Study of the degradation mechanism of Chinese historic silk (Bombyx mori) for the purpose of conservation

Li, Meiying; Zhao, Yang; Tong, Tong; Hou, Xiaohui; Fang, Bei-Song; Wu, Shunqing; Shen, Xinyu; Tong, Hua

doi:10.1016/j.polymdegradstab.2012.12.021

Cited by 68 publications

(47 citation statements)

References 45 publications

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“…1,2 During the past several decades, the identification of archaeological silks has been achieved using a variety of analytical methods, including optical and electron microscopies, capillary electrophoresis-mass spectrometry, FTIR spectroscopy, microbeam synchrotron radiation diffraction and nuclear magnetic resonance. [3][4][5][6][7] However, external contamination, degradation of silk fibroin and different biological sources (i.e.…”

Section: Introductionmentioning

confidence: 99%

Identification of Ancient Silk Using an Enzyme-linked Immunosorbent Assay and Immuno-fluorescence Microscopy

et al. 2015

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The identification of ancient silk is of great importance in both archaeology and academia. In the present work, a specific antibody having the characteristics of low cost, easy operation and extensive applicability was developed directly through immunizing rabbits with complete antigen (silk fibroin, SF). Then, antibody-based immunoassays, i.e. enzyme-linked immunosorbent assay (ELISA) and immuno-fluorescence microscopy (IFM), were established and conducted in tandem to identify the corresponding protein in ancient silks. The anti-SF antibody exhibits high sensitivity and specificity for the identification of modern and ancient silks. The detection limit of the ELISA method is about 0.1 ng/mL, and no cross-reactions with other possible interference antigens have been noted. IFM makes it possible to localize target proteins in archaeological samples, and also ensure the reliability of the ELISA results. Based on these advantages, immunological techniques have the potential to become powerful analytical tools at archaeological sites and conservation science laboratories.

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Section: Introductionmentioning

confidence: 99%

Identification of Ancient Silk Using an Enzyme-linked Immunosorbent Assay and Immuno-fluorescence Microscopy

et al. 2015

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“…4 In the past, the degradation kinetics and mechanism of ancient silk and artificially aged silk were studied, which were aged by UV-irradiation, heating and modified humidity methods. [5][6][7][8] However, research concerning the enzymatic degradation of silk fabrics was limited. After being buried in the ground for centuries, unearthed silk fabrics were bestrewed by various microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…According to the literatures, 1,3,6,7 it was known that the photooxidizing, thermal, hydrolyzing degradation of silk fibroin were related to the changes with amino acid residues and breaking of the inter or intra chains bonds. 10 However, it was still unknown how the changes of enzymatic degradation occurred.…”

Section: Introductionmentioning

confidence: 99%

Application of Electron Paramagnetic Resonance and Solid-state 13C Nuclear Magnetic Resonance of Cross-polarization/Magic Angle Spinning to Study Enzymatic Degradation of Silk Fabrics

et al. 2017

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The enzymatic degradation of silk by protease XIV has been investigated by using scanning electron microscopy (SEM), Fourier transfer infrared spectroscopy (FTIR), solid-state 13 C nuclear magnetic resonance of cross-polarization/magic angle spinning ( 13 C CP/MAS solid state NMR) and electron paramagnetic resonance (EPR). Micro-morphology of protease XIV aged samples showed that microfilaments were stripped out from the surface of silk fibers. The results of FTIR and 13 C CP/MAS solid-state NMR indicated that the enzymatic degradation process could be divided into two stages. The EPR spectra indicated that the enzymatic degradation process was related to the free radical with the g-factor value of 2.0043. We also proposed that at the first degradation stage, the free radicals were apt to lose activities due to the loose structure of the non-crystalline region; at the second degradation stage, the free radicals produced in the crystalline region tended to be stored.

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“…To spot possible degradation pathways, a thermal ageing in 90°C in oxygen atmosphere was applied, coupled with different humidity levels and in presence of VOCs. These factors are known to affect art materials at a different rate [18,30,33,40,49,51,[53][54][55]. The deterioration of silk fibres causes brittleness of the textile, resulting in loss of mechanical endurance.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the impact of different exogenous factors on silk textiles deterioration with use of size exclusion chromatography

et al. 2016

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Size exclusion chromatography (SEC), especially coupled with multiple angle laser light scattering detector (MALLS) is a powerful tool in diagnostics of deterioration of historic and art objects to evaluate their condition. In this paper, SEC-UV-MALLS-DRI technique was applied to study degradation of silk fibroin samples (Bombyx mori) artificially aged under various conditions: in the presence of oxygen, in different amount of water vapour and in volatile organic products (VOCs), all at temperature of 90°C. Conditions were chosen in such a way that it mimicked real conditions of textiles' storing during exhibitions and in show cases. The influence of temperature, moisture and VOCs content on the state of silk textiles was examined with the use of size exclusion chromatography. Pseudo-zero-order Ekenstam equation was applied to study degradation rates of fibroin with use of the approximated values of DP of fibroin.

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Study of the degradation mechanism of Chinese historic silk (Bombyx mori) for the purpose of conservation

Cited by 68 publications

References 45 publications

Identification of Ancient Silk Using an Enzyme-linked Immunosorbent Assay and Immuno-fluorescence Microscopy

Identification of Ancient Silk Using an Enzyme-linked Immunosorbent Assay and Immuno-fluorescence Microscopy

Application of Electron Paramagnetic Resonance and Solid-state 13C Nuclear Magnetic Resonance of Cross-polarization/Magic Angle Spinning to Study Enzymatic Degradation of Silk Fabrics

Evaluating the impact of different exogenous factors on silk textiles deterioration with use of size exclusion chromatography

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