Study of the alleviation effects of a combination of <i>Lactobacillus rhamnosus</i> and inulin on mice with colitis

Liu, Zhijing; Liu, Fei; Wang, Wan; Sun, Changbao; Gao, Da Ming; Ma, Jiage; Hussain, Muhammad; Xu, Cong; Jiang, Zhanmei

doi:10.1039/c9fo02992c

Cited by 81 publications

(55 citation statements)

References 70 publications

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“…Proteobacteria was reported to be the main phylum of pathogenic bacteria, which promoted the production of excessive pro-inflammatory cytokines and related to IBD pathogenesis (24,42). Akkermansia was a confirmed probiotic with anti-inflammatory effect by improving neutrophil infiltration and alleviating intestinal inflammation (44)(45)(46). Bifidobacteria could exert an anti-inflammatory effect through induction of intestinal IL-10 and protect from Th1driven inflammation (42).…”

Section: Discussionmentioning

confidence: 99%

Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

et al. 2021

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Probiotics plays an important role in regulating gut microbiota and maintaining intestinal homeostasis. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect. However, the anti-inflammatory effect and mechanism of probiotics derived EVs on inflammatory bowel disease (IBD) remains unclear. In this study, the effect of Lactobacillus plantarum Q7-derived extracellular vesicles (Q7-EVs) on gut microbiota and intestinal inflammation was investigated in C57BL/6J mice. The results showed that Q7-EVs alleviated DSS-induced colitis symptoms, including colon shortening, bleeding, and body weight loss. Consumption of Q7-EVs reduced the degree of histological damage. DSS-upregulated proinflammatory cytokine levels including IL-6, IL-1β, IL-2 and TNF-α were reduced significantly by Q7-EVs (p < 0.05). 16S rRNA sequencing results showed that Q7-EVs improved the dysregulation of gut microbiota and promoted the diversity of gut microbiota. It was observed that the pro-inflammatory bacteria (Proteobacteria) were reduced and the anti-inflammatory bacteria (Bifidobacteria and Muribaculaceae) were increased. These findings indicated that Q7-EVs might alleviate DSS-induced ulcerative colitis by regulating the gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

et al. 2021

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“…Liu et al found that Lactobacillus rhamnosus combined with inulin can reduce DSS-induced colitis, reduce pathological damage of colon tissue, reduce DAI score, regulate the expression of inflammatory cytokines, and have anti-inflammatory effects on mice with enteritis. 25 Therefore, this study established a colitis animal test model to preliminarily explore the ability of a synbiotic composed of L. fermentum HFY06 and arabinoxylan to alleviate colitis.…”

Section: Discussionmentioning

confidence: 99%

A Mixture of Lactobacillus fermentum HFY06 and Arabinoxylan Ameliorates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice

Li¹,

Huang²,

Zhu³

et al. 2021

JIR

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Objective Colitis is one of the main gastrointestinal diseases threatening human health. Materials and Methods In this study, a synbiotic composed of arabinoxylan (AX) and Lactobacillus fermentum HFY06 was tested to determine its ability to relieve dextran sulfate sodium (DSS)-induced colitis. Results The experimental results showed that the synergistic effect of AX and L. fermentum HFY06 alleviated the weight loss of DSS-mediated colitis mice and lowered the disease activity index (DAI) score. Determination of biochemical indicators found that the synbiotic composed of AX and L. fermentum HFY06 increased the body’s antioxidant capacity and reduced inflammation. The histopathological examination results showed that the colonic crypts of the mice in the model group were disordered, goblet cells were lost, and the mucous membrane was severely damaged. However, the combination of AX and L. fermentum HFY06 can significantly reverse the histopathological changes in the colon mediated by DSS. The gene expression of colon tissue was further determined, and the results showed that the synergistic effect of AX and L. fermentum HFY06 inhibited the activation of the NF-κB signaling pathway, downregulated the mRNA expression levels of nuclear factor-κB-p65 (NF-κBp65), upregulated the mRNA expression of NF-κB inhibitor-α (IκB-α), inhibited the release of cytokines tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2), and exerted anti-colitis effects. Conclusion This study shows that the synbiotic composed of AX and L. fermentum HFY06 has the potential to prevent and treat colitis.

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“…Last year, researchers found that the pasteurized A. muciniphila or one of its specific outer membrane proteins can alleviate colitis and prevent the occurrence of colitis-associated colorectal cancer by modulating the levels of CD8 + cytotoxic T lymphocytes [ 43 ]. A. muciniphila can also exert an anti-inflammatory effect by preventing neutrophil infiltration and reducing intestinal inflammation [ 44 ]. In this study, HBBG intake significantly increased the relative abundance of A. muciniphila in the intestine.…”

Section: Discussionmentioning

confidence: 99%

β-Glucan Extracted from Highland Barley Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice

Chen

Tian

et al. 2021

Molecules

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Inflammatory bowel disease (IBD), which significantly affects human health, has two primary presentations: Crohn’s disease and ulcerative colitis (UC). Highland barley is the most common food crop for Tibetans and contains much more β-glucan than any other crop. Highland barley β-glucan (HBBG) can relieve the gastrointestinal dysfunction and promote intestines health. This study aimed to evaluate whether HBBG can relieve UC in mice. A mouse model of UC was established by adding 2% dextran sulfate sodium (DSS) to drinking water for 1 week. UC was alleviated after the introduction of the HBBG diet, as indicated by reductions in the disease activity index (DAI) score, histopathological damage, and the concentration of colonic myeloperoxidase (MPO), along with an improvement in colonic atrophy. Furthermore, we found that HBBG can increase the relative transcriptional levels of genes encoding ZO-1, claudin-1, occludin, and mucin2 (MUC2), thereby reducing intestinal permeability. Additionally, HBBG maintained the balance of proinflammatory and anti-inflammatory cytokines and modulated the structure of the intestinal flora.

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Study of the alleviation effects of a combination of Lactobacillus rhamnosus and inulin on mice with colitis

Abstract: Ulcerative colitis (UC) is a common inflammatory bowel disease (IBD) that has serious harmful effects on human health.

Cited by 81 publications

References 70 publications

Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

A Mixture of Lactobacillus fermentum HFY06 and Arabinoxylan Ameliorates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice

β-Glucan Extracted from Highland Barley Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice

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