Study of the chemical structure of exopolysaccharide produced from streptomycete and its effect as an attenuate for antineoplastic drug 5-fluorouracil that induced gastrointestinal toxicity in rats

Mahmoud, Manal G.; Selim, Manal S.; Mohamed, Sahar S.; Hassan, Amal I.; Abdal-Aziz, Samia A.

doi:10.1080/10495398.2019.1610416

Cited by 10 publications

(6 citation statements)

References 61 publications

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“…Meanwhile, a significant reduction was detected in the 5-FU + RU group compared to the 5-FU group (Table 4). Mahmoud et al 2020). In the current research, the liver damage was induced by 5-FU in male Wistar rats and RU was found to be a hepatoprotective agent against this toxicity.…”

Section: Morphometric Resultsmentioning

confidence: 99%

Role of Platelet-activating factor and HO-1 in mediating the protective effect of rupatadine against 5-fluorouracil-induced hepatotoxicity in rats

Khalaf

Hafez

Abdalla

et al. 2022

Environ Sci Pollut Res

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5-fluorouracil (5-FU) is a widely used chemotherapeutic drug, but its hepatotoxicity challenges its clinical use. Thus, searching for a hepatoprotective agent is highly required to prevent the accompanied hepatic hazards. The current study aimed to investigate the potential benefit and mechanisms of action of rupatadine (RU), a Platelet-activating factor (PAF) antagonist, in the prevention of 5-FU-related hepatotoxicity in rats. Hepatotoxicity was developed in male albino rats by a single 5-FU (150 mg/kg) intra-peritoneal injection on the 7th day of the experiment. RU (3 mg/kg/day) was orally administrated to the rodents for 10 days. Hepatic toxicity was assessed by measuring both liver and body weights, serum alanine aminotransferase and aspartate aminotransferase (ALT and AST), hepatic oxidative stress parameters (malondialdehyde (MDA), nitric oxide levels (NOx), reduced glutathione (GSH), superoxide dismutase (SOD)), and heme oxygenase-1 (HO-1). Inflammatory markers expressions (inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNFα), interleukins; IL-1B, IL-6), the apoptotic marker (caspase-3), and PAF were measured in the hepatic tissue. 5-FU-induced hepatotoxicity was proved by the biochemical along with histopathological assessments. RU ameliorated 5-FU-induced liver damage as proved by the improved serum ALT, AST, and hepatic oxidative stress parameters, the attenuated expression of hepatic pro-inflammatory cytokines and PAF, and the up-regulation of HO-1. Therefore, it can be concluded that RU pretreatment exerted a hepatoprotective effect against 5-FU-induced liver damage through both its powerful anti-inflammatory, antioxidant, and anti-apoptotic effect.

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Section: Morphometric Resultsmentioning

confidence: 99%

Role of Platelet-activating factor and HO-1 in mediating the protective effect of rupatadine against 5-fluorouracil-induced hepatotoxicity in rats

Khalaf

Hafez

Abdalla

et al. 2022

Environ Sci Pollut Res

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“…While, no antifungal activity has been detected against Aspergillus niger. Bacterial biofilms are intricate populations of bacteria adhered to surfaces and held together by self-produced polymer matrixes, which mostly consist of secreted proteins, extracellular DNAs, and polysaccharides [5]. Bacterial biofilms have been found to contribute significantly to bacterial persistence and are a source of nosocomial infection that are engaged in a variety of infectious illnesses [45].…”

Section: Discussionmentioning

confidence: 99%

“…and streptomycetes. That had two forms an attachment shape to the cell wall called a capsular polysaccharide (CPS) or a free form known as exopolysaccharide that was released into culture (EPS) [4][5][6]. Sulfate polysaccharide is a form of polysaccharide with sulfate groups on the hydroxyl group [3,7].…”

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confidence: 99%

Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

Mohamed

Awady

Abdelhamid

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background Carbohydrates are known as the main natural products of life activities. Results Streptomyces rochie strain OF1 isolated from a mangrove tree produced exopolysaccharide S5 (EPSS5) (14.2 gl−1) containing uronic acid 21.98% sulfate content of 11.65 mg/ml, and a viscosity of 1.35 mm2/s. while total hexose amine content was 24.72%. The high performance liquid chromatography (HPLC) analysis of mono sugars revealed that EPS was composed of manouronic acid, glucuronic acid, xylose, and fructose at a molar ratio of 1.0:0.5:1.0:2.0, respectively. It showed that the whole antioxidant activity was 92.06%. It showed antibacterial activity against Staphylococcus aureus, and E. coli, MRSA and Klebsiella pneumoniae. But, EPSS5 displayed low antifungal activity against Candida albicans. While no antifungal activity has been detected against Aspergillus niger. EPSS5 has antibiofilm action that is noticeable toward S. aureus with an inhibition ratio of biofilm up to 50%. Effect of EPS on serum levels of TNF-α and COX2 by 2 fold and 1.9 fold of EPS reduced serum levels of Tumor necrosis factor-α (TNF-α) by 38%, 12%, 49%, and Cyclooxygenase-2 (COX2) by 61%, 34%, and 62%, respectively. By affected of EPSS5 on arthritis in rats stimulated by carrageenan. Conclusions Administration of EPS ameliorated carrageen-induced elevation in inflammatory mediators; TNF-α/COX and suppressed the expressions of metalloproteinase 9 (MMP9) by 68%, 86%, and 75% correspondingly in comparison to the group of carrageenans. Then again, therapy involving a high dose only reduced MMP9 level by 57%, compared to free drug suggesting that EPSS5 is a good inhibitor of the MMP9, as it brought MMP9 back to normal levels via the signaling pathway.

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“…Detailed studies should also be performed to evaluate their safety via the application of targeted high-throughput screening strategies [ 271 ]. Only limited research has been performed in vivo, supporting probiotic EPSs’ ability to alleviate intestinal inflammation via gut microbiota modulation [ 272 , 273 , 274 ], contribute to the maintenance of the epithelial barrier [ 275 ], exert anticancer effects [ 276 , 277 ], and attenuate 5-FU-induced toxicity in animal models [ 278 ]. Nevertheless, there is still a lack of clinical evidence to proceed into human administration.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Probiotic-Derived Bioactive Compounds in Colorectal Cancer Treatment

Thoda

Touraki

2023

Microorganisms

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Colorectal cancer (CRC) is a multifactorial disease with increased morbidity and mortality rates globally. Despite advanced chemotherapeutic approaches for the treatment of CRC, low survival rates due to the regular occurrence of drug resistance and deleterious side effects render the need for alternative anticancer agents imperative. Accumulating evidence supports that gut microbiota imbalance precedes the establishment of carcinogenesis, subsequently contributing to cancer progression and response to anticancer therapy. Manipulation of the gut microbiota composition via the administration of probiotic-derived bioactive compounds has gradually attained the interest of scientific communities as a novel therapeutic strategy for CRC. These compounds encompass miscellaneous metabolic secreted products of probiotics, including bacteriocins, short-chain fatty acids (SCFAs), lactate, exopolysaccharides (EPSs), biosurfactants, and bacterial peptides, with profound anti-inflammatory and antiproliferative properties. This review provides a classification of postbiotic types and a comprehensive summary of the current state of research on their biological role against CRC. It also describes how their intricate interaction with the gut microbiota regulates the proper function of the intestinal barrier, thus eliminating gut dysbiosis and CRC development. Finally, it discusses the future perspectives in precision-medicine approaches as well as the challenges of their synthesis and optimization of administration in clinical studies.

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Study of the chemical structure of exopolysaccharide produced from streptomycete and its effect as an attenuate for antineoplastic drug 5-fluorouracil that induced gastrointestinal toxicity in rats

Cited by 10 publications

References 61 publications

Role of Platelet-activating factor and HO-1 in mediating the protective effect of rupatadine against 5-fluorouracil-induced hepatotoxicity in rats

Role of Platelet-activating factor and HO-1 in mediating the protective effect of rupatadine against 5-fluorouracil-induced hepatotoxicity in rats

Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

Probiotic-Derived Bioactive Compounds in Colorectal Cancer Treatment

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