Study of the Biotransformation of Tongmai Formula by Human Intestinal Flora and Its Intestinal Permeability across the Caco-2 Cell Monolayer

Wu, Shuke; Xu, Wei; Wang, Furong; Yang, Xiu‐Wei

doi:10.3390/molecules201018704

Cited by 21 publications

(16 citation statements)

References 16 publications

(16 reference statements)

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“…It was reported that daidzin, isoflavone‐ O ‐glycoside, was easily hydrolyzed by glucosidases in small intestine (Miao et al, ). Meanwhile, puerarin and mirificin were reported to be transformed into the same aglycone, daidzein, by Caco‐2 cells (Wu, Wu, Wang, & Yang, ).…”

Section: Resultsmentioning

confidence: 99%

A UPLC–MS/MS approach for simultaneous determination of eight flavonoids in rat plasma, and its application to pharmacokinetic studies of Fu‐Zhu‐Jiang‐Tang tablet in rats

Tao

Chen

Cai

2016

Biomedical Chromatography

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The purpose of this study is to establish and validate a UPLC-MS/MS approach to determine eight flavonoids in biological samples and apply the method to pharmacokinetic study of Fu-Zhu-Jiang-Tang tablet. A Waters BEH C UPLC column was employed with methanol/0.1% formic acid-water as mobile phases. The mass analysis was carried out in a triple quadrupole mass spectrometer using multiple reaction monitoring with negative scan mode. A one-step protein precipitation by methanol was used to extract the analytes from blood. Eight major flavonoids were selected as markers. Our results showed that calibration curves for 3'-hydroxypuerarin, mirificin, puerarin, 3'-methoxypuerarin, daidzin, rutin, astragalin and daidzein displayed good linear regression (r > 0.9986). The intra-day and inter-day precisions (RSD) of the eight flavonoids at high, medium and low levels were <8.03% and the bias of the accuracies ranged from -5.20 to 6.75%.The extraction recoveries of the eight flavonoids were from 91.4 to 100.5% and the matrix effects ranged from 89.8 to 103.8%. The validated approach was successfully applied to a pharmacokinetic study in Sprague-Dawley rats after oral administration of FZJT tablet. Double peaks were emerged in curves of mean plasma concentration for 3'-methoxypuerarin, which was reported for the first time.

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Section: Resultsmentioning

confidence: 99%

A UPLC–MS/MS approach for simultaneous determination of eight flavonoids in rat plasma, and its application to pharmacokinetic studies of Fu‐Zhu‐Jiang‐Tang tablet in rats

Tao

Chen

Cai

2016

Biomedical Chromatography

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“…In our previous study [28], the pharmacokinetics of PUE administered orally in mice at three different concentrations (100, 200, and 300 mg/kg) showed nonlinear characteristics, probably because PUE absorption has an inhibitory effect on its concentration [29]. We showed here that the AUC0-360 min in blood and various tissues increased as the dose increased, indicating that PUE may exert a dose-dependent effect in the range of 20-80 mg/kg.…”

Section: Groupmentioning

confidence: 48%

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

Kong

Wang

Shi

et al. 2017

Preprint

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Puerarin (PUE) is a compound isolated from the roots of Pueraria lobata. We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and striatum. The plasma and tissue distribution kinetic characteristics following a single injection of PUE (20, 40, and 80 mg/kg) were calculated using a non-compartment model. In the high-dose (80 mg/kg) and medium-dose (40 mg/kg) groups, the kinetic profile of PUE in blood and kidney samples showed two absorption peaks, while that of the other tissues showed only one peak. In the low-dose (20 mg/kg) group, there was only one peak, irrespective of the sample type. Pharmacokinetic parameters such as the area under the curve, Cmax, and Tmax varied according to the administered dose. AUC and Cmax values increased dose-dependently. PUE was widely distributed in areas of the brain such as the hippocampus, cerebral cortex, and striatum, providing a foundation for guiding the use of PUE in the treatment of cerebral ischaemic stroke and neurodegenerative diseases.

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“…In our previous study [29], the pharmacokinetics of PUE administered orally in mice at three different concentrations (100, 200 and 300 mg/kg) showed nonlinear characteristics, probably because PUE absorption has an inhibitory effect on its concentration [30]. We showed here that the AUC 0–360 min in blood and various tissues increased as the dose increased, indicating that PUE may exert a dose-dependent effect in the range of 20–80 mg/kg.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

et al. 2017

View full text Add to dashboard Cite

Puerarin (PUE) is a compound isolated from the roots of Pueraria lobata. We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and striatum. The plasma and tissue distribution kinetic characteristics following a single injection of PUE (20, 40 and 80 mg/kg) were calculated using a non-compartment model. In the high-dose (80 mg/kg) and medium-dose (40 mg/kg) groups, the kinetic profile of PUE in blood and kidney samples showed two absorption peaks, while that of the other tissues showed only one peak. In the low-dose (20 mg/kg) group, there was only one peak, irrespective of the sample type. Pharmacokinetic parameters, such as the area under the curve, Cmax, and Tmax varied according to the administered dose. AUC and Cmax values increased dose-dependently. PUE was widely distributed in areas of the brain such as the hippocampus, cerebral cortex, and striatum, providing a foundation for guiding the use of PUE in the treatment of cerebral ischaemic stroke and neurodegenerative diseases.

show abstract

Study of the Biotransformation of Tongmai Formula by Human Intestinal Flora and Its Intestinal Permeability across the Caco-2 Cell Monolayer

Cited by 21 publications

References 16 publications

A UPLC–MS/MS approach for simultaneous determination of eight flavonoids in rat plasma, and its application to pharmacokinetic studies of Fu‐Zhu‐Jiang‐Tang tablet in rats

A UPLC–MS/MS approach for simultaneous determination of eight flavonoids in rat plasma, and its application to pharmacokinetic studies of Fu‐Zhu‐Jiang‐Tang tablet in rats

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

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