of Gnathostoma spinigerum larvae was amplified by reverse transcription-polymerase chain reaction, and was cloned into a prokaryotic expression vector, and expressed in Escherichia coli. Total immunoglobulin G class (total IgG) antibody responses to the recombinant MMP-like protein were analyzed by immunoblot diagnosis of human gnathostomiasis. Serum samples from proven and clinically suspected cases of gnathostomiasis, other parasitic diseases patients, and from healthy volunteers were tested. The immunoblotting gave high sensitivity (100%) and specificity (94.7%). Positive and negative predictive values were 85.4% and 100%, respectively. Recombinant MMP-like protein can be used as a diagnostic antigen and potentially replace native parasite antigens to develop a gnathostomiasis diagnostic kit.

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Study of the ethiological agent of gnathostomosis in Nayarit, Mexico

Cited by 36 publications

References 5 publications

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Proteases secreted by Gnathostoma binucleatum degrade fibronectin and antibodies from mammals

Application of Recombinant Gnathostoma spinigerum Matrix Metalloproteinase-Like Protein for Serodiagnosis of Human Gnathostomiasis by Immunoblotting

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