Study of the effect of some organic solvents on the activity and stability of glucose oxidase

Vasileva, N.; Godjevargova, Tzonka

doi:10.1016/j.msec.2004.03.001

Cited by 30 publications

(18 citation statements)

References 14 publications

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“…Other authors have found that improving the stability of the enzyme can be achieved only at low concentrations of organic solvent [37][38][39]. By increasing the concentration of organic solvent, however, was observed inactivation [31,40], caused mainly from reversible conformational changes in the protein molecules. The use of immobilized enzymes makes it possible to enhance the stability of the enzymes at higher concentrations of organic solvent.…”

Section: Effect Of Modified Agent On the Proprieties Of Immobilized Ementioning

confidence: 99%

“…It was found that they can be improved by adding various components to the reaction medium, such as sugars, polyoxyethylene, ethyl cellulose, polyols, amino acids, hydrophobic organic solvents and others [26][27][28][29][30][31]. The latter form multiple hydrogen bonds with amino acid residues of the enzyme to prevent it from denaturation [32].…”

Section: Effect Of Modified Agent On the Proprieties Of Immobilized Ementioning

confidence: 99%

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Immobilization of β-galactosidase on modified polypropilene membranes

Vasileva

Iotov

Ivanov

et al. 2012

International Journal of Biological Macromolecules

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Section: Effect Of Modified Agent On the Proprieties Of Immobilized Ementioning

confidence: 99%

Section: Effect Of Modified Agent On the Proprieties Of Immobilized Ementioning

confidence: 99%

Immobilization of β-galactosidase on modified polypropilene membranes

Vasileva

Iotov

Ivanov

et al. 2012

International Journal of Biological Macromolecules

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“…The immobilization prevents, to a great extent, the protein-protein interaction which leads to aggregation of protein molecules and, thus, to irreversible deactivation. 8 The thermal stability of enzymes is important for the preparation of biosensors based on immobilized enzymes, which have applications in monitoring food and fermentation processes. Glucose oxidase is the enzyme used in glucose biosensors and increasing its thermal stability is relevant to its practical application.…”

Section: Introductionmentioning

confidence: 99%

Preparation of glucose oxidase immobilized in different carriers using radiation polymerization

Atia¹,

El-Batal

2005

J of Chemical Tech & Biotech

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Glucose oxidase (EC 1.1.3.4) was immobilized on different polymeric materials using different immobilization techniques (entrapping by γ -irradiation, and covalent binding using epichlorohydrin). Studies were carried out to increase the thermal stability of glucose oxidase (GOD) for different applications. The activity and stability of the resulting biopolymers have been compared with those of free GOD. The effect of different polyvinyl alcohol/polyacrylamide (PVA/PAAm) compositions of the copolymer carrier on the enzymatic activity of the immobilized GOD was studied. The maximum enzymatic activity was obtained with the composition ratio of PVA/PAAm of 60:40. The behaviour of the free and immobilized enzyme was analysed as a function of pH. A broadening in the pH profile (5.5-8) was observed for immobilized preparations. The activity and stability of the resulting biopolymers produced by immobilization of GOD onto different carriers have been compared, in both aqueous and organic media, with those of the free GOD. The enzyme's tolerance toward both heat and organic solvent was enhanced by immobilization onto polymers. The addition of different concentrations of organic solvents (10-50%, v/v) to the enzyme at higher temperature (60 • C) was found to stabilize the enzyme molecule. The strongest stabilizing effect on the enzymatic activity was achieved at a concentration of 10%.

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“…[16][17][18] In these systems, the incorporation of GOx in the presence of glucose (reducing agent) to the reaction mixture leads to the reduction of molecular oxygen, generating hydrogen peroxide. Several attractive features of this approach include low cost, efficient enzymatic activity in the presence of organic solvents, 24 and exceedingly mild polymerization conditions. Extension of this system in combination with Cu-mediated ATRP to SI-CRP was recently reported by Zauscher and coworkers, 25 while ATRP and RAFT systems based on select photocatalysts have been used to achieve oxygen tolerance for polymer brush synthesis.…”

mentioning

confidence: 99%

Surface‐initiated PET‐RAFT polymerization under metal‐free and ambient conditions using enzyme degassing

Seo

Discekici

Zhang

et al. 2019

Journal of Polymer Science

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An open‐to‐air method for the efficient synthesis of surface‐tethered polymer brushes based on photoinduced electron transfer‐reversible addition‐fragmentation chain transfer (PET‐RAFT) polymerization is reported. Key to this approach is an enzyme‐assisted strategy using glucose oxidase to facilitate the in situ removal of oxygen during the polymerization process. Control experiments in the absence of glucose oxidase confirm the importance of enzymatic deoxygenation for successful polymerization of a variety of acrylamide, methacrylate, and acrylate monomers. In accordance with controlled polymerization kinetics, a linear increase in brush height as a function of irradiation time for a range of light intensities is demonstrated. Importantly, the use of light to mediate growth and the inherent monomer versatility of PET‐RAFT allow for the facile fabrication of well‐defined polymer brushes under aqueous conditions. © 2019 Wiley Periodicals, Inc. J. Polym. Sci. 2020, 58, 70–76

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Study of the effect of some organic solvents on the activity and stability of glucose oxidase

Cited by 30 publications

References 14 publications

Immobilization of β-galactosidase on modified polypropilene membranes

Immobilization of β-galactosidase on modified polypropilene membranes

Preparation of glucose oxidase immobilized in different carriers using radiation polymerization

Surface‐initiated PET‐RAFT polymerization under metal‐free and ambient conditions using enzyme degassing

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