Study of the conformational behaviour of trehalose mycolates by FT-IR spectroscopy

Migliardo, F.; Bourdreux, Yann; Buchotte, Marie; Doisneau, Gilles; Beau, Jean‐Marie; Bayan, Nicolas

doi:10.1016/j.chemphyslip.2019.104789

Cited by 7 publications

(5 citation statements)

References 67 publications

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“…The structural–functional groups found in the BC and HC materials included C=C, O–H, C–O, C=O, C–H, Si–O–Al, Si–O–Si, Si–O, N–H, C–OH, CH 2 and C–N. It is evident that some functional groups, such as C–H and C=O (between 1413 and 1462 cm −1 ) were shared by BC and HC materials 86 , C–O and O–H (1033 cm -1 ) 87 . For BC-based materials, some peaks appeared with increasing kaolinite, such as; Si–O–Si groups at 465 and 469 cm −1 88 , Si–O at 791 cm −1 89 and C–O, C–N at 1083 cm −1 90 , 91 .…”

Section: Resultsmentioning

confidence: 91%

Impacts of kaolinite enrichment on biochar and hydrochar characterization, stability, toxicity, and maize germination and growth

Al-Swadi,

Al-Farraj,

Al-Wabel

et al. 2024

Sci Rep

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In this study, biochar (BC) and hydrochar (HC) composites were synthesized with natural kaolinite clay and their properties, stability, carbon (C) sequestration potential, polycyclic aromatic hydrocarbons (PAHs) toxicity, and impacts on maize germination and growth were explored. Conocarpus waste was pretreated with 0%, 10%, and 20% kaolinite and pyrolyzed to produce BCs (BC, BCK10, and BCK20, respectively), while hydrothermalized to produce HCs (HC, HCK10, and HCK20, respectively). The synthesized materials were characterized using X-ray diffraction, scanning electron microscope analyses, Fourier transform infrared, thermogravimetric analysis, surface area, proximate analyses, and chemical analysis to investigate the distinction in physiochemical and structural characteristics. The BCs showed higher C contents (85.73–92.50%) as compared to HCs (58.81–61.11%). The BCs demonstrated a higher thermal stability, aromaticity, and C sequestration potential than HCs. Kaolinite enriched-BCs showed the highest cation exchange capacity than pristine BC (34.97% higher in BCK10 and 38.04% higher in BCK20 than pristine BC), while surface area was the highest in kaolinite composited HCs (202.8% higher in HCK10 and 190.2% higher in HCK20 than pristine HC). The recalcitrance index (R50) speculated a higher recalcitrance for BC, BCK10, and BCK20 (R50 > 0.7), minimal degradability for HCK10 and HCK20 (0.5 < R50 < 0.7), and higher degradability for biomass and HC (R50 < 0.5). Overall, increasing the kaolinite enrichment percentage significantly enhanced the thermal stability and C sequestration potential of charred materials, which may be attributed to changes in the structural arrangements. The ∑ total PAHs concentration in the synthesized materials were below the USEPA’s suggested limits, indicating their safe use as soil amendments. Germination indices reflected positive impacts of synthesized charred materials on maize germination and growth. Therefore, we propose that kaolinite-composited BCs and HCs could be considered as efficient and cost-effective soil amendments for improving plant growth.

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Section: Resultsmentioning

confidence: 91%

Impacts of kaolinite enrichment on biochar and hydrochar characterization, stability, toxicity, and maize germination and growth

Al-Swadi,

Al-Farraj,

Al-Wabel

et al. 2024

Sci Rep

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“…To address this question, we first decided to test either purified or synthetic TMM molecules as substrates in the reaction. Purified TMM (pTMM) was prepared from our bacterial mycomembrane extract as described in the Materials and Methods section and synthetic TMM (sTMM) was obtained as previously described ( 20 ). Both preparations (see Supplementary Figure 1) of TMM are easily resuspended in aqueous solution and, as shown in Figure 1d, are readily used by MytC to mycoloylate PorA.…”

Section: Resultsmentioning

confidence: 99%

Synthetic mycolates derivatives as molecular tools to decipher protein mycoloylation, a unique post-translational modification in bacteria

Lesur,

Zhang,

Dautin

et al. 2024

Preprint

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Protein mycoloylation is a newly characterized post-translational modification (PTM) specifically found in Corynebacteriales, an order of bacteria that includes numerous human pathogens. Their envelope is composed of a unique outer membrane, the so-called mycomembrane made of very-long chain fatty acids, named mycolic acids. Recently, some mycomembrane proteins including PorA have been unambiguously shown to be covalently modified with mycolic acids in the model organism Corynebacterium glutamicum by a mechanism that relies on the mycoloyltransferase MytC. This PTM represents the first example of protein O-acylation in prokaryotes and the first example of protein modification by mycolic acid. Through the design and synthesis of trehalose monomycolate (TMM) analogs, we prove that i) MytC is the mycoloyltransferase directly involved in this PTM, ii) TMM, but not TDM, is a suitable mycolate donor for PorA mycoloylation, iii) MytC is able to discriminate between an acyl and a mycoloyl chain in vitro unlike other trehalose mycoloyltransferases. We also solved the structure of MytC acyl-enzyme obtained with a soluble short TMM analogs which constitutes the first mycoloyltransferase structure with a covalently linked to an authentic mycolic acid moiety. These data highlight the great conformational flexibility of the active site of MytC during the reaction cycle and pave the way for a better understanding of the catalytic mechanism of all members of the mycoloyltransferase family including the essential Antigen85 enzymes in Mycobacteria.

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“…In biomass (BM), a broad band in the range of 3300-3800 cm −1 indicates the existence of O-H bonding [41], which persisted throughout hydrothermal carbonization and disappeared during pyrolysis. Additionally, BCs and HCs shared some functional groups, including C-H and C=O (between 1413 and 1462 cm −1 [42]. Some peaks were detected in BCs and HCs, such as Si-O-Si groups at 465 and 469 cm −1 , representing the presence of kaolinite [43].…”

Section: Synthesized Materials Characteristicsmentioning

confidence: 97%

Kaolinite-Composited Biochar and Hydrochar as Low-Cost Adsorbents for the Removal of Cadmium, Copper, Lead, and Zinc from Aqueous Solutions

Al-Swadi,

Al-Farraj,

Al-Wabel

et al. 2023

Sustainability

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During the last decade, due to an increase in anthropogenic activities, a higher environmental accumulation of heavy metals has been found, which has resulted in disturbed biogeochemical balance. Many kinds of remediation techniques have been practiced to mitigate heavy metal toxicity in the aqueous phase; however, adsorption is the most commonly accepted technique for efficient heavy metal removal. In this study, conocarpus waste was pretreated with 0%, 10%, and 20% kaolinite and pyrolyzed at 600 °C for 1 h to synthesize biochars (BC, BCK10, and BCK20, respectively), while hydrothermalized at 200 °C for 6 h to synthesize hydrochars (HC, HCK10, and HCK20, respectively). After characterization, synthesized materials were employed for the removal of cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) from contaminated water. Experimental data was further subjected to isotherm and kinetic models to estimate the adsorption mechanism. Among all the tested adsorbents, kaolinite-synthesized materials revealed comparatively higher adsorption compared to pristine materials. It was found that pH 7 was optimum for the maximum removal of tested heavy metals. Adsorption of tested heavy metals was well explained by Langmuir and Freundlich isotherms, while pseudo-second order and Elovich kinetics models fitted well for adsorption kinetics. The maximum adsorption capacity, as predicted by the Langmuir isotherm, was the highest for BCK20 (63.19 mg g−1 for Cd, 228.05 mg g−1 for Cu, 248.33 mg g−1 for Pb, and 45.79 mg g−1 for Zn) compared to the other tested materials, and for HCK20 (31.93 mg g−1 for Cd, 181.78 mg g−1 for Cu, 231.85 mg g−1 for Pb, and 45.72 mg g−1), it was higher than pristine HC. Isotherm and kinetics modeling data indicated that multiple mechanisms were involved in Cd, Cu, Pb, and Zn removal, such as chemisorption and electrostatic interactions. The amount of oxygen-containing surface functional groups and SiO2 particles could be responsible for the maximum adsorption of heavy metals by BCK20 and HCK20. Our findings suggest that biochar, hydrochar, and their kaolinite-modified composites possess the excellent potential to remove heavy metals from contaminated aqueous media, and could be further applied to treat wastewater to mitigate heavy metal toxicity for a sustainable environment.

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Study of the conformational behaviour of trehalose mycolates by FT-IR spectroscopy

Cited by 7 publications

References 67 publications

Impacts of kaolinite enrichment on biochar and hydrochar characterization, stability, toxicity, and maize germination and growth

Impacts of kaolinite enrichment on biochar and hydrochar characterization, stability, toxicity, and maize germination and growth

Synthetic mycolates derivatives as molecular tools to decipher protein mycoloylation, a unique post-translational modification in bacteria

Kaolinite-Composited Biochar and Hydrochar as Low-Cost Adsorbents for the Removal of Cadmium, Copper, Lead, and Zinc from Aqueous Solutions

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