SummaryA reversed-phase liquid chromatographic method with sodium dodecylsulphate-n-propanolwater as mobile phase has been used to separate and determine sixwater-soluble vitamins in l",,velve minutes. The analytical characteristics linear range, sensitivity, detection limits, and precision were evaluated. The lowest detection limits were those of nicotinic acid (not usually present in pharmaceutical products), 0.7 mg L 1, nicotinamicle, 1.3 mg L 1, and pyricloxine, 1.4 mg L 1. When the method was applied to the determination of the vitamins in pharmaceutical samples the values found agreed with those on the labels.