Study of taxonomic status of some specimens of subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus) from Western Mongolia based on mtDNA markers

Vodolazhsky, D. I.; Yakovlev, R.V.; Stradomsky, B.V.

doi:10.23885/1814-3326-2011-7-1-81-82

Cited by 3 publications

(4 citation statements)

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“…Eastern Turkish-Caucasian clade ( Polyommatus ripartii paralcestis ) is strongly differentiated and appears as a group close to Polyommatus demavendi . Complicated taxonomy and phylogeography of Polyommatus ripartii have recently been topics of several specific studies and publications (Vila et al 2010, Vodolazhsky et al 2011, Dincă et al 2013a, Przybyłowicz et al 2014) and are out of the focus of the present paper. The sequences obtained in our study confirm that Balkan samples represent one of the major clades within Polyommatus ripartii populations, thus Polyommatus ripartii pelopi is confirmed as a valid subspecies.…”

Section: Taxonomymentioning

confidence: 99%

Karyosystematics and molecular taxonomy of the anomalous blue butterflies (Lepidoptera, Lycaenidae) from the Balkan Peninsula

Vishnevskaya¹,

Saifitdinova²,

Lukhtanov³

2016

CCG

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The Balkan Peninsula represents one of the hottest biodiversity spots in Europe. However, the invertebrate fauna of this region is still insufficiently investigated, even in respect of such well-studied organisms as Lepidoptera. Here we use a combination of chromosomal, molecular and morphological markers to rearrange the group of so-called anomalous blue butterflies (also known as ‘brown complex’ of the subgenus Agrodiaetus Hübner, [1822] and as the Polyommatus (Agrodiaetus) admetus (Esper, 1783) species group) and to reveal its cryptic taxonomic structure. We demonstrate that Polyommatus aroaniensis (Brown, 1976) is not as widespread in the Balkans as was previously thought. In fact, it has a dot-like distribution range restricted to the Peloponnese Peninsula in South Greece. Polyommatus orphicus Kolev, 2005 is not as closely related to the Turkish species Polyommatus dantchenkoi (Lukhtanov & Wiemers, 2003) as was supposed earlier. Instead, it is a Balkan endemic represented by two subspecies: Polyommatus orphicus orphicus (Bulgaria) and Polyommatus orphicus eleniae Coutsis & De Prins, 2005 (Northern Greece). Polyommatus ripartii (Freyer, 1830) is represented in the Balkans by an endemic subspecies Polyommatus ripartii pelopi. The traditionally recognized Polyommatus admetus (Esper, 1783) is shown to be a heterogeneous complex and is divided into Polyommatus admetus sensu stricto (the Balkans and west Turkey) and Polyommatus yeranyani (Dantchenko & Lukhtanov, 2005) (east Turkey, Armenia, Azerbaijan and Iran). Polyommatus nephohiptamenos (Brown & Coutsis, 1978) is confirmed to be a species with a dot-like distribution range in Northern Greece. Finally, from Central Greece (Timfristos and Parnassos mountains) we describe Polyommatus timfristos Lukhtanov, Vishnevskaya & Shapoval, sp. n. which differs by its haploid chromosome number (n=38) from the closely related and morphologically similar Polyommatus aroaniensis (n=47-48) and Polyommatus orphicus (n=41-42). We provide chromosomal evidence for three separate south Balkan Pleistocene refugia (Peloponnesse, Central Greece and Northern Greece/South Bulgaria) and stress the biogeographic importance of Central Greece as a place of diversification. Then we argue that the data obtained have direct implications for butterfly karyology, taxonomy, biogeography and conservation.

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Section: Taxonomymentioning

confidence: 99%

Karyosystematics and molecular taxonomy of the anomalous blue butterflies (Lepidoptera, Lycaenidae) from the Balkan Peninsula

Vishnevskaya¹,

Saifitdinova²,

Lukhtanov³

2016

CCG

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“…COI sequences were obtained from 44 specimens representing the P. damone species group and from two samples [P. damon (Denis et Schiffermüller, 1775) and P. icarus (Rottemburg, 1975)] which were selected as outgroup (Table 1). Legs were sampled from these specimens, and sequence data from the DNA barcode region of COI were obtained at the Canadian Centre for DNA Barcoding (CCDB, Biodiversity Institute of Ontario, University of Guelph) using protocols described in Hajibabaei et al (2005), Ivanova et al (2006) and deWaard et al (2008) We also used 31 published COI sequences (Wiemers 2003;Kandul et al 2004Kandul et al , 2007Lukhtanov et al 2005Lukhtanov et al , 2009Vodolazhsky et al 2011; Vodolazhsky and Stradomsky 2012) which were downloaded from GenBank (Table 1).…”

Section: Molecular Methods and Dna Barcode Analysismentioning

confidence: 99%

“…The taxa of the complex were previously revised by Staudinger (1899), Forster (1956Forster ( , 1960, Dantchenko and Lukhtanov (1993) and Dantchenko (1997). There are also limited molecular (Wiemers 2003;Kandul et al 2004Kandul et al , 2007Lukhtanov et al 2005Lukhtanov et al , 2009Vodolazhsky et al 2011;Vodolazhsky and Stradomsky 2012) and chromosomal (Lukhtanov 1989;Kandul 1997;Lukhtanov et al 1997;Lukhtanov and Dantchenko 2002a;Lukhtanov et al 2005) data for a few taxa of the complex. However, the complex has never been systematically studied by using chromosomal and molecular markers, although such an approach is considered as an essential requirement for revealing taxonomic structure in the subgenus Agrodiaetus (Lukhtanov and Dantchenko 2002b;Kandul et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal and DNA barcode analysis of the Polyommatus (Agrodiaetus) damone (Eversmann, 1841) species complex (Lepidoptera, Lycaenidae)

Lukhtanov¹,

Dantchenko²

2021

CCG

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The Polyommatus (Agrodiaetus) damone (Eversmann, 1841) species complex comprises from 5 to 8 species distributed in southeastern Europe and southern Siberia. Here we used chromosomal and DNA-barcode markers in order to test the taxonomic hypotheses previously suggested for this complex. We revealed that all taxa within this group demonstrate chromosomal stasis and share the same or very similar haploid chromosome number (n = 66 or n = 67). This finding is unexpected since the karyotypes are known to be very diverse and species-specific within the other taxa of the subgenus Agrodiaetus Hübner, 1822. Analysis of the mitochondrial gene COI revealed six diverged clusters of individuals within the complex. Each cluster has a specific geographic distribution and is characterized by distinct morphological features in the wing pattern. The clusters mostly (but not always) correlate with traditionally recognized species. As a result of our study, we describe a new subspecies P. (A.) iphigenides zarmitanussubsp. nov. from Uzbekistan and Tajikistan and show that the taxon originally described as Lycaena kindermanni var. melania Staudinger, 1886 represents a subspecies P. (A.) iphigenides melanius (Staudinger, 1886). Polyommatus (A.) samusi Korb, 2017 (syn. nov.) and P. (A.) melanius komarovi Korb, 2017 (syn. nov.) are considered here as junior subjective synonyms of P. (A.) iphigenides iphigenides (Staudinger, 1886).

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“…This subgenus has been studied relatively well with respect to molecular markers, and for many species, multi-locus molecular data are available, including such genes as mitochondrial COI , tRNA-leu , COII , cytochrome b and NADH dehydrogenase sequences and nuclear 5.8S rDNA , ITS2 , 28S rDNA and EF1-α sequences [ 4 , 17 , 18 , 27 , 29 , 30 , 31 ]. At the same time, for many taxa, especially for rare species from Turkey, Iran, Pakistan and Afghanistan, only mitochondrial DNA barcodes are available [ 32 ], or the molecular data are absent.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Structure Revealed through Combining DNA Barcodes with Multi-Gene Data for Agrodiaetus Blue Butterflies (Lepidoptera, Lycaenidae)

Lukhtanov,

Shapoval,

Dantchenko

et al. 2023

Insects

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The need for multi-gene analysis in evolutionary and taxonomic studies is generally accepted. However, the sequencing of multiple genes is not always possible. For various reasons, short mitochondrial DNA barcodes are the only source of molecular information for some species in many genera, although multi-locus data are available for other species of the same genera. In particular, such situation exists in the species-rich butterfly subgenus Polyommatus (Agrodiaetus). Here, we analyzed the partitioning of this subgenus into species groups by using three data sets. The first data set was represented by short mitochondrial DNA barcodes for all analyzed samples. The second and third data sets were represented by a combination of short mitochondrial DNA barcodes for part of the taxa with longer mitochondrial sequences COI + tRNA-Leu + COII (data set 2) and with longer mitochondrial COI + tRNA-Leu + COII and nuclear 5.8S rDNA + ITS2 + 28S rDNA sequences (data set 3) for the remaining species. We showed that the DNA barcoding approach (data set 1) failed to reveal the phylogenetic structure, resulting in numerous polytomies in the tree obtained. Combined analysis of the mitochondrial and nuclear sequences (data sets 2 and 3) revealed the species groups and the position within these species groups, even for the taxa for which only short DNA barcodes were available.

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Study of taxonomic status of some specimens of subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus) from Western Mongolia based on mtDNA markers

Cited by 3 publications

References 0 publications

Karyosystematics and molecular taxonomy of the anomalous blue butterflies (Lepidoptera, Lycaenidae) from the Balkan Peninsula

Karyosystematics and molecular taxonomy of the anomalous blue butterflies (Lepidoptera, Lycaenidae) from the Balkan Peninsula

Chromosomal and DNA barcode analysis of the Polyommatus (Agrodiaetus) damone (Eversmann, 1841) species complex (Lepidoptera, Lycaenidae)

Phylogenetic Structure Revealed through Combining DNA Barcodes with Multi-Gene Data for Agrodiaetus Blue Butterflies (Lepidoptera, Lycaenidae)

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