Study of the DPPH-scavenging activity: Development of a free software for the correct interpretation of data

Locatelli, Monica; Gindro, Roberto; Travaglia, Fabiano; Coïsson, J. D.; Rinaldi, Maurizio; Arlorio, Marco

doi:10.1016/j.foodchem.2008.10.035

Cited by 160 publications

(87 citation statements)

References 35 publications

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“…The scavenging activity of DPPH radicals was calculated according to the following equation: DPPH radical scavenging activity (%)ϭ(1Ϫabsorbance of sample/absorbance of control)ϫ100. 17) …”

Section: Methodsmentioning

confidence: 99%

Halioxepine, a New Meroditerpene from an Indonesian Sponge Haliclona sp.

Trianto

Hermawan

Voogd

et al. 2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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Extensive investigations on marine natural products have shown that marine sponges are nearly inexhaustible sources of new secondary metabolites.1) From a structural viewpoint, various classes of molecules including macrolides, 2) polypeptides, 3) terpenes, 4) steroids, 5) alkaloids, 6) and others 7) have been isolated, and a number of different modes of biological activity have been reported. The sponges belonging to the genus Haliclona are also prolific sources of unique molecules such as manzamines, 8) haliclonamines, 9) haliclonamides, 10) halipeptins, 11) and others. In our continuing search for new cytotoxic metabolites from Indonesian sponges, 12,13) we collected a small sponge belonging to Haliclona at Baubau, Indonesia. After the 1 H-NMR spectrum of a methanolic extract from the sponge indicated the presence of a unique molecule, we examined the chemical contents. In this note we describe the isolation, structure characterization, and bioactivity of the new compound, which we named halioxepine (1). Results and DiscussionThe sponge (45 g, wet) was soaked in MeOH to provide a crude extract, whose 1 H-NMR spectrum showed the presence of a unique metabolite as one of principal components. The extract was triturated with CH 2 Cl 2 to provide a lipophilic fraction (136.5 mg) followed by purification on silica HPLC to give compound 1 (25.3 mg).Compound 1 Chemical investigations on a sponge Haliclona sp. found a meroditerpene 1 having a new carbon skeleton. By analyzing spectroscopic data, the structure was elucidated to comprise a substituted hydroquinone, a tetrahydrooxepine, and a cyclohexene, and these components were united with C1 and C2 units. Compound 1 showed moderate cytotoxicity against NBT-T2 cells with IC 50 4.8 m mg/ml and also antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC 50 3.2 m mg/ml.

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Section: Methodsmentioning

confidence: 99%

Halioxepine, a New Meroditerpene from an Indonesian Sponge Haliclona sp.

Trianto

Hermawan

Voogd

et al. 2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…The DPPH radical scavenging assay was performed according to the method reported by Locatelli et al [22]. Briefly, 700 μL of sample (grape extracts diluted 1:500 with MeOH) or MeOH (control) was added to the same volume of methanolic solution of DPPH · (100 μM).…”

Section: Antioxidant Activity (Dpph Radical Scavenging Assay)mentioning

confidence: 99%

Phenolic composition of Nebbiolo grape (Vitis vinifera L.) from Piedmont: characterization during ripening of grapes selected in different geographic areas and comparison with Uva Rara and Vespolina cv.

Locatelli

Travaglia

Coı̈sson

et al. 2015

Eur Food Res Technol

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“…Although, in vitro methods used in assessing radical scavenging activity are artificial radicals and do not reproduce in vivo situation, they are however, useful in the evaluation of the antioxidant activity in a rapid, easy, and inexpensive way. Besides, it is important to note that, a valid in vitro assay is an invaluable tool for clinical studies if it is combined with bioavailability data and valid oxidative stress biomarker assays [55,56].…”

Section: Antioxidant Activity and Their Correlations To The Polyphenomentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Selected Plants Used by Populations From Juruena Valley, Legal Amazon, Brazil

Silva

Arunachalam

Miyajima

et al. 2017

Int J Pharm Pharm Sci

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Objective: The purpose of this study was to evaluate selected Brazilian plants from Juruena valley region of Mato Grosso, for their in vitro antimicrobial and antioxidant activities. Methods:The powder obtained from different parts of the twenty-six (26) plants were macerated in hydroethanolic solution to obtain the extracts. The hydroethanolic extracts were tested for their in vitro antimicrobial activity by determining the MIC using broth microdilution. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, ferric reducing antioxidant power (FRAP) and nitric oxide (NO) methods were used for the determination of antioxidant activities. Correlation between classes of secondary metabolites and antioxidant activity was assessed.Results: Phanera glabra extract (HEPg) showed broad antibacterial spectrum, presenting the best activity against Klebsiella pneumoniae. Hevea microphylla extract (HEHm) presented a narrow spectrum of antibacterial activity with strong effect against Shigella flexneri. The only plant with broad spectrum antifungal activity was Bertholletia excelsa (HEBe), with moderate activity against strains of Aspergillus and Candida. The following extracts were prominent regarding their activities in the DPPH and FRAP assays-HEBe, Cariniana rubra (HECr) and in the FRAP assay alone, Cedrela odorata (HECo) and HEPg. None of the extracts was active in the NO assay. A significant association was observed between DPPH activity and the total phenolic contents. Conclusion:Our results justified the use of some of the investigated plants in the Brazilian ethnomedicine. The antibacterial activities of these plants are bacteriostatic in nature. These findings support that a number of investigated plants could be a valuable source of new antioxidant and antimicrobial compounds that can potentially deliver novel mechanisms of actions. However, further studies are required.

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Study of the DPPH-scavenging activity: Development of a free software for the correct interpretation of data

Cited by 160 publications

References 35 publications

Halioxepine, a New Meroditerpene from an Indonesian Sponge Haliclona sp.

Halioxepine, a New Meroditerpene from an Indonesian Sponge Haliclona sp.

Phenolic composition of Nebbiolo grape (Vitis vinifera L.) from Piedmont: characterization during ripening of grapes selected in different geographic areas and comparison with Uva Rara and Vespolina cv.

Antimicrobial and Antioxidant Activities of Selected Plants Used by Populations From Juruena Valley, Legal Amazon, Brazil

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