Study of solvent–protein coupling effects by neutron scattering

Varga, Balázs; Migliardo, F.; Takács, Enikő; Vértessy, Beáta G.; Magazù, Salvatore; Telling, Mark

doi:10.1007/s10867-009-9177-5

Cited by 16 publications

(16 citation statements)

References 33 publications

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“…In water the nonbonding electrons are the H-bond acceptors and the hydrogen atoms are the H-bond donors. As shown in Figure 2, the polarity of water molecules results in the attraction of the negative (oxygen) portion and positive (hydrogen) partial charges and is at the basis for the water hydrogen bonding which have many important implications on the properties of water and its relevant functions in biological systems [10][11][12][13].…”

Section: Soft Interaction In Amphiphiles Self-assemblymentioning

confidence: 99%

Amphiphiles Self-Assembly: Basic Concepts and Future Perspectives of Supramolecular Approaches

Lombardo

Киселев

Magazù

et al. 2015

Advances in Condensed Matter Physics

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Amphiphiles are synthetic or natural molecules with the ability to self-assemble into a wide variety of structures including micelles, vesicles, nanotubes, nanofibers, and lamellae. Self-assembly processes of amphiphiles have been widely used to mimic biological systems, such as assembly of lipids and proteins, while their integrated actions allow the performance of highly specific cellular functions which has paved a way for bottom-up bionanotechnology. While amphiphiles self-assembly has attracted considerable attention for decades due to their extensive applications in material science, drug and gene delivery, recent developments in nanoscience stimulated the combination of the simple approaches of amphiphile assembly with the advanced concept of supramolecular self-assembly for the development of more complex, hierarchical nanostructures. Introduction of stimulus responsive supramolecular amphiphile assembly-disassembly processes provides particularly novel approaches for impacting bionanotechnology applications. Leading examples of these novel self-assembly processes can be found, in fact, in biosystems where assemblies of different amphiphilic macrocomponents and their integrated actions allow the performance of highly specific biological functions. In this perspective, we summarize in this tutorial review the basic concept and recent research on self-assembly of traditional amphiphilic molecules (such as surfactants, amphiphile-like polymers, or lipids) and more recent concepts of supramolecular amphiphiles assembly which have become increasingly important in emerging nanotechnology.

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Section: Soft Interaction In Amphiphiles Self-assemblymentioning

confidence: 99%

Amphiphiles Self-Assembly: Basic Concepts and Future Perspectives of Supramolecular Approaches

Lombardo

Киселев

Magazù

et al. 2015

Advances in Condensed Matter Physics

Self Cite

401

256

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“…The peculiar polarisation of water molecules near the hydrophilic solute interfaces sensitively alters the liquid structure with respect to the structure usually found in bulk water. [18,19]. …”

Section: Soft Interactions Involved In Liposome Nanocarriersmentioning

confidence: 99%

Soft Interaction in Liposome Nanocarriers for Therapeutic Drug Delivery

et al. 2016

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The development of smart nanocarriers for the delivery of therapeutic drugs has experienced considerable expansion in recent decades, with the development of new medicines devoted to cancer treatment. In this respect a wide range of strategies can be developed by employing liposome nanocarriers with desired physico-chemical properties that, by exploiting a combination of a number of suitable soft interactions, can facilitate the transit through the biological barriers from the point of administration up to the site of drug action. As a result, the materials engineer has generated through the bottom up approach a variety of supramolecular nanocarriers for the encapsulation and controlled delivery of therapeutics which have revealed beneficial developments for stabilizing drug compounds, overcoming impediments to cellular and tissue uptake, and improving biodistribution of therapeutic compounds to target sites. Herein we present recent advances in liposome drug delivery by analyzing the main structural features of liposome nanocarriers which strongly influence their interaction in solution. More specifically, we will focus on the analysis of the relevant soft interactions involved in drug delivery processes which are responsible of main behaviour of soft nanocarriers in complex physiological fluids. Investigation of the interaction between liposomes at the molecular level can be considered an important platform for the modeling of the molecular recognition processes occurring between cells. Some relevant strategies to overcome the biological barriers during the drug delivery of the nanocarriers are presented which outline the main structure-properties relationships as well as their advantages (and drawbacks) in therapeutic and biomedical applications.

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“…Are they translational or rotational in nature? Such questions can uniquely be answered by quasielastic neutron scattering (QENS), thanks to the dependence of the observed relaxation times on the wavevector Q, which can not be accessed with other spectroscopy techniques such as NMR [15,16].…”

Section: Introductionmentioning

confidence: 99%

Dynamics of lysozyme and its hydration water under an electric field

et al. 2014

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The effects of a static electric field on the dynamics of lysozyme and its hydration water are investigated by means of incoherent quasi-elastic neutron scattering (QENS). Measurements were performed on lysozyme samples, hydrated respectively with heavy water (D 2 O) to capture the protein dynamics and with light water (H 2 O), to probe the dynamics of the hydration shell, in the temperature range from 210 < T < 260 K. The hydration fraction in both cases was about ∼ 0.38 gram of water per gram of dry protein.The field strengths investigated were respectively 0 kV/mm and 2 kV/mm (∼2 × 10 6 V/m) for the protein hydrated with D 2 O and 0 kV and 1 kV/mm for the H 2 O-hydrated counterpart. While the overall internal protons dynamics of the protein appears to be unaffected by the application of an electric field up to 2 kV/mm, likely due to the stronger intra-molecular interactions, there is also no appreciable quantitative enhancement of the diffusive dynamics of the hydration water, as would be anticipated based on our recent observations in water confined in silica pores under field values of 2.5 kV/mm. This may be due to the difference in surface interactions between water and the two adsorption hosts (silica and protein), or to the existence of a critical threshold field value E c ∼2-3 kV/mm for increased molecular diffusion, for which electrical breakdown is a limitation for our sample.

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Study of solvent–protein coupling effects by neutron scattering

Cited by 16 publications

References 33 publications

Amphiphiles Self-Assembly: Basic Concepts and Future Perspectives of Supramolecular Approaches

Amphiphiles Self-Assembly: Basic Concepts and Future Perspectives of Supramolecular Approaches

Soft Interaction in Liposome Nanocarriers for Therapeutic Drug Delivery

Dynamics of lysozyme and its hydration water under an electric field

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