Study of the 3D Structure of a Hydrogel with Gold Nanoparticles by Cryogenic Electron Microscopy

Mikhutkin, A. A.; Kamyshinsky, Roman; Kuznetsov, Nikita M.; Лебедев-Степанов, П. В.; Stepko, A. S.; Vasiliev, Alexander L.

doi:10.1134/s2635167622030119

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“…Undesirable structural changes and/or particle aggregation can be prevented by using cryogenic transmission electron microscopy (cryo-TEM), according to which samples are preliminarily frozen rather than dried. Cryo-TEM is successfully employed to study not only biological objects, but also metallic NPs [12,13], detonation nanodiamonds [14], etc. However, the detection limit, the complexity of selecting freezing conditions, and subsequent processing of images, as well as the high cost of equipment, limit the widespread use of cryo-TEM.…”

Section: Introductionmentioning

confidence: 99%

The Role of Integrated Approach in the Determination of Nanoparticle Sizes in Dispersions

Kuznetсova

Kuznetsov²,

Kalinin³

et al. 2022

Colloid J

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Aqueous dispersions of gold and silver nanoparticles (NPs) stabilized with sodium citrate, as well as polymeric NPs based on poly(lactide-co-glycolide) (70 : 30 mol %) stabilized with poly(vinyl alcohol) have been obtained. The sizes of NPs have been determined by transmission electron microscopy (TEM), dynamic light scattering (DLS), and analytical ultracentrifugation (AUC). It has been shown that the diameter of both metallic and polymeric particles measured by TEM is significantly lower than their hydrodynamic diameter Dh determined by DLS. Moreover, it has been found that the Dh value obtained for the studied particles from DLS distributions is overestimated compared to that determined by AUC, because the AUC method is less sensitive to the particle size polydispersity and the presence of large objects in a dispersion.

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Section: Introductionmentioning

confidence: 99%