Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

Singh, S. B.; Verma, Sanjay Kumar

doi:10.1007/s13659-012-0069-1

Cited by 31 publications

(18 citation statements)

References 18 publications

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“…26 . These data correspond to the piperidine alkaloid juliprosopine, previously isolated from another species of the same genus, P. juliflora (Dos Santos et al, 2013;Nakano et al, 2004;Singh and Verma, 2012). The second majority peak at 7.72 minutes showed a molecular ion at 644.973 m/z [M + H] + and a main ion (base peak) at 323.145 m/z [M + 2H] 2+ .…”

Section: Hilic Lc-esi-ms Analysissupporting

confidence: 78%

Effect of Prosopis ruscifolia on lipid profile in alloxan-induced hyperglycemic mice and chemical characterization of alkaloid and flavonoid fractions

2019

J App Pharm Sci

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Diabetes mellitus interferes with the metabolism of carbohydrates, causing chronic hyperglycemia. Dyslipidemia in diabetes is a condition that leads to cardiovascular disease. This study was aimed to evaluate the effect of hydroalcoholic Prosopis ruscifolia (Pr) leaves extract on hyperglycemia and lipid profile in normo-and hyperglycemic mice. Mice hyperglycemia was induced by alloxan, animals were treated with Pr (50, 100, 200 mg/kg, p.o., 28 days). Fasted blood glucose level on days 7, 14, 21, and 28 was determined. Blood glucose remained within the normal range in the groups of normoglycemic animals treated with Pr. In the hyperglycemic animals, 100 mg/kg of Pr extract reduced the glycemia, this effect became markedly evident since day 7, until the end of experimental period (p < 0.0001), the total reduction reached was 60%. The lipid profile of normal and hyperglycemic mice was evaluated with 100 mg/kg, on day 28. A non-significant increase in total cholesterol and low density cholesterol, in hyperglycemic animals treated with vehicle, and a statistically significant increase (p < 0.0001) in the level of triglyceride and very low density cholesterol (VLDL) level (p < 0.0001) in normoglycemic animals treated with Pr, compared to the control group were denoted. This could indicate that Pr has a stimulating action on insulin secretion, since hyperinsulinemia is also associated with an increase in the quantity of atherogenic particles of VLDL cholesterol and triglycerides. The coronary risk index and the atherogenic index of hyperglycemic animals treated with Pr showed a reduction compared with the untreated hyperglycemic ones. The presence of three piperidine alkaloids, juliprosopine, 3''''-Oxo-juliprosopine, and julifloridine, previously isolated from P. juliflora, was confirmed. Also, the presence of the flavonoid quercetin was detected in this plant. Those compounds are strong candidates presumably responsible for imparting the effect on glycemia and lipid profile reported here.

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Section: Hilic Lc-esi-ms Analysissupporting

confidence: 78%

Effect of Prosopis ruscifolia on lipid profile in alloxan-induced hyperglycemic mice and chemical characterization of alkaloid and flavonoid fractions

2019

J App Pharm Sci

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“…The desorption/ionization methods for mass spectrometry had been technologically advanced for chemical identification over the past decades [ 15 ]; however, the characterization of plant extract using mass spectrometry is relatively difficult due to the lack of reliable extraction procedures [ 13 , 14 , 16 ]. Quadruple time-of-flight liquid chromatography-mass spectroscopy (Q-TOF LC/MS) technology provides an outstanding separation of major and trivial constituents of natural product extracts along with increasing selectivity and avoiding false-positive findings [ 17 , 18 ]. This high level of performance allows the elemental composition of each m/z value to be accurately calculated using the software.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic, Antimicrobial, and Molecular Profiling of Selected Medicinal Plants

Aryal

Niraula

Khadayat

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Natural products have been the center of attraction ever since they were discovered. Among them, plant-based natural products were popular as analgesics, anti-inflammatory, antidiabetic, and cosmetics and possess widespread biotechnological applications. The use of plant products as cosmetics and therapeutics is deep-rooted in Nepalese society. Although there are few ethnobotanical studies conducted, extensive research of these valuable medicinal plants has not been a priority due to the limitation of technology and infrastructure. Here, we selected 4 traditionally used medicinal plants to examine their bioactive properties and their enzyme inhibition potential. α-Glucosidase and α-amylase inhibitory activities were investigated using an in vitro model followed up by antioxidant and antimicrobial activities. The present study shows that ethyl acetate fraction of Melastoma melabathrium (IC50 9.1 ± 0.3 µg/mL) and water fraction Acacia catechu (IC50 9.0 ± 0.6 µg/mL) exhibit strong α-glucosidase inhibition. Likewise, the highest α-amylase inhibition was shown by crude extracts of Ficus religiosa (IC50 29.2 ± 1.2 µg/mL) and ethyl acetate fractions of Shorea robusta (IC50 69.3 ± 1.1 µg/mL), and the highest radical scavenging activity was shown by F. religiosa with an IC50 67.4 ± 0.6 µg/mL. Furthermore, to identify the metabolites within the fractions, we employed high-resolution mass spectrometry (LC-HRMS) and annotated 17 known metabolites which justify our assumption on activity. Of 4 medicinal plants examined, ethyl acetate fraction of S. robusta, ethyl acetate fraction of M. melabathrium, and water or ethyl acetate fraction of A. catechu extracts illustrated the best activities. With our study, we set up a foundation that provides authentic evidence to the community for use of these traditional plants. The annotated metabolites in this study support earlier experimental evidence towards the inhibition of enzymes. Further study is necessary to explore the clinical efficacy of these secondary molecules, which might be alternatives for the treatment of diabetes and pathogens.

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“…The results of the phytochemical prospecting of the aqueous extract of the dry powder of the mesquite leaf indicate the presence of the metabolites saponin, phenolic, flavonoids and alkaloids. The piperidine alkaloids, isolated from the leaves and fruits of this plant are referred to as the most important pharmacologically active compounds, having been tested in different biological systems, being attributed to the therapeutic activities conferred to the plant (SINGH et al, 2012). In addition, it also has anthelmintic activity (BATATINHA et al, 2011) and is an inhibitor of acetylcholinesterase and butyrylcholinesterase, with Ca 2+ channel blocking activity (CHOUDHARY et al, 2005) which can cause mortality to A. aegypti.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal effect of Prosopis juliflora on Aedes aegypti (Diptera: Culicidae)

Azevedo¹,

Oliveira²,

Moura³

et al. 2021

RICA

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The aim of this work was to evaluate the larvicidal effect of aqueous extracts of Prosopis juliflora on A. aegypti. Oviposition traps (ovitramps) were used to obtain the insect's eggs. The test was carried out at the Agricultural Entomology Laboratory of the Federal University of Cariri, Crato, Ceará, Brazil, with controlled temperature, humidity and photophase, using larvae in the 3º instar. The design used was completely randomized (DIC) with five doses (10, 20, 30, 40 and 50 g L-1) of the aqueous extract plus two control treatments (pyriproxifene at 0.01g L-1 and distilled water). The treatments were evaluated after 24, 48, 72, 96, 120, 144 and 168 h of exposure of the larvae, being considered dead those that did not react to the mechanical stimulus of a clamp. The doses of 40 and 50 g L-1 of P. juliflora are effective in the mortality of A. aegypti larvae after 48 h of exposure, with greater efficiency (100% mortality) after 120 h. The lowest doses (10 and 20 g L-1) have low mortality efficiency, while the dose of 30 g L-1 causes satisfactory mortality after 148 h of exposure. The aqueous extract of the leaves of P. Juliflora (in doses of 40 and 50 g L-1) is effective in controlling A. aegypti.

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Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

Cited by 31 publications

References 18 publications

Effect of Prosopis ruscifolia on lipid profile in alloxan-induced hyperglycemic mice and chemical characterization of alkaloid and flavonoid fractions

Effect of Prosopis ruscifolia on lipid profile in alloxan-induced hyperglycemic mice and chemical characterization of alkaloid and flavonoid fractions

Antidiabetic, Antimicrobial, and Molecular Profiling of Selected Medicinal Plants

Larvicidal effect of Prosopis juliflora on Aedes aegypti (Diptera: Culicidae)

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