Study of sponge gourd ascorbate peroxidase and winter squash superoxide dismutase under respective flooding and chilling stresses

Lin, Kuan–Hung; Kuo, Wen-Shuo; Chiang, Chih-Ming; Hsiung, Tung-Chuan; Chiang, Ming‐Chang; Lo, Hsiao-Feng

doi:10.1016/j.scienta.2013.08.016

Cited by 23 publications

(15 citation statements)

References 24 publications

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“…This results in the destruction of the membranes and as a consequence an increase in lipid peroxidation (Lin et al 2013). However, in the present study MDA levels increased only slightly in the leaf, while oscillation occurred in the root, which suggests that waterlogging did not caused significant damage to membrane lipids as reflected by MDA content.…”

Section: Resultscontrasting

confidence: 74%

Antioxidant enzymes efficiently control leaf and root cell damage in young Euterpe oleracea plants exposed to waterlogging

Pereira

Silva

Filho

et al. 2015

Ind J Plant Physiol.

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Euterpe oleracea (Mart.), an Amazonian palm, is of economic importance due to the production of energy drinks. Plants exposed to waterlogging are prone to damage at the cellular level and cause irreversible metabolic dysfunctions leading to cell death. This study was aimed to study cell damages and to investigate enzyme activities related to antioxidant system in this specie. The experimental design was in factorial randomized with two water conditions (waterlogging and control) and five evaluation times (0, 6, 12, 18, and 24 days). The parameters measured were electrolyte leakage (EL), contents of hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA) and glutathione (GSH), and enzymatic activities of catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX) in leaf and root tissues. Waterlogging did not promote significant modifications in EL, MDA and H 2 O 2 in both the tissues evaluated, and a minor alteration in GSH was observed only in the roots. Significant increases in the activity of CAT, APX and POX were observed in leaf and roots, at early stages. It can thus be concluded that CAT, APX and POX were efficient in controlling EL and MDA in leaf and root of E. oleracea plants under waterlogging.

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Section: Resultscontrasting

confidence: 74%

Antioxidant enzymes efficiently control leaf and root cell damage in young Euterpe oleracea plants exposed to waterlogging

Pereira

Silva

Filho

et al. 2015

Ind J Plant Physiol.

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“…The POD activities increased by 21.8%, 35.5%, 37.8% and 42.8% in WT, T-1, T-2 and T-3, respectively; whereas the SOD activities increased by 224.7%, 262.3%, 241.7% and 258.9% in WT, T1, T2 and T3, respectively. These results indicated that the SOD activities were much more induced in plants than the POD activities, which is in line with previous studies that have shown that SOD activity in plants is more sensitive to chilling stress than POD activity [40]. Our quantitative analysis of the POD and SOD activities confirmed this and revealed that transgenic lines had more accumulation of ROS irrespective of the higher activities of SOD and POD.…”

Section: Physiological Changes Involved In Chilling-low Light Stresssupporting

confidence: 91%

“…SOD and POD are considered to be the most important protective enzymes for removal of ROS. The degree of cell injury upon exposure to ROS initiated under chilling-low irradiance has been studied in various plants based on the peroxidase activity as an indicator [40]. In this study, under normal growth conditions, there was no significant difference in the activities of SOD and POD between the transgenic lines and WT plants.…”

Section: Physiological Changes Involved In Chilling-low Light Stressmentioning

confidence: 75%

Molecular cloning and characterization of ClZE, a zeaxanthin epoxidase gene in watermelon (Citrullus lanatus)

Liu

Yao

et al. 2017

Biotechnology & Biotechnological Equipment

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In this study, a watermelon (Citrullus lanatus) zeaxanthin epoxidase gene, ClZE, was isolated by reverse transcription-polymerase chain reaction (PCR) together with RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE). The full cDNA sequence of ClZE is 2535 bp in length containing a 1998 bp open reading frame (ORF) that encodes 665 amino acids. ClZE was shown to share high homology with the putative ZE genes in other plant species. Prediction analysis revealed that ClZE bears two large conservative domains, including Pyr_redox and FHA (forkheadassociated domain). Phylogenetic analysis suggested that ClZE shares high similarity (94.8%) with CsZE from Cucumis sativus, but is far from the ZE genes of other species. Prokaryotic expression indicated that ClZE possessed the apparent molecular mass consistent with its calculated molecular mass of 73.1 KDa. Based on quantitative real-time PCR (qRT-PCR), ClZE was shown to be down-regulated under chilling-low irradiance stress in watermelon leaves. Transgenic Arabidopsis lines harbouring ClZE were generated to test the gene function in mediating plant response to chilling stress. The results indicated that the transgenic lines exhibited decreased nonphotochemical quenching (NPQ) and maximum quantum efficiency of photosystem II (PSII) photochemistry (Fv/Fm), increased activities of peroxidase (POD), superoxide dismutase (SOD) and elevated content of malondialdehyde (MDA) relative to the wild type (WT) under chilling-low irradiance stress. In addition, overexpression of ClZE resulted in impaired xanthophyll cycle and aggravated PSII photoinhibition under chilling-low irradiance. All the results together suggested that ClZE plays major roles in regulating the photoinhibition behaviour.

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“…The SPAD value represents the relative CC of each leaf. The third fully expanded leaves were inserted into the measuring head and five readings taken from each leaf and averaged (Lin et al 2013). Data were collected from one leaf per plant and five plants per treatment.…”

Section: Determination Of Different Physiological and Biochemical Promentioning

confidence: 99%

Physiological and proteomic analysis in two wild tomato lines under waterlogging and high temperature stress

Lin

Syu

et al. 2015

J. Plant Biochem. Biotechnol.

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Tomato yields are reduced under waterlogging and high temperature stress condition. Ascorbic acid (ASA) was shown to be involved in tolerance to waterlogging and heat stresses in tomato. Among 44 wild tomato lines treated with waterlogging at 38°C, L6138 (Solanum peruvianum) showed highest ASA, shoot growth, chlorophyll content and chlorophyll fluorescence in comparison to other tomato lines. Further leaf proteins in L6138 and L0994 under waterlogging at 38°C for 72 h were analyzed by two-dimensional protein fractionation system. Different protein peaks were analyzed by comparative proteomic analysis and database searching. Fifty protein peaks expressed in response to stress treatment were identified, among which 27 proteins from L0994 and 17 proteins from L6138 were successfully sequenced. Differentially proteins expressed have major functions in protein structure maintenance, metabolism, secretion, translation, biosynthesis, signal transduction, degradation, and photosynthesis under stress. ASA might be played a role in tolerance to waterlogging and heat stress by maintaining RNA transcription, protein structure, and metabolism. In this study, we utilized a physiological and proteomic approach to discover the changes in protein expression profiles of tomatoes in response to heat and flood stresses.

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Study of sponge gourd ascorbate peroxidase and winter squash superoxide dismutase under respective flooding and chilling stresses

Cited by 23 publications

References 24 publications

Antioxidant enzymes efficiently control leaf and root cell damage in young Euterpe oleracea plants exposed to waterlogging

Antioxidant enzymes efficiently control leaf and root cell damage in young Euterpe oleracea plants exposed to waterlogging

Molecular cloning and characterization of ClZE, a zeaxanthin epoxidase gene in watermelon (Citrullus lanatus)

Physiological and proteomic analysis in two wild tomato lines under waterlogging and high temperature stress

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