Study of Several Alginate-Based Hydrogels for In Vitro 3D Cell Cultures

Jiao, Weijie; Xiao-hong, LI; Shan, Jingxin; Wang, Xiaohong

doi:10.3390/gels8030147

Cited by 10 publications

(11 citation statements)

References 58 publications

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“…It was shown that this thermosensitive hydrogel can be used as a three-dimensional platform with high biocompatibility and easy functionalization, and has great potential in the biomedical field. Similarly, Jiao et al [ 111 ] prepared sodium alginate-based composite hydrogels with good cell compatibility, which can be used for three-dimensional cell culture in vitro. Moreover, Nguyen et al [ 112 ] developed a co-culture system of ECM degradation products and MSCs with macrophages.…”

Section: Numerical Resultsmentioning

confidence: 99%

Modeling Tunable Fracture in Hydrogel Shell Structures for Biomedical Applications

Zhang

Qiu

Elkhodary

et al. 2022

Gels

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Hydrogels are nowadays widely used in various biomedical applications, and show great potential for the making of devices such as biosensors, drug- delivery vectors, carriers, or matrices for cell cultures in tissue engineering, etc. In these applications, due to the irregular complex surface of the human body or its organs/structures, the devices are often designed with a small thickness, and are required to be flexible when attached to biological surfaces. The devices will deform as driven by human motion and under external loading. In terms of mechanical modeling, most of these devices can be abstracted as shells. In this paper, we propose a mixed graph-finite element method (FEM) phase field approach to model the fracture of curved shells composed of hydrogels, for biomedical applications. We present herein examples for the fracture of a wearable biosensor, a membrane-coated drug, and a matrix for a cell culture, each made of a hydrogel. Used in combination with experimental material testing, our method opens a new pathway to the efficient modeling of fracture in biomedical devices with surfaces of arbitrary curvature, helping in the design of devices with tunable fracture properties.

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Section: Numerical Resultsmentioning

confidence: 99%

Modeling Tunable Fracture in Hydrogel Shell Structures for Biomedical Applications

Zhang

Qiu

Elkhodary

et al. 2022

Gels

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“…Thermo-responsive hydrogels are supramolecular hydrogels that undergo a gelation process via hydrophobic interaction. Hydrogels can undergo sol–gel phase transition because they consist of amphiphilic polymers with hydrophilic and hydrophobic components [ 73 ]. Hydrogels can undergo a gelation process from room temperature to body temperature (range of 25–37 °C), which is beneficial for their application in tissue engineering [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrogel-Based Biomaterial as a Scaffold for Gingival Regeneration: A Systematic Review of In Vitro Studies

et al. 2023

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Background: Hydrogel is considered a promising scaffold biomaterial for gingival regeneration. In vitro experiments were carried out to test new potential biomaterials for future clinical practice. The systematic review of such in vitro studies could synthesize evidence of the characteristics of the developing biomaterials. This systematic review aimed to identify and synthesize in vitro studies that assessed the hydrogel scaffold for gingival regeneration. Methods: Data on experimental studies on the physical and biological properties of hydrogel were synthesized. A systematic review of the PubMed, Embase, ScienceDirect, and Scopus databases was conducted according to the Preferred Reporting System for Systematic Reviews and Meta-Analyses (PRISMA) 2020 statement guidelines. In total, 12 original articles on the physical and biological properties of hydrogels for gingival regeneration, published in the last 10 years, were identified. Results: One study only performed physical property analyses, two studies only performed biological property analyses, and nine studies performed both physical and biological property analyses. The incorporation of various natural polymers such as collagen, chitosan, and hyaluronic acids improved the biomaterial characteristics. The use of synthetic polymers faced some drawbacks in their physical and biological properties. Peptides, such as growth factors and arginine–glycine–aspartic acid (RGD), can be used to enhance cell adhesion and migration. Based on the available primary studies, all studies successfully present the potential of hydrogel characteristics in vitro and highlight the essential biomaterial properties for future periodontal regenerative treatment.

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“…Alginate offers a fantastic toolset for design and optimisation, even though one system is likely to fit only some research or cell types [120]. The hydrogels used for in vitro 3D cell culturing have specific physicochemical characteristics, such as hardness, water holding capacity (WHC), swelling-erosion ratio, and swelling rate, to mirror the natural extracellular matrices (ECMs) found in living things [122].…”

Section: Cell Immobilisationmentioning

confidence: 99%

Alginate-Based Applications in Biotechnology with a Special Mention to Biosensors

Paul¹,

Markus²,

Kristollari³

et al. 2024

Biochemistry

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The exploitation of alginate and its composites as immobilisation support matrices in multiple applications remains a promising field that has the potential to create advanced functional materials from sustainable natural sources. They are non-toxic, allow sol-gel transformation, are biocompatible, have remarkable ion exchange properties, are biodegradable, and are amenable to chemical functionalisation. Alginate and its derived composites have numerous biotechnological and biomedical applications, including biomolecule or cell immobilisation, tissue engineering, drug delivery, wound dressing, and biosensors. Alginate can rapidly crosslink into a stable 3D water-insoluble network called hydrogel with polyvalent cations. Blending alginate with other materials to produce composite materials with improved or novel physicochemical properties remains an ongoing research endeavour. For instance, natural and synthetic polymers or nanoparticles have been incorporated into alginate-yielding composite material with enhanced physical strength, controlled porosity, improved interaction between the alginate support and the biomolecules, and the impartation of other features such as electrical and magnetic responsiveness, among others. Immobilisation strategies are discussed herein, including their innovations and future research perspectives.

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Study of Several Alginate-Based Hydrogels for In Vitro 3D Cell Cultures

Cited by 10 publications

References 58 publications

Modeling Tunable Fracture in Hydrogel Shell Structures for Biomedical Applications

Modeling Tunable Fracture in Hydrogel Shell Structures for Biomedical Applications

Hydrogel-Based Biomaterial as a Scaffold for Gingival Regeneration: A Systematic Review of In Vitro Studies

Alginate-Based Applications in Biotechnology with a Special Mention to Biosensors

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