Study of the association of toll like-4 receptor gene polymorphism (rs1927911) and susceptibility to urinary tract infection among Iraqi patients

Abstract

Introduction and Aim: Toll-like receptors (TLRs) are cell innate immune receptors that explore microbial molecules. A quantity of single-nucleotide polymorphisms (SNPs) within the TLR genes have been related with altered susceptibility to infectious, inflammatory, and allergic diseases suggesting: This study was conducted to investigate the relationship between genetic polymorphism in TLR4 gene (rs1927911) with susceptibility to UTIs in a sample of Iraqi patients. Materials and Methods: Blood samples were collected from 220 patients who had UTIs and 69 healthy subjects as control group, during the period from October 2021 to March 2022, at the Rheumatology Department of Clinic Consultant at Al-Yarmouk Hospital. The genetic study included an extraction of genomic DNA and PCR technique, to detect the role of TLR4 gene in the variation site (rs1927911) as a risk factor for the incidence of UTIs. Results: The results of genotype distribution and allele frequency of rs1927911 indicated, CC (18.6 %), CT (70 %) and TT (11.4 %) among the patients while 57.9 % for CC and 26.1%; 16% for CT, TT genotypes respectively of healthy control. Heterozygous (CT) may be considered as a risk factor among UTIs patients. The results revealed significant differences between UTIs patients and healthy controls in serum levels TLR4, IL-6 IL-2, TNF-alpha and IL-10 at P 0.005. In addition, the data found a highly significant difference of serum level of TLR4 protein with patients who have a carrier CT genotype. Conclusion: our findings indicated that the carriers of CT genotype of TLR4 (rs1927911) were significantly more prone to UTIs.

Association between Single Nucleotide Polymorphism in the Promoter of CD14 Gene and Development of Urinary Tract Infection

“…The development of infection is triggered by the presence of bacterial infection and the increased production of proinflammatory cytokines and chemokines (AlChalabi et al 2020). Environmental and genetic factors control the inter-individual variations in the inflammatory response to bacterial infections of the urinary system (Wujcicka et al 2018) (Allami et al 2023). Inflammatory reactions to bacterial colonization, immune cells implicated in inflammation, the production of pro-inflammatory mediators, and the activation of innate and adaptive immunity can all be affected by genetic variables (Xu et al 2019)(AlChalabi et al 2016 Gene polymorphism refers to variations in DNA sequence among people, groups, or populations.…”