Study of serum VEGF levels in patients with severe dengue infection admitted in a tertiary care hospital in Kolkata

Patra, Goutam; Saha, Bibhuti; Mukhopadhyay, Sumi

doi:10.1002/jmv.25529

Cited by 9 publications

(12 citation statements)

References 13 publications

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“…Nevertheless, the strong positive correlation between VEGF and IL-1RA serum levels was observed exclusively in non-neurological disease, suggesting a distinct mechanism of inflammation control in the neurological disorder. Elevated levels of serum VEGF were observed in children with viral encephalitis (51), in untreated patients with HIV-associated encephalopathy (52), and in patients with severe dengue (53,54). On the hand, EGF levels were increased in CSF of patients with neurological manifestations and did not show significant correlations with other molecules.…”

Section: Discussionmentioning

confidence: 94%

Cytokines and Soluble HLA-G Levels in the Acute and Recovery Phases of Arbovirus-Infected Brazilian Patients Exhibiting Neurological Complications

et al. 2021

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Severe neurological complications following arbovirus infections have been a major concern in seasonal outbreaks, as reported in the Northeast region of Brazil, where the same mosquito transmitted Zika (ZIKV), Dengue (DENV), and Chikungunya (CHIKV) viruses. In this study, we evaluated the levels of 36 soluble markers, including cytokines, chemokines, growth factors, and soluble HLA-G (Luminex and ELISA) in: i) serum and cerebrospinal fluid (CSF), during the acute phase and two years after the infection (recovery phase, only serum), ii) the relationship among all soluble molecules in serum and CSF, and iii) serum of infected patients without neurological complications, during the acute infection. Ten markers (sHLA-G, IL-10, IL-22, IL-8, MIP-1α, MIP-1β, MCP-1, HGF, VEGF, and IL-1RA) exhibited differential levels between the acute and recovery phases, with pronounced increases in MIP-1α (P<0.0001), MCP-1 (P<0.0001), HGF (P= 0.0001), and VEGF (P<0.0001) in the acute phase. Fourteen molecules (IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-13, IL-15, IL-17A, IFN-α, TNF, and G-CSF) exhibited distinct levels between arbovirus patients presenting or not neurological complications. IL-8, EGF, IL-6, and MCP-1 levels were increased in CSF, while RANTES and Eotaxin levels were higher in serum. Soluble serum (IL-22, RANTES, Eotaxin) and CSF (IL-8, EGF, IL-3) mediators may discriminate putative risks for neurological complications following arbovirus infections. Neurological complications were associated with the presence of a predominant inflammatory profile, whereas in non-complicated patients an anti-inflammatory profile may predominate. Mediators associated with neuroregeneration (EGF and IL-3) may be induced in response to neurological damage. Broad spectrum immune checkpoint molecules (sHLA-G) interact with cytokines, chemokines, and growth factors. The identification of soluble markers may be useful to monitor neurological complications and may aid in the development of novel therapies against neuroinflammation.

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Section: Discussionmentioning

confidence: 94%

Cytokines and Soluble HLA-G Levels in the Acute and Recovery Phases of Arbovirus-Infected Brazilian Patients Exhibiting Neurological Complications

et al. 2021

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“…VEGF was the most widely studied growth factor. A total of 33 studies analyzed VEGF, and 22 found increased levels of VEGF in patients with dengue, most of them severe dengue, compared with nonsevere dengue and/or healthy controls [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ], including 5 studies in children only [ 16 , 17 , 21 , 26 , 27 ]. This trend of increased levels was maintained regardless of the disease classification, age group, or sample type.…”

Section: Resultsmentioning

confidence: 99%

The Role of Growth Factors in the Pathogenesis of Dengue: A Scoping Review

2022

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Growth factors (GFs) have a role in tissue repair and in the modulation of the expression of inflammatory cells in damage caused by pathogens. This study aims to systematize the evidence on the role of GFs in the pathogenesis of dengue. This scoping review considered all published peer-reviewed studies in the MEDLINE and Embase databases. Ultimately, 58 studies that analyzed GFs in dengue patients, published between 1998 and 2021, were included. DENV-2 infection and secondary infection were more frequent in the patients studied. ELISA and multiplex immunoassay (Luminex) were the most used measurement techniques. Increased levels of vascular endothelial growth factor, granulocyte–macrophage colony-stimulating factor, granulocyte colony-stimulating factor, transforming growth factor beta, and hepatocyte growth factor as well as reduced levels of platelet-derived growth factor and epidermal growth factor were observed in severe dengue in most studies. Vascular endothelial growth factor and hepatocyte growth factor were identified as biomarkers of severity. In addition, there is evidence that the dengue virus can use the growth factor pathway to facilitate its entry into the cell and promote its viral replication. The use of tyrosine kinase inhibitors is an alternative treatment for dengue that is being studied.

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“…Statistical analysis was performed using the Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA free demo version 5.04) and all statistical methods were adopted from Patra et al, 2019 8,9 . Briefly, all data were subjected to ANOVA with post-hoc test for finding the significance of each study groups.…”

Section: Determination Of Serum Cytokine Titres In Serummentioning

confidence: 99%

“…a high level of IL6 and vasodilation agent vascular endothelial growth factor (VEGF) were noted in severe dengue patients 8,9 . In this study, we also investigate the status of another important vasodilating factor TNF-α.…”

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confidence: 99%

Increased levels of pentraxins protein and cytokines bear good association in patients with severe dengue infection

Patra¹,

Saha²,

Mukhopadhyay³

2021

Sci Rep

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Dengue is an arboviral infection with high rates of morbidity and mortality throughout the tropics and sub-tropics. This work studied the status of pentraxin (CRP/SAP) protein, ferritin, TNF-α and IL-1β levels in Dengue patients of different pathophysiological manifestations. Accordingly, clinically confirmed Dengue cases (n = 97) were enrolled and subsequently blood parameters were studied by Haematology cell counter and Biochemistry Autoanalyser. CRP, SAP, ferritin, TNF-α and IL-1β ELISA were done in all the samples by using standard ELISA kits. Statistical Analysis was done in all the experiments. The levels of CRP (p < 0.0001), SAP (p < 0.0001), ferritin (p < 0.0001), TNF-α (p < 0.0001) and IL-1β (p < 0.0001) were high in patients with Severe Dengue as compared to Dengue without warning signs. High levels of SGOT, SGPT and decreased platelet counts were found in severe patients as compared to Healthy donor. CRP/SAP as well as TNF-α/IL-1β were independently associated with both dengue severity and overall disease manifestation. Statistically significant increased CRP, SAP, ferritin, TNF-α and IL-1β titres were correlated in patients with severe clinical manifestations as compared to mild disease forms of dengue. Elevated levels of pentraxin, TNF-α/IL-1β in blood during dengue infection could act as an early predictor in Severe Dengue infection.

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Study of serum VEGF levels in patients with severe dengue infection admitted in a tertiary care hospital in Kolkata

Cited by 9 publications

References 13 publications

Cytokines and Soluble HLA-G Levels in the Acute and Recovery Phases of Arbovirus-Infected Brazilian Patients Exhibiting Neurological Complications

Cytokines and Soluble HLA-G Levels in the Acute and Recovery Phases of Arbovirus-Infected Brazilian Patients Exhibiting Neurological Complications

The Role of Growth Factors in the Pathogenesis of Dengue: A Scoping Review

Increased levels of pentraxins protein and cytokines bear good association in patients with severe dengue infection

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