Study of the cellular proliferation kinetics of friend leukemia

Erythroleukemic Differentiation

“…Initially consid ered to be a reticulum cell sarcoma (17,18) associated with a reactive erythroblastosis (19), it was suggested in 1962 by Zajdela (20), on the basis of both pathological studies and radio-iron uptake, that the primary disease initiated by Friend virus complex was a neoplastic proliferation of ery thropoietic elements. With both the Friend and the Mirand variants, the daily output of young red blood cells in infected animals is significantly augmented (24), and is associated with considerable ineffective erythropoie sis, a term for premature death of young nucleated erythroid cells (25,26). With both the Friend and the Mirand variants, the daily output of young red blood cells in infected animals is significantly augmented (24), and is associated with considerable ineffective erythropoie sis, a term for premature death of young nucleated erythroid cells (25,26).…”

Studies of bone and soft‐tissue tumours induced in rats with radioactive cerium chloride

“…Seven animals were killed 1 h after injection of 3H-TdR, the others at different times, but we did not have enough animals at each point with the same type of tumour to make PLM curves for each type. Serial sections of the tumours were made and prepared for autoradiography in our laboratory as described before (Smadja-Joffe et al, 1973). Because of the heterogenicity of the labelling index in the preparations due to the coexistence of necrotic, non-proliferative zones and zones of ossification, tumours were divided into 2-mm zones and the labelling index (LI) was determined in each one.…”

Study of Cell Death in Friend Leukaemia

“…In an earlier publication, the following parameters have been calculated (Smadja-Joffe et al, 1973): T, = 7-6 hr gf = 0.64 I$ = 0-94 It follows that 1 k, =0.94 x lnl-64 x -7.6 kl= 0.0265 and *I24 --e-0.0265X24 = ,-0.6360 = 0.5294…”

“…They have shown that haemopoietic cells transformed by the virus multiply at the same speed as normal cells; in the case of Friend leukaemia the calculation based on the comparison of the real and potential doubling times of the leukaemic spleen has indicated that there is a cell loss factor of 94% during the exponential growth phase (Steel, 1968). Furthermore, a comparison of the level of production of leukaemic cells (proerythroblastic and erythroblastic) in the spleen, of their migration to other organs and of their rate of differentiation into erythrocytes has shown that at least two-thirds of the cells produced in the spleen die rapidly (Smadja-Joffe et al, 1973).…”