Study of Surface Structure Changes for Selected Ceramics Used in the CAD/CAM System on the Degree of Microbial Colonization, In Vitro Tests

Abstract

In the article has been presented an analysis of susceptibility of selected dental materials, made in the CAD/CAM technology. The morphology and structural properties of selected dental materials and their composites were determined by using XRPD (X-ray powder diffraction) techniques, as well as the IR (infrared) spectroscopy. Moreover, an adhesion as well as development of biofilm by oral microorganisms has been studied. It has been shown that a degree of the biofilm development on the tested dental materials depended on microorganism genus and species. Streptococcus mutans has demonstrated the best adhesion to the tested materials in comparison with Candida albicans and Lactobacillus rhamnosus. However, the sintered materials such as IPS e.max® and the polished IPS e.max® have showed the best “anti-adhesive properties” in relation to S. mutans and L. rhamnosus that have not formed the biofilm on the polished IPS e.max® sample. Furthermore, S. mutans have not formed the biofilm on both surfaces. On the contrary to S. mutans and L. rhamnosus, C. albicans has demonstrated the adhesive properties in relation to the above-mentioned surfaces. Moreover, in contrast to S. mutans and C. albicans, L. rhamnosus has not formed the biofilm on the polished IPS Empress material.

Review on Polymer, Ceramic and Composite Materials for CAD/CAM Indirect Restorations in Dentistry—Application, Mechanical Characteristics and Comparison

“…The adhesion and development of microorganisms being part of biofilm on different materials used for CAD/CAM was studied. As a result, it can be claimed that tested sintered materials, such as IPS e.max and polished IPS e.max, showed the best “anti-adhesive properties” with respect to Streptococcus mutans and Lactobacillus rhamnosus [ 99 ]. In another study, materials such as VITA CAD-, Celtra Duo, IPS e.max CAD and VITA YZ were tested to determine the cytotoxic effects and collagen type I secretions on human gingival fibroblasts.…”

Key Properties of a Bioactive Ag-SiO2/TiO2 Coating on NiTi Shape Memory Alloy as Necessary at the Development of a New Class of Biomedical Materials

“…To respond to these issues, a series of literature studies showed that surface roughness or wettability should be considered in the context of the biological response [ 64 ]. Here, the significant increase in the surface roughness of more than 0.2 mm and/or of the surface-free energy facilitated the formation of unwanted bacterial biofilm [ 65 , 66 ]. However, the material with a surface roughness that ranged between 10–100 nm did not affect the total amount of human cell adhesion [ 67 , 68 ].…”

Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study

“…From the culture of strains, a suspension of 0.5 McFarland density in a liquid Sabourada Bulion Brain Heart Infusion-BHI and MRS-de Man, Rogosa and Sharpe medium (BioMaxima S.A., Lublin, Poland) was prepared for C. albicans, S. mutans and L. rhamnosus, respectively [2], [4]. The so-prepared suspensions were incubated for 24 h with haemostatic agents with initial concentration of aluminium chloride equal to 1000 µg/ml at 37 °C, in aerobic, anaerobic and microaerophilic conditions.…”