Study of the enzymatic digestive profile in three species of Mediterranean sea urchins

Trenzado, Cristina E.; Hidalgo, F.; Villanueva, Daniel; Furné, M.; Díaz-Casado, María E.; Merino, R.; Sanz, A.

doi:10.1016/j.aquaculture.2012.03.027

Cited by 28 publications

(24 citation statements)

References 43 publications

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“…A small set of previously reported genes of echinoderms involved in metabolic processes, immune response, reproduction, and speciation, such as digestive enzymes, immune genes, potential pheromones, and proteins involved in gamete recognition and gametogenesis, here termed as 'target genes', were selected based on the literature available (Suzuki 1995;Kamei & Glabe 2003;Shah et al 2003;Smith et al 2006;Trenzado et al 2012;Hart 2013;Novo et al 2013;Li et al 2014; among others), and sequences obtained via local BLAST (tblastx engine) against the de novo assemblies using GENEIOUS v. 8.03 (if not recovered from the BLAST results of the de novo assemblies). Expression of target genes was also inspected for significant differences as obtained before.…”

Section: Selection Of Target Genesmentioning

confidence: 99%

“…Digestive. Arbacia lixula has manifested a diverse digestive enzyme pool and protease activity compared to the other most common sea urchins in the Mediterranean Sea (Trenzado et al 2012). Interestingly, an alpha-amylase, a trypsin enzyme (neurotrypsin), and nine lipases were differentially expressed in the digestive (Appendix S7, Supporting information).…”

Section: Differences Between Tissuesmentioning

confidence: 99%

“…), manifested by a more diverse digestive enzyme pool as compared to other Mediterranean Sea urchins (Trenzado et al . ). Due to its trophic activity, the species plays a principal role in producing and maintaining Mediterranean barrens (Bonaviri et al .…”

Section: Introductionmentioning

confidence: 97%

“…It is an amphi-Atlantic species described as a recent thermophilous colonizer of the Mediterranean Sea that spread throughout this basin during a warm period of the Pleistocene (Wangensteen et al 2012). A. lixula possesses a wide trophic plasticity (Wangensteen et al 2011), manifested by a more diverse digestive enzyme pool as compared to other Mediterranean Sea urchins (Trenzado et al 2012). Due to its trophic activity, the species plays a principal role in producing and maintaining Mediterranean barrens (Bonaviri et al 2011), a trend that could be worsening in the near future due to foreseeable climatic changes by releasing the constraint imposed to larval development at low water temperature (Privitera et al 2011;Wangensteen et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the transcriptome and gene expression of four different tissues in the ecologically relevant sea urchin Arbacia lixula using RNA‐seq

Pérez‐Portela

Turon

Riesgo

2015

Molecular Ecology Resources

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The sea urchin Arbacia lixula is a keystone species in Mediterranean ecosystems that drive landscape changes in littoral communities. However, genomic information available for the whole order Arbacioida is very limited. Using RNA-seq techniques, we have characterized the transcriptome of four different tissue types in A. lixula: the 'somatic' tissues (coelomocytes and digestive tissue) and the 'reproductive' tissues (ovary and testis), from two replicated cDNA libraries for each sample. Additionally, we performed a de novo assembly to build the 'reference' transcriptome, pooling reads of the four tissues, to analyse the differential expression (DE) in pairwise comparisons between tissues. The complete de novo assembly yielded 186,084 transcripts, with a sequence size limit of 100 nt, being 31% of them spliced isoforms. Approximately 21% of the transcripts had blast hits against proteins of metazoans (E < 10(-5) ), being less than 2.2% functionally annotated. Between coelomocytes and digestive, 30,794 transcripts showed DE (~11.8% of them with blast hit), and 19,567 transcripts did so between testis and ovary (~28.5% of them with blast hit). Major GO-term categories upregulated in somatic tissues were those related to muscle contraction and energy generation in digestive, and lipid metabolism associated with immune response in coelomocytes. Between reproductive tissues, the major upregulated GO categories were related to energy generation in testis, and negative regulation of nucleotide metabolism in ovary. We particularly screened for a collection of target genes in each tissue because of their relevance for further studies on evolution and adaptation of echinoids.

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Section: Selection Of Target Genesmentioning

confidence: 99%

Section: Differences Between Tissuesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of the transcriptome and gene expression of four different tissues in the ecologically relevant sea urchin Arbacia lixula using RNA‐seq

Pérez‐Portela

Turon

Riesgo

2015

Molecular Ecology Resources

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“…Digestive enzymatic profiles have been used to assess feeding habits, digestive capacity or nutritional condition of some marine invertebrates such as lobsters (Johnston 2003;Johnston et al 2004), crabs (Rotllant et al 2010), bivalves (Labarta et al 2002) and abalone (Takami et al 1998;Takami and Kawamura 2003). A range of digestive enzymes have been detected in the guts of adult sea urchins, including amylase, lipase, protease, trypsin, chymotrypsin, CMCase and laminarinase (Trenzado et al 2012). However, there are no studies on qualitative and quantitative changes in the enzymes with sea urchin growth, especially during early life.…”

Section: Introductionmentioning

confidence: 98%

Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

et al. 2015

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ingested diets were observed for urchins >3 mm in TD. Our results suggest that the primary nutrient source of S. intermedius shifts from microalgae to macroalgae at ≈6 mm TD, and that macroalgae digestion efficiency in urchins ≥3 mm TD gradually improves because of activation of the enzymes. Highly activated enzymes enable sea urchins to efficiently digest nutrients stored in the bodies of macroalgae, allowing the primary nutrient source of S. intermedius to shift from microalgae to macroalgae.

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Tooth Mg/Ca ratios and Aristotle's lantern morphometrics reflect trophic types in echinoids

Vafidis,

Varkoulis,

Zaoutsos

et al. 2024

Ecology and Evolution

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Several studies have inferred the ecological significance regarding the morphometrics of Aristotle's lantern and the mechanical properties of magnesium in echinoid teeth. This study attempts to combine these aspects, connecting them to the trophic habits of three native and an invasive echinoid in the Eastern Mediterranean Sea. Spatiotemporal data from the central and southern Aegean Sea were obtained, regarding the relative size of lanterns and demi‐pyramids of Arbacia lixula, Paracentrotus lividus, Sphaerechinus granularis, and the invasive echinoid Diadema setosum and the Mg/Ca ratios of four zones on the tooth cross‐section. Since environmental factors affect the examined factors, data for temperature, salinity, and concentration of chlorophyll‐a were included in a principal component analysis. A. lixula and P. lividus presented intraspecific differences in the relative size of the lantern and demi‐pyramid, while S. granularis and D. setosum exhibited variation in the elongation index. Differences in the Mg/Ca ratios were observed for all species although in different zones. Temperature appears to be related to all Mg/Ca zones except for the stone part, while the elongation index showed an inverse trend to all other morphometric parameters. The results of the PCA for the four species on the spatiotemporal level exhibited a distinction of individuals with season but not species, except for A. lixula, an omnivore with a carnivorous tendency, which was clearly separated from the herbivorous species. Using hierarchical clustering on the principal components it was evident that the three native species occupy different clusters, but when D. setosum was added, it shared the same cluster with S. granularis. This might infer similar feeding preferences, specifically for coralline algae, which might lead to a swift in the ecological equilibrium in regions, where D. setosum is found, either by affecting habitat type, or by restricting the distribution of S. granularis as was previously observed with Diadema africanum.

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Study of the enzymatic digestive profile in three species of Mediterranean sea urchins

Cited by 28 publications

References 43 publications

Characterization of the transcriptome and gene expression of four different tissues in the ecologically relevant sea urchin Arbacia lixula using RNA‐seq

Characterization of the transcriptome and gene expression of four different tissues in the ecologically relevant sea urchin Arbacia lixula using RNA‐seq

Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

Tooth Mg/Ca ratios and Aristotle's lantern morphometrics reflect trophic types in echinoids

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