Study of the degradation of 5-azacytidine as a model of unstable drugs using a stopped-flow method and further data analysis with multivariate curve resolution

Argemí, Anna; Saurina, Javier

doi:10.1016/j.talanta.2007.05.053

Cited by 23 publications

(9 citation statements)

References 15 publications

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“…The spectrophotometric monitoring using a continuous-flow system has provided release profiles in an easy and simple way. Additionally, a liquid chromatographic method has been established to obtain the kinetic profiles of the delivery processes as well as to study the evolution of drug metabolite formation by hydrolysis side reaction in a similar way as in the case of other unstable drugs [26].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and chromatographic characterization of triflusal delivery systems prepared by using supercritical impregnation technologies

Argemí

López‐Periago

Domingo

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Spectroscopic and chromatographic characterization of triflusal delivery systems prepared by using supercritical impregnation technologies

Argemí

López‐Periago

Domingo

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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“…In addition, while the induction of members of the TBX2 subfamily by Aza was replicated across different NSCLC cell lines, we had noted variability in the extent of induction of the genes by Aza within the same cell line across different experiments. This variability is likely attributable to the biodegradable nature of Aza [14,27,28]. It is also noteworthy, that our analyses and findings do not preclude potential cross-talk, and/or antagonism between the four members of the TBX2 subfamily.…”

Section: Discussionmentioning

confidence: 77%

Epigenetic Suppression of the T-box Subfamily 2 (TBX2) in Human Non-Small Cell Lung Cancer

Nehme

Rahal

Sinjab

et al. 2019

IJMS

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(1) The TBX2 subfamily of transcription factors (TBXs 2, 3, 4 and 5) are markedly down-regulated in human non-small cell lung cancer (NSCLC) and exert tumor suppressor effects in lung malignancy. Yet, mechanisms underlying suppressed expression of the TBX2 subfamily in NSCLC are elusive. Here, we interrogated probable epigenetic mechanisms in suppressed expression of the TBX2 subfamily in human NSCLC. (2) TBX2 subfamily gene expression and methylation levels in NSCLC and normal lung tissues were surveyed using publicly available RNA-sequence and genome-wide methylation datasets. Methylation β-values of the four genes were statistically compared between NSCLCs and normal lung tissues, correlated with gene expression levels, and interrogated with clinicopathological variables. Expression and methylation levels of TBXs were quantified in NSCLC cells using real-time PCR and methylation-specific PCR assays, respectively. Effects of the DNA methyltransferase inhibitor 5-azacytidine (Aza) on TBX2 subfamily expression were assessed in NSCLC cells. Impact of TBX2 subfamily expression on Aza-treated cells was evaluated by RNA interference. (3) All four TBXs were significantly hypermethylated in NSCLCs relative to normal lung tissues (p < 0.05). Methylation β-values of the genes, with exception of TBX2, were significantly inversely correlated with corresponding mRNA expression levels (p < 0.05). We found no statistically significant differences in hypermethylation levels of the TBX2 subfamily by clinicopathological features including stage and tobacco history. Expression levels of the TBX genes were overall suppressed in NSCLC cells relative to normal alveolar cells. Members of the subfamily were significantly hypermethylated in all tested NSCLC cell lines relative to normal alveolar cells. Treatment with Aza induced the expression of the TBX2 subfamily concomitant with NSCLC cell growth inhibition. Further, simultaneous knockdown of the four TBX genes markedly reduced anti-growth effects of Aza in NSCLC cells. (4) Our study sheds light on new epigenetic profiles in the molecular pathogenesis of human NSCLC.

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“… Take into consideration the pH and temperature of the culture medium at the time the inhibitor is applied. It has been reported that 5-AzaC is moderately stable in acidic solutions while rapidly decomposing in alkaline media and that degradation is accelerated dramatically with increasing temperature [ 112 ]. …”

Section: The Use Of 5-azacytidine During Somatic Embryogenesismentioning

confidence: 99%

5-Azacytidine: A Promoter of Epigenetic Changes in the Quest to Improve Plant Somatic Embryogenesis

Osorio-Montalvo

Sáenz

De‐la‐Peña

2018

IJMS

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Somatic embryogenesis (SE) is a widely studied process due to its biotechnological potential to generate large quantities of plants in short time frames and from different sources of explants. The success of SE depends on many factors, such as the nature of the explant, the microenvironment generated by in vitro culture conditions, and the regulation of gene expression, among others. Epigenetics has recently been identified as an important factor influencing SE outcome. DNA methylation is one of the most studied epigenetic mechanisms due to its essential role in gene expression, and its participation in SE is crucial. DNA methylation levels can be modified through the use of drugs such as 5-Azacytidine (5-AzaC), an inhibitor of DNA methylation, which has been used during SE protocols. The balance between hypomethylation and hypermethylation seems to be the key to SE success. Here, we discuss the most prominent recent research on the role of 5-AzaC in the regulation of DNA methylation, highlighting its importance during the SE process. Also, the molecular implications that this inhibitor might have for the increase or decrease in the embryogenic potential of various explants are reviewed.

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Study of the degradation of 5-azacytidine as a model of unstable drugs using a stopped-flow method and further data analysis with multivariate curve resolution

Cited by 23 publications

References 15 publications

Spectroscopic and chromatographic characterization of triflusal delivery systems prepared by using supercritical impregnation technologies

Spectroscopic and chromatographic characterization of triflusal delivery systems prepared by using supercritical impregnation technologies

Epigenetic Suppression of the T-box Subfamily 2 (TBX2) in Human Non-Small Cell Lung Cancer

5-Azacytidine: A Promoter of Epigenetic Changes in the Quest to Improve Plant Somatic Embryogenesis

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