Study of the allergenic fractions of latex

Chambeyron, C.; Dry, J.; Leynadier, F.; Pecquet, C.; Thao, Tran Xuan

doi:10.1111/j.1398-9995.1992.tb05094.x

Cited by 51 publications

(16 citation statements)

References 17 publications

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“…Significant variation in the number of proteins and in the amount of proteins in various extracts of natural rubber latex has been reported [3]. A few of these peptides have been purified and characterized, e.g.…”

Section: Discussionmentioning

confidence: 99%

A Murine Model of Latex Allergy-Induced Airway Hyperreactivity

Thakker

Xia

Rickaby

et al. 1999

Lung

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Sensitization to latex proteins can cause immediate IgE mast cell-mediated reactions. Health care workers have been found to be particularly at risk because of high exposure. Latex allergy can be produced in mice as demonstrated by IgE and eosinophil responses. Thus the mouse is a potential animal model for studying this disease, but the airway response to latex sensitization in mice has not been evaluated previously. In the present study, we immunized BALB/c mice intranasally with nonammoniated latex proteins. Animals were anesthetized, and lung mechanics were evaluated plethysmographically. Changes in pulmonary conductance (GL) and compliance (Cdyn) were measured in response to a nonspecific challenge with methacholine or to a direct challenge with intravenous latex antigen. Latex sensitization resulted in elevated levels of IgE and latex-specific IgG1 as well as interstitial infiltrates consistent with an allergic response. The methacholine dose-response ED50 for GL was 116.4 microg for the control mice and fell significantly to 20.9 microg for latex-sensitized mice. The ED50 calculated for Cdyn was also significantly lower after latex sensitization. The GL in latex-sensitized mice challenged with latex antigen fell significantly from a prechallenge value of 1.87 +/- 0.41 (S.E.) to 0. 198 +/- 0.03 ml x s-1 x cmH2O after latex antigen challenge. The results indicate that latex-sensitized mice did exhibit increased airway reactivity in the methacholine challenge test. The latex allergic response in mice is unique in that direct challenge with latex antigen itself also resulted in a significant airway response.

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Section: Discussionmentioning

confidence: 99%

A Murine Model of Latex Allergy-Induced Airway Hyperreactivity

Thakker

Xia

Rickaby

et al. 1999

Lung

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“…Immunoelectrophoretic studies conducted by several groups of investigators have identified IgE-binding proteins with molecular weights ranging 2-100 kDa in raw NRL and eluates from NRL finished products [9,[27][28][29][30][31][32][33][34] results. Furthermore, discrepancies may have resulted from differences in the immune sera (e.g.…”

Section: Nrl Allergensmentioning

confidence: 99%

Occupational asthma caused by natural rubber latex

Vandenplas¹

1995

Eur Respir J

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O Oc cc cu up pa at ti io on na al l a as st th hm ma a c ca au us se ed d b by y n na at tu ur ra al l r ru ub bb be er r l la at te ex x Recent studies indicate that asthma is a frequent manifestation of NRL allergy among workers manufacturing NRL materials and among health-care providers using NRL gloves. NRL-induced asthma should receive increasing attention as it can lead to permanent respiratory sequelae and occupational disability. The need for early and accurate diagnosis is outlined and the different diagnostic approaches are reviewed. Specific issues pertaining to the management of affected subjects and to the prevention of exposure to airborne NRL are discussed.Areas of future research should include: 1) further characterization of relevant NRL allergens; 2) development and validation of methods for quantitative assessment of allergen content in NRL devices and workplace environments; 3) standardization of allergen extracts used for diagnostic purposes; 4) prospective evaluation of the natural history and risk factors of NRL-induced asthma; and 5) analysis of effectiveness and cost of preventive strategies.

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“…A 14.6-kD monomer of this protein was identified as the REF by its amino acid sequence and it was reported to in duce symptoms in latex-allergic patients and to block com pletely latex-specific IgE antibodies in all 13 sera from adult latex-allergic patients tested. Given the large number o f al lergens found by other investigators in natural rubber latex (NRL) [5][6][7][8] it is, however, difficult to conceive that only one glove allergen, such as REF, could be responsible for hypersensitivity symptoms in most if not all latex-allergic patients.…”

Section: Introductionmentioning

confidence: 99%

Significance of Rubber Elongation Factor as a Latex Allergen

Alenius

Kalkkinen

Yip³

et al. 1996

Int Arch Allergy Immunol

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IgE-mediated hypersensitivity to proteins eluting from surgical gloves and other natural rubber products is a well-recognized allergic condition with special impact to health care workers and sensitized patients. Since rubber elongation factor (REF) has been suggested to be the major latex allergen (Hev b 1) we purified REF from rubber particles using electrophoresis and electroelution and reevaluated the occurrence of IgE antibodies to purified REF. The purified protein was subjected to tryptic digestion, peptide separation and amino acid sequencing. Amino acid sequences of 9 tryptic peptides from the purified 14-kD protein gave 100% homology to REF. In immunoblotting, sera from 4 of 6 latex-allergic children with spina bifida or other congenital anomalies exhibited IgE antibodies against REF whereas only 1 serum from the 30 other latex-allergic patients had similar antibodies. In IgE ELISA 8/45 (18%) latex-allergic patients had IgE antibodies to purified REF, whereas 30 (67%) of the same patients had IgE antibodies to purified prohevein, another rubber protein, recently shown to be a major natural rubber latex allergen. The observed overall low frequency of IgE antibodies to purified REF, as measured by two specific IgE assays, indicates that REF is one but not the most significant allergen among the natural rubber latex proteins.

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Study of the allergenic fractions of latex

Cited by 51 publications

References 17 publications

A Murine Model of Latex Allergy-Induced Airway Hyperreactivity

A Murine Model of Latex Allergy-Induced Airway Hyperreactivity

Occupational asthma caused by natural rubber latex

Significance of Rubber Elongation Factor as a Latex Allergen

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