Study of the Effect of Hyperbaric Oxygen Therapy on the Viability of Dorsal Cutaneous Flaps in Tobacco-Exposed Rats

Camargo, Cristina Pires; Pfann, R. Z.; Kubrusly, Márcia Saldanha; Silva, M. F. R.; Guimarães, Eliane Tigre; Leite, Mariana Silva Moreira; Saldiva, Paulo Hilário Nascimento; Gemperli, Rolf

doi:10.1007/s00266-020-01677-3

Cited by 3 publications

(1 citation statement)

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“…10 Experimental therapies including the use of antioxidants, vasodilators, anti-inflammatory drugs, and hyperbaric oxygen led to a decrease in necrosis rates. [11][12][13][14][15][16] Alternatively, therapeutic angiogenesis through the application of pro-angiogenic factors, such as Platelet-derived growth factor (PDGF), and Vascular Endothelial Growth Factor (VEGF) has been demonstrated to improve the survival of compromised flaps by improving tissue perfusion. 10,[17][18][19][20][21] The use of platelet-rich plasma (PRP) resulted in an increase in flap survival of 20% by inducing angiogenesis and reducing the inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

The secretome of stressed peripheral blood mononuclear cells increases tissue survival in a rodent epigastric flap model

Hacker

Mittermayr

Traxler

et al. 2020

Bioengineering & Transla Med

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Reconstructive surgery transfers viable tissue to cover defects and to restore aesthetic and functional properties. Failure rates after free flap surgery range from 3 to 7%. Comorbidities such as diabetes mellitus or peripheral vascular disease increase the risk of flap failure up to 4.5-fold. Experimental therapeutic concepts commonly use a monocausal approach by applying single growth factors. The secretome of γ-irradiated, stressed peripheral blood mononuclear cells (PBMCsec) resembles the physiological environment necessary for tissue regeneration. Its application led to improved wound healing rates and a twofold increase in blood vessel counts in previous animal models. We hypothesized that PBMCsec has beneficial effects on the survival of compromised flap tissue by reducing the necrosis rate and increasing angiogenesis. Surgery was performed on 39 male Sprague-Dawley rats (control, N = 13; fibrin sealant, N = 14; PBMCsec, N = 12). PBMCsec was produced according to good manufacturing practices (GMP) guidelines and 2 ml were administered intraoperatively at a concentration of 2.5 × 10 7 cells/ml using fibrin sealant as carrier substance. Flap perfusion and necrosis (as percentage of the total flap area) were analyzed using Laser Doppler Imaging and digital image planimetry on postoperative days 3 and 7. Immunohistochemical stainings for von Willebrand factor (vWF) and Vascular Endothelial Growth Factor-receptor-3 Wolfgang Michlits and Hendrik J. Ankersmit contributed equally.

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