Study of the distribution of microvessels in normal and pathologic prostate using an information-based similarity analysis

Santamarı́a, Luis; Ingelmo, Ildefonso; Ruiz, Jesus; Teba, Fernando

doi:10.1111/j.1365-2818.2011.03508.x

Cited by 3 publications

(5 citation statements)

References 31 publications

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“…Perhaps the rise in the blood PSA detected in prostate cancer can be partially explained by this factor. This increment of S V basal and increased permeability of the basement membrane also seems important when related to the microvessel density described in prostate cancer (Barth et al, 1997 ; Coloma et al, 2014 ; Santamaria et al, 2011 ). It may be a factor that intervenes in the spread of the tumor.…”

Section: Discussionmentioning

confidence: 84%

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Dimensional study of prostate cancer using stereological tools

2021

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Section: Discussionmentioning

confidence: 84%

“…In (c), the differences between CTR and CA are significant (p = 0.0001). The individual values are represented in CTR by empty circles and in CA by solid circles Santamaria et al, 2011). It may be a factor that intervenes in the spread of the tumor.…”

Section: Discussionmentioning

confidence: 99%

Dimensional study of prostate cancer using stereological tools

2021

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“…The capturing of images was made using a colour digital camera DP 70 (Olympus Corporation of the Americas, PA, USA) with a resolution of 12.5 mega-pixels, attached to an Olympus microscope fitted with a motorised stage controlled by the stereological software Cast-Grid (Stereology Software Package, Silkeborg, Denmark). This program controls the XY displacement of the microscope stage and allows the selection of fields to be studied by random systematic sampling after the input of an appropriate sampling fraction [27]. The result was a series of images from the two groups, sized 512 × 680 pixels.…”

Section: I) Estimation Of the Proliferative Index Of Epitheliummentioning

confidence: 99%

Quantitative Stereological Estimations of Structural Patterns of the Glandular Tree in Benign Hyperplasia of Prostate

Santamarı́a¹,

Ingelmo²,

Teba³

et al. 2016

OJPathology

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Background: Benign prostate hyperplasia (BPH) is the most common benign disease of human prostate. Currently BPH is associated with unregulated proliferation of connective tissue and glandular epithelium within the prostatic transition zone, and it has been described as relevant characteristic of BPH-the increase of the total number of cells, and not only an increase in cell size. To date, there are few studies on the quantitative morphology of glandular tree of BPH compared with normal prostate. The scarce investigations about this particular suggest that the glandular tree branches and expands as the hyperplastic transformation occurs in the prostate. Methods: To verify if this gland expansion and branching was similar to that occurs in the normal prostate, this study deals with the estimation of several stereological parameters as: labeling index for the proliferating cell nuclear antigen to quantify the rate of proliferation of prostate epithelium, average thickness of glandular epithelium, fraction of the volume occupied by the epithelium relative to the total prostate volume, connectivity density of prostate glands, to quantify the branching of prostate glands, and the average volume and the volume-weighted mean glandular volume of prostate acini to assess the mean size of the prostate acini and its variability. Results: All these estimates have been performed in prostate specific antigen immunostained sections from prostates of young men (controls) and in adenomectomy specimens from the adenofibromiomatous variety of BPH. Conclusion: We conclude that the epithelial proliferation is not the only factor intervening in the development of BPH. In addition, a more prolonged survival of epithelial * Corresponding author. L. Santamaría et al. 123 population, together with some degree of hypertrophy of acini expressed by the increase of volume fraction and thickness of acinar epithelium, is relevant in order to the growth and expansion of the BPH glandular tree that shows more abundant and heterogeneous acinar sprouts than in normal prostate.

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“…At that point, the strips were 9 mm long, on average. Therefore, the total length explored per section (five sections) and per case (10 cases) was 9 × 5 × 10 = 450 mm (for BPH cases, an appreciable percentage of the maximum specimen diameter) [21].…”

Section: Data Acquisitionmentioning

confidence: 99%

“…Recently, several studies have dealt with the estimation of measurements of either acinar or stromal parameters at the local level in normal and pathological human prostate, using second order stereological methods [20]. For example: The study of the distribution of microvessels in normal and pathological prostate [21], the distribution of cell nuclei populations in prostatic adenocarcinoma and PIN [22], the estimation of K function and isotropy of normal prostatic acini compared to cancer [23], and the local changes of glandular pattern in BPH compared to normal prostate [24]. In some of these studies, several discrepancies between global and local results were detected.…”

Section: Introductionmentioning

confidence: 99%

Structural Patterns of Immunoreactivity to Cytokeratin 18 in Normal Prostate and Benign Prostate Hyperplasia: Global and Local Differences

Santamarı́a¹,

Ingelmo²,

Teba³

2017

OJPathology

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Background: Benign prostate hyperplasia (BPH) is the most common benign disease of the human prostate. The comparison between global versus local changes in spatial patterns of pathological lesions provoked a growing interest in some fields such as neuropathology. To date, there is little data on this subject in prostatic pathology. Given the interest of local parameters to distinguish between normal and pathological structures, the present study will apply first and second order stereological tools to find out if the cytokeratin18 (ck18) immunoexpression shows relevant local changes in BPH compared to normal prostate, independently if global estimates were similar in both groups. Methods: To verify if the global and local changes in immunoexpression of ck18 are relevant to ascertain differences between normal (CTR) and BPH cases, the following parameters will be applied: Volume fraction of epithelium immunostained for ck18 (V V ck18), both in global and local estimates; dispersion indices of V V ck18; estimates of local variance of V V ck18 (positional and of scale) using wavelet analysis; and lacunarity analysis to measure the tissue heterogeneity. Then, the set of values from the parameters studied that show significant differences between CTR and BPH will be employed to perform stepwise linear discriminant analyses to determine if locally estimated parameters were able to classify accurately the cases in CTR and BPH groups. Results and Conclusions: The findings of the present study indicate that changes in the expression of ck18 by the hyperplastic prostatic epithelium are not homogeneous. This limits the use of a single biopsy based markers to predict biological behavior in BPH. On the other hand, the local changes in the expression of ck18 are more evident in terms of V V ck18 and its 26local variability, whereas other parameters that are useful in other pathologies, such as lacunarity, are less relevant In prostatic hyperplasia.

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Study of the distribution of microvessels in normal and pathologic prostate using an information-based similarity analysis

Cited by 3 publications

References 31 publications

Dimensional study of prostate cancer using stereological tools

Dimensional study of prostate cancer using stereological tools

Quantitative Stereological Estimations of Structural Patterns of the Glandular Tree in Benign Hyperplasia of Prostate

Structural Patterns of Immunoreactivity to Cytokeratin 18 in Normal Prostate and Benign Prostate Hyperplasia: Global and Local Differences

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